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178,225 results

Showing: 7951 - 8000 of 178225 results

  5. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87844-plasmid-map-sequence-id-174620

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  12. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

    Plasmid
    #85225
    Purpose
    Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    John Naughton
    Inserts
    Optimized G Protein
    Enhanced GFP
    TVA (CD320 Chicken)
    Use
    AAV and Cre/Lox
    Promoter
    hSyn
    Available Since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    85225-plasmid-map-sequence-id-170787

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  13. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  14. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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  17. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit
    #1000000073
    Purpose
    An expanded toolkit of integrative vectors for stable gene expression in S. cerevisiae.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73440-plasmid-map-sequence-id-135339

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  19. pHLmMBP-8

    Plasmid
    #72347
    Purpose
    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72347-plasmid-map-sequence-id-133755

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  20. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71657-plasmid-map-sequence-id-422687

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  24. pUV15-pHGFP

    Plasmid
    #70045
    Purpose
    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells
    Depositor
    Sabine Ehrt
    Article
    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.
    Insert
    phGFP
    Use
    Mycobacterium tuberculosis expression vector
    Promoter
    mycobacterial promoter Psmyc
    Available Since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    70045-plasmid-map-sequence-id-488289

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  29. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62200-plasmid-map-sequence-id-395472

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  30. pSNAPf-C1

    Plasmid
    #58186
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    SNAP tag
    Expression
    Mammalian
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    58186-plasmid-map-sequence-id-314094

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  34. mCherry-Farnesyl-5

    Plasmid
    #55045
    Purpose
    Localization: Plasma Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55045-plasmid-map-sequence-id-314088

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  35. mCherry-Alpha-Actinin-19

    Plasmid
    #54975
    Purpose
    Localization: Alpha Actinin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    actinin alpha 1 (ACTN1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    54975-plasmid-map-sequence-id-152016

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  39. CMV-O-GECO1

    Plasmid
    #46025
    Purpose
    Orange intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    O-GECO1
    Expression
    Mammalian
    Mutation
    R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…
    Promoter
    CMV
    Available Since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    46025-plasmid-map-sequence-id-311519

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  47. CMV-EGFP-ssHRP-TM

    Plasmid
    #31156
    Depositor
    Hollis Cline
    Article
    Li et al Front Neural Circuits. 2010 Feb 26;4:6.
    Insert
    ssHRP-TM
    Use
    Membrane targeted hrp (mhrp)
    Expression
    Mammalian
    Mutation
    includes signal sequence and a transmembrane doma…
    Promoter
    CMV promoter including immediate early promoter/e…
    Available Since
    Dec. 14, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 7951 - 8000 of 178225 results