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171,852 results

Showing: 9651 - 9700 of 171852 results

  6. pCS2+ H2B-mTagBFP2

    Plasmid
    #99267
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
    Depositor
    Philipp Keller
    Insert
    mTagBFP2-3XHA
    Tags
    H2B
    Expression
    Mammalian
    Promoter
    SP6
    Available Since
    Sept. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    99267-plasmid-map-sequence-id-192954

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  11. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46569-plasmid-map-sequence-id-364492

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  20. pRS416-PHO5-GFP-Opi1 + terminator

    Plasmid
    #58836
    Purpose
    Expresses GFP-Tagged Opi1 + Terminator in yeast
    Depositor
    Tim Levine
    Insert
    Opi1p (OPI1 Budding Yeast)
    Tags
    GFP
    Expression
    Yeast
    Promoter
    PHO5
    Available Since
    Sept. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107941-plasmid-map-sequence-id-418295

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  25. pGEX6P1-N-Myc

    Plasmid
    #119757
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Myc
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119757-plasmid-map-sequence-id-233668

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  31. pAAV-nEF-Coff/Fon-ChR2-mCherry (AAV8)

    Viral Prep
    #137144-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2-mCherry (#137144). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Flp-dependent expression of ChR2-mCherry for optogenetic activation (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    mCherry (Flp-dependent)
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only

  38. pAAV-nEF-Con/Fon-ChR2-mCherry (AAV8)

    Viral Prep
    #137142-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChR2-mCherry (#137142). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Cre and Flp-dependent expression of channelrhodopsin fused to mCherry for optogenetic activation.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    mCherry (Cre and Flp-dependent)
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  40. AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (AAV1)

    Viral Prep
    #65453-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (#65453). In addition to the viral particles, you will also receive purified AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA plasmid DNA. TRE-driven, Cre and Flp-dependent expression of EGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Promoter
    tetO
    Tags
    EGFP (Cre and Flp-dependent)
    Available Since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only

  42. pFa6a MET25

    Plasmid
    #61913
    Purpose
    Expression of S. cerevisiae MET25 cloned into pFA6a
    Depositor
    Jon Houseley
    Insert
    MET25
    Use
    Yeast genomic targeting
    Available Since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61913-plasmid-map-sequence-id-109819

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  50. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV PHP.eB)

    Viral Prep
    #105530-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 9651 - 9700 of 171852 results