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179,538 results

Showing: 7951 - 8000 of 179538 results

  3. pLNCX chick src K295R

    Plasmid
    #13659
    Depositor
    Joan Brugge
    Insert
    src K295R (SRC Chicken)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    K295R. Kinase dead mutant.
    Available since
    Jan. 4, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles

  5. pGEX-4T3-RhoA-Q63L

    Plasmid
    #12961
    Depositor
    Gary Bokoch
    Insert
    RhoA constitutively active (RHOA Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Q63L Constitutively active
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  12. pCX420

    Plasmid
    #229211
    Purpose
    Cytoplasmic-targeting Csm complex plasmid
    Depositor
    Jennifer Doudna
    Publication
    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6
    Expression
    Mammalian
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229211-plasmid-map-sequence-id-457182

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  13. pCMV-PE6e

    Plasmid
    #207855
    Purpose
    Mammalian expression of PE6e prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6e
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, K918A, K775R)
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    207855-plasmid-map-sequence-id-408162

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  17. pAAV-S5E2-dTom-nlsdTom (AAV9)

    Viral prep
    #135630-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Publication
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Tags
    dTomato
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only

  18. pEF1a-hMLH1NTD-NLS

    Plasmid
    #174826
    Purpose
    Mammalian expression of human MLH1NTD-NLS (codon optimized)
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human MLH1_NTD-NLS (codon optimized)
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    truncation of residues 1-335
    Promoter
    EF1a
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174826-plasmid-map-sequence-id-343633

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  21. pAAV-nEF-Coff/Fon-NpHR3.3-EYFP (AAV8)

    Viral prep
    #137154-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-NpHR3.3-EYFP (#137154). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-NpHR3.3-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of NpHR3.3-EYFP (inhibited in presence of Cre) for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Flp-dependent)
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only

  22. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV8)

    Viral prep
    #105553-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only

  23. Plenti-ARE-HSVTK-luc

    Plasmid
    #161789
    Purpose
    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers
    Depositor
    Hans-Guido Wendel
    Publication
    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi
    Inserts
    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    EF1a and Murine Gclc ARE
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    161789-plasmid-map-sequence-id-501011

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  26. AIMTOR T757

    Plasmid
    #140828
    Purpose
    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
    Depositor
    Anne Bonnieu
    Publication
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AIMTOR T757 (ULK1 Human)
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140828-plasmid-map-sequence-id-305141

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  27. pAAV-Ef1a-fDIO mCherry (AAV2)

    Viral prep
    #114471-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only

  29. pVE13300

    Plasmid
    #137715
    Purpose
    Expresses impLbCas12a in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    impLbCas12a
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137715-plasmid-map-sequence-id-274023

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  36. EasyCloneYALI- CRISPR/Cas9 Set

    Plasmid kit
    #1000000141
    Purpose
    Plasmids for CRISPR-based genome editing in Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Yeast Expression
    Editing type
    CRISPR
    Cloning type
    USER
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  39. pLX_TRC311

    Plasmid
    #113668
    Purpose
    Gateway destination vector for lentiviral expression
    Depositor
    John Doench
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    113668-plasmid-map-sequence-id-220922

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  41. pENN.AAV.CB7.CI.eGFP.WPRE.rBG (AAV9)

    Viral prep
    #105542-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.CB7.CI.eGFP.WPRE.rBG (#105542). In addition to the viral particles, you will also receive purified pENN.AAV.CB7.CI.eGFP.WPRE.rBG plasmid DNA. CB7-driven eGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CB7
    Tags
    EGFP
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only

  42. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (AAV9)

    Viral prep
    #105543-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (#105543). In addition to the viral particles, you will also receive purified pENN.AAV.cTNT.PI.eGFP.WPRE.rBG plasmid DNA. cTNT-driven eGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    cTNT
    Tags
    EGFP
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only

  45. pET28a-HaloTag7-SnoopLigase

    Plasmid
    #105627
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7
    Depositor
    Mark Howarth
    Publication
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    HaloTag7-SnoopLigase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    105627-plasmid-map-sequence-id-207217

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  46. pCH1/RAD51o

    Plasmid
    #102562
    Purpose
    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon
    Depositor
    Maria Spies
    Publication
    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11
    Inserts
    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
    E. coli groE operon
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli Expression
    Promoter
    T7
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    102562-plasmid-map-sequence-id-203161

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  47. pAAV.GFAP.eGFP.WPRE.hGH

    Plasmid
    #105549
    Purpose
    AAV expression of EGFP from GFAP promoter
    Depositor
    James M. Wilson
    Has service
    AAV5
    Insert
    eGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    GFAP
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    105549-plasmid-map-sequence-id-202447

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Showing: 7951 - 8000 of 179538 results