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173,176 results

Showing: 41 - 80 of 173176 results

  3. SSTR5-Tango

    Plasmid
    #66506
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    SSTR5 (SSTR5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV-CAG-SomaGCaMP6f2

    Plasmid
    #158757
    Purpose
    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has Service
    AAV8 and AAV9
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158757-plasmid-map-sequence-id-310662

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  7. pAAV2/6

    Plasmid
    #240485
    Purpose
    AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein
    Depositor
    Meghan Rego, Addgene Research Program
    Insert
    AAV2 Rep and AAV6 Cap
    Use
    AAV
    Expression
    Mammalian
    Available Since
    July 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    240485-plasmid-map-sequence-id-474484

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  11. pCMV-VSV-G

    Plasmid
    #8454
    Purpose
    Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    8454-plasmid-map-sequence-id-404044

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  12. pAAV-hSyn-EGFP (AAV9)

    Viral Prep
    #50465-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only

  13. pRSV-Rev

    Plasmid
    #12253
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    Rev (rev )
    Use
    Lentiviral; Packaging
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12253-plasmid-map-sequence-id-443803

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  15. pMDLg/pRRE

    Plasmid
    #12251
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral; Packaging
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12251-plasmid-map-sequence-id-433915

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  16. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV9)

    Viral Prep
    #44361-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available Since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV5)

    Viral Prep
    #26969-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  21. pAAV-hSyn-mCherry (AAV9)

    Viral Prep
    #114472-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  22. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

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  23. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (AAV5)

    Viral Prep
    #111568-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (#111568). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-mCherry-hPMCA2w/b plasmid DNA. GfaABCD1-driven, expression of mCherry fused plasma membrane calcium pump hPMCA2w/b. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Article
    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)
    Tags
    mCherry
    Available Since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only

  24. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Promoter
    Tight TRE promoter
    Available Since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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  26. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV8)

    Viral Prep
    #105540-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-hSyn-EGFP (AAV8)

    Viral Prep
    #50465-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    Oct. 24, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. pDGM6

    Plasmid
    #110660
    Purpose
    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.
    Depositor
    David Russell
    Article
    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.
    Insert
    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    Use
    AAV
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110660-plasmid-map-sequence-id-337382

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  30. pET-MBP-mSA2

    Plasmid
    #52319
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Tags
    FLAG, His, MBP, and TEV
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52319-plasmid-map-sequence-id-237583

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  33. pSBbi-GP

    Plasmid
    #60511
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60511-plasmid-map-sequence-id-222915

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  39. JD171

    Plasmid
    #229483
    Purpose
    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, VA RNA I, L4-22k/33k
    Use
    AAV and Adenoviral
    Expression
    Mammalian
    Promoter
    CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229483-plasmid-map-sequence-id-455009

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Showing: 41 - 80 of 173176 results