167,601 results

1. pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA

   Plasmid

   #156447

   Purpose

   For transient expression of mouse SMC3 tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse SMC3 (Smc3 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCHAC-mt-mKeima

   Plasmid

   #72342

   Purpose

   retrovirus construct for stable expressing mt-mKeima

   Depositor

   Richard Youle

   Article

   Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

   Insert

   mt-mKeima
   

   Use

   Retroviral

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. p38-KTR-mStrawberry reporter

   Plasmid

   #158687

   Purpose

   This plasmid is a p38 pathway reporter. It has CMV promoter driving the expression of p38-KTR fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCVpuro-Bcl2

   Plasmid

   #191964

   Purpose

   Exogenous expression of Bcl2 WT

   Depositor

   Luca Scorrano

   Insert

   Bcl2 (BCL2 Human)
   

   Use

   Retroviral

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. OmpA-mNG-sfTq2ox

   Plasmid

   #124220

   Purpose

   Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

   Depositor

   Tanneke den Blaauwen

   Article

   Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

   Insert

   OmpA-mNG-sfTq2ox
   

   Expression

   Bacterial

   Available Since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lentiviral-TOP-dGFP-reporter

   Plasmid

   #14715

   Purpose

   Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   LEF-1/TCF-responsive promoter (Ctnnb1 )
   

   Use

   Lentiviral

   Tags

   d2 eGFP

   Expression

   Mammalian

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCMV-intron myc Rab2WT

   Plasmid

   #46779

   Purpose

   expression of WT Rab2a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB2 (RAB2A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-CFP

   Plasmid

   #11179

   Purpose

   Mammalian expression vector for expression of CFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Insert

   ECFP from Aequorea victoria
   

   Expression

   Mammalian

   Available Since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC18T-mini-Tn7T-Tp-ecfp

   Plasmid

   #65034

   Purpose

   TpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   ecfp
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mammalian expression: Z-lock cofilin dark state mutant

    Plasmid

    #141136

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Expression

    Mammalian

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcFLAG-FYN-WT

    Plasmid

    #74509

    Purpose

    Overexpression of FYN in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Chougule et al Oncotarget. 2016 Feb 2. doi: 10.18632/on

    Insert

    FYN (FYN Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cbh_v5 AAV-ABE N-terminal

    Plasmid

    #137177

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-ABE N-terminal
    

    Use

    AAV

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBtet-GH

    Plasmid

    #60498

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBR BsmAIM Kan + p15A BsaIR Amp

    Plasmid

    #206208

    Purpose

    Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    BsamAIM and BsaIR
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6/V5-DEST-RNF2

    Plasmid

    #31216

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    RNF2 (RNF2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-CAG-SomaGCaMP6f2 (AAV9)

    Viral Prep

    #158757-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Promoter

    CAG promoter

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

18. pSEED

    Plasmid

    #219658

    Purpose

    Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Empty SEED Cassette (3xP3-dsRed)
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-tmpknot-epegRNA-puro backbone

    Plasmid

    #214089

    Purpose

    Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF1a

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

    Plasmid

    #223209

    Purpose

    Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL10 (IL10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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22. MXS_BidirectionalCAG

    Plasmid

    #78175

    Purpose

    Bidirectional CAG promoter (compatible with the MXS Chaining Kit)

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    Bidirectional CAG Promoter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pmBanana-Hyg-CTCF-CtermNES-N1

    Plasmid

    #131851

    Purpose

    Expresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cells

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    CTCF (CTCF Human)
    

    Tags

    mBanana

    Expression

    Mammalian

    Mutation

    the C-terminus of CTCF is linked to HIV-Rev prote…

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHR-Traditional CAR (anti-CD19)

    Plasmid

    #192115

    Purpose

    Lentiviral delivery of Traditional CAR(anti-CD19)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
    

    Use

    Lentiviral

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3.1(+)Flag-His-ATM wt

    Plasmid

    #31985

    Purpose

    Mammalian expression of human ATM with Flag and His tags

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM wild-type (ATM Human)
    

    Tags

    Flag and HIS

    Expression

    Mammalian

    Mutation

    3' UTR of 400 nucl.

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

    Plasmid

    #156436

    Purpose

    For transient expression of human PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human PDS5B (PDS5B Human)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMKV057

    Plasmid

    #133312

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc+. WUS2, IPT
    

    Expression

    Plant

    Promoter

    AtUbi10, Nos, 35S

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP-Merlin

    Plasmid

    #84293

    Purpose

    Expression of wt Merlin

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223_STAT3_WT

    Plasmid

    #82235

    Purpose

    Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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31. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPD304 VPR-ZF43

    Plasmid

    #138840

    Purpose

    Constitutive expression of VPR-ZF43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CAG-Electra1-P2A-EGFP

    Plasmid

    #184935

    Purpose

    Expresses blue fluorescent protein Electra1 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-P2A-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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34. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)

    Viral Prep

    #112677-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

35. pOpen-DNA Topoisomerase 1B Vaccinia Virus

    Plasmid

    #165510

    Purpose

    Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    DNA Topoisomerase 1B Vaccinia Virus
    

    Use

    Synthetic Biology

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pT3-EF1a-c-Met

    Plasmid

    #31784

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBT375

    Plasmid

    #125616

    Purpose

    Expresses evoAPOBEC1-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max-NG
    

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti_PEmax-2A-MLH1dn-2A-BSD

    Plasmid

    #234072

    Purpose

    For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PEmax-2A-MLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF3164

    Plasmid

    #144640

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RUVBL1 (RUVBL1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRedF

    Plasmid

    #118057

    Purpose

    GBPart - RedF Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Red Firefly Luciferase CDS
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Mutation

    Red mutant S286Y

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge