We narrowed to 26,599 results for: vit;

1. pHAGE-FLAG-APEX2-OPTN

   Plasmid

   #175760

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SG3ΔENV RT D185A

   Plasmid

   #145782

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).

   Depositor

   Wesley Sundquist

   Article

   Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

   Insert

   Reverse Transcriptase
   

   Use

   Lentiviral and Retroviral

   Expression

   Mammalian

   Mutation

   Changed Reverse Transcriptase Aspartic Acid 185 t…

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. phGR-SB

   Plasmid

   #133409

   Purpose

   overexpression of a human glucocorticoid receptor in mammalian cells

   Depositor

   Andrew Cato

   Article

   Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

   Insert

   NR3C1 (NR3C1 Human)
   

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-FNLSHiFiNG-P2A-Puro

   Plasmid

   #136903

   Purpose

   Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFiNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Mutation

   R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PM-CaNAR2

   Plasmid

   #64730

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   Lyn-CaNAR2
   

   Tags

   Cerulean3, N-terminal targeting sequence from Lyn…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUB-Cas9

   Plasmid

   #86556

   Purpose

   For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

   Depositor

   Andreas Weber

   Article

   Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   Cas9 is codon optimized for C. reinhardtii

   Promoter

   UBIQUITIN10

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCyPet-Rac1(WT)

   Plasmid

   #22782

   Depositor

   Klaus Hahn

   Article

   Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

   Insert

   Rac1 (wt) (RAC1 Human)
   

   Tags

   CyPet

   Expression

   Mammalian

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

8. GRK2 S670A

   Plasmid

   #45755

   Depositor

   Robert Lefkowitz

   Article

   Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

   Insert

   GRK2 S670A (GRK2 Bovine)
   

   Expression

   Mammalian

   Mutation

   S670A

   Promoter

   CMV

   Available Since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-Tsapphire-StagRFP-SrcAR

   Plasmid

   #173018

   Purpose

   Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Tsapphire-StagRFP-SrcAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-CMV-Galpha-i3 G183S ONE-GO

    Plasmid

    #204338

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-i3 G183S ONE-GO
    

    Use

    Lentiviral

    Mutation

    G183S mutation in Galpha-i3 (renders it RGS insen…

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCAGGS-Hoxc6

    Plasmid

    #45553

    Depositor

    Jeremy Dasen

    Article

    Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

    Insert

    Hoxc6 (Hoxc6 Mouse)
    

    Promoter

    CMV / b-actin

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-mNeonGreen-LEHD-NanoLuc

    Plasmid

    #98289

    Purpose

    caspase-9 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Tags

    His-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSynapsin1-axon-GCaMP6f

    Plasmid

    #112000

    Purpose

    For enriched expression of GCaMP6f in axons

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV1

    Insert

    axon-GCaMP6f
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Promoter

    hSynapsin1

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. ND4.2-Left TALE-G1397-N-DddA6

    Plasmid

    #179680

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (Q1310R S1330I T1380I)_1xUGI_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    Q1310R, S1330I, T1380I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SaCas9 TadDE

    Plasmid

    #193846

    Purpose

    Express TadDE (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE with SaCas9
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-PH1(WT)

    Plasmid

    #86599

    Purpose

    Expresses eGFP-tagged PH(WT) domain of human Akt1

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 PH domain (AKT1 Human)
    

    Expression

    Mammalian

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. mNeongreen-aGBD(anillin)

    Plasmid

    #129633

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    RhoA binding domain of anillin (712-786)
    

    Expression

    Mammalian

    Promoter

    CMVdel

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CANE-LV envelope

    Plasmid

    #86666

    Purpose

    pCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    CANE-LV envelope
    

    Use

    Lentiviral, Mouse Targeting, and Synthetic Biolog…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-mas-KB-1753-Nluc

    Plasmid

    #158603

    Purpose

    Plasmid expressing KB-1753-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCMV4a-SIRT6-H133Y-FLAG

    Plasmid

    #102324

    Purpose

    Human SIRT6 H133Y with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed histidine 133 to tyrosine

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR221_SLC22A1

    Plasmid

    #132034

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A1 (SLC22A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. RCAS-K-ras

    Plasmid

    #11549

    Depositor

    Sandra Orsulic

    Article

    Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

    Insert

    v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras Mouse)
    

    Use

    Retroviral

    Mutation

    G12D

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pTagBFP-Akt1

    Plasmid

    #86594

    Purpose

    Expresses TagBFP-tagged human Akt1(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-His-MinD_MinE

    Plasmid

    #133621

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

    Depositor

    Petra Schwille

    Article

    Loose et al Science. 2008 May 9;320(5877):789-92. do

    Insert

    His-MinD_MinE (minD, minE E. coli)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-Mito-CaNAR2

    Plasmid

    #64731

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2
    

    Tags

    Cerulean3, N-terminal 30 amino acids from DAKAP1,…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. N1-mSin1.2-GFP

    Plasmid

    #72908

    Purpose

    GFP-tagged mSin1 (isoform 2)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS2+ Gal4-GBP2-IRES-GBP7-p65

    Plasmid

    #50020

    Purpose

    Multi-purpose expression of a GFP-dependent transcription factor

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
    

    Use

    Multi-purpose expression

    Promoter

    CMV-immediate early promoter and SP6 promoter

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRSV-CaMKII

    Plasmid

    #45064

    Purpose

    Expression of CaMKII

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    CaM Kinase II (Camk2a Rat)
    

    Expression

    Mammalian

    Promoter

    RSV

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVETL-Cmcs-Flag-Ataxin3Q80

    Plasmid

    #22120

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA-h-mmPtch1-FL

    Plasmid

    #120889

    Purpose

    Expresses wildtype Ptch1 for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-DAKAP-μDpTT

    Plasmid

    #182324

    Purpose

    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    DAKAP-μDronpa-TagRFP-T
    

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLKO.1-sh-mSOX9-2

    Plasmid

    #40645

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSOX9-2 (Sox9 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pcDNA3-mCherry-Rheb(5A)

    Plasmid

    #192439

    Purpose

    Encodes mCherry-tagged Rheb lacking residues 38-42.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb(C181S) (RHEB Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Rheb amino acids 38-42 deleted.

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pVETL-Cmcs-Flag-Ataxin3Q25

    Plasmid

    #22118

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

36. pCAG-GBP1-10gly-Gal4DBD

    Plasmid

    #49438

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-Gal4 DNA binding domain fusion protein
    

    Expression

    Mammalian

    Mutation

    C98S (to increase protein solubility), Q3D

    Promoter

    CAG

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET22b_EcSSB_1

    Plasmid

    #78203

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

    Insert

    ssb (G26C)
    

    Expression

    Bacterial

    Mutation

    G26C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p1071-Lenti-Single loxP

    Plasmid

    #217892

    Purpose

    Lentiviral vector, entry vector for Lentiviral Switch-OVER

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. His-TEV-Aurein1.2_(+36GFP)-LPETG-His

    Plasmid

    #71755

    Purpose

    His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    Aurein1.2_(pos36_GFP)-LPETG-His
    

    Tags

    6XHis, Sortase (LPETG), and TEV Protease

    Expression

    Bacterial

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pILGFPE9

    Plasmid

    #83545

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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