We narrowed to 13,803 results for: cas9 genes

1. phumanCas9 (GB0575)

   Plasmid

   #68206

   Purpose

   Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Cas9 coding region (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAtCas9-dead_1

   Plasmid

   #91165

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A+H840A (dead)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

   Plasmid

   #136659

   Purpose

   Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:TH1477 chimera
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBluescript_VEGFA-TS3_SaCas9_sgRNA2

   Plasmid

   #107330

   Purpose

   U6 driven SaCas9 sgRNA expression for VEGFA site 3

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   VEGFA-TS3 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pICH47742:PtFCP:Cas9YFP

   Plasmid

   #104894

   Purpose

   Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

   Depositor

   Thomas Mock

   Article

   Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

   Insert

   Cas9
   

   Use

   CRISPR; ; golden gate assembly

   Tags

   YFP

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAtCas9-H840A_2

   Plasmid

   #91163

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_H840A (nickase)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   H840A

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KA2706_eSpCas9-PGKPacdtkCh

   Plasmid

   #124204

   Purpose

   Expression of eSpCas9(1.1) and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. UAS-u(XL)Cas9

   Plasmid

   #127386

   Purpose

   Expresses Cas9 at very low level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(XL)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAST

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pdCas9- AsiA_m1.1

   Plasmid

   #158064

   Purpose

   Expressing evolved CasTA of dCas9 fused AsiA variant 1.1

   Depositor

   Harris Wang

   Article

   Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

   Insert

   dCas9-AsiA_m1.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBLO 36.02_ProCas9TEV

    Plasmid

    #121624

    Purpose

    TetR_tetR/tetA promoters_bCas9-CP199-TEVlinker-2xflag_CM_P15A

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p15A

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHdCas9-mammalian

    Plasmid

    #138415

    Purpose

    Expression of Himar-dCas9 in mammalian cells

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAtCas9-dead_3

    Plasmid

    #91167

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A+H840A (dead)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBluescript_VEGFA-TS3_SpCas9

    Plasmid

    #107328

    Purpose

    U6 driven SpCas9 sgRNA expression for VEGFA site 3

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    VEGFA-TS3 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBluescript_VEGFA-TS3_NmCas9

    Plasmid

    #107329

    Purpose

    U6 driven NmCas9 sgRNA expression for VEGFA site 3

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    VEGFA-TS3 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330A_dCas9-1x5

    Plasmid

    #63599

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBLO43.3_human ProCas9Flavi

    Plasmid

    #121629

    Purpose

    U6-sgRNAdest_CMV-intron_hProCas9Flavi_T2A Mcherry_AmpR_ColE1

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEM-Cas9noSsrA

    Plasmid

    #102285

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    removed ssrA tag

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330A_dCas9-1x3

    Plasmid

    #63597

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTaCas9-H840A_5

    Plasmid

    #91172

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_H840A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTaCas9-D10A_6

    Plasmid

    #91171

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAtCas9-D10A_3

    Plasmid

    #91161

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pD10AH840AhCas9 (GB1041)

    Plasmid

    #68207

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKDcas9 71 cerulean

    Plasmid

    #230954

    Purpose

    aTc inducible knockdown of FliZ and CRP

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKDcas9 13 cerulean

    Plasmid

    #230955

    Purpose

    aTc inducible knockdown of CRP and HNS

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKDcas9 24 cerulean

    Plasmid

    #230956

    Purpose

    aTc inducible knockdown of Fis and mcaS

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKDcas9 35 cerulean

    Plasmid

    #230957

    Purpose

    aTc inducible knockdown of HNS and CsgD

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKDcas9 46 cerulean

    Plasmid

    #230958

    Purpose

    aTc inducible knockdown of mcaS and FlhDC

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKDcas9 57 cerulean

    Plasmid

    #230959

    Purpose

    aTc inducible knockdown of CsgD and FliZ

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKDcas9 72 cerulean

    Plasmid

    #230961

    Purpose

    aTc inducible knockdown of FliZ and Fis

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKDcas9 14 cerulean

    Plasmid

    #230962

    Purpose

    aTc inducible knockdown of CRP and mcaS

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKDcas9 47 cerulean

    Plasmid

    #230965

    Purpose

    aTc inducible knockdown of mcaS and FliZ

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKDcas9 51 cerulean

    Plasmid

    #230966

    Purpose

    aTc inducible knockdown of CsgD and CRP

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKDcas9 62 cerulean

    Plasmid

    #230967

    Purpose

    aTc inducible knockdown of FlhDC and Fis

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKDcas9 73 cerulean

    Plasmid

    #230968

    Purpose

    aTc inducible knockdown of FliZ and HNS

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKDcas9 23 cerulean

    Plasmid

    #230949

    Purpose

    aTc inducible knockdown of Fis and HNS

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKDcas9 45 cerulean

    Plasmid

    #230951

    Purpose

    aTc inducible knockdown of mcaS and CsgD

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKDcas9 56 cerulean

    Plasmid

    #230952

    Purpose

    aTc inducible knockdown of CsgD and FlhDC

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKDcas9 67 cerulean

    Plasmid

    #230953

    Purpose

    aTc inducible knockdown of FlhDC and FliZ

    Depositor

    Andrew Griffiths

    Article

    Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    additional tetO

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SIN40C.SFFV.dCas9-KRAB.P2A.mNeon

    Plasmid

    #170482

    Purpose

    Lentiviral dSpCas9-KRAB (CRISPRi) delivery, mNeon coexpression

    Depositor

    Dirk Heckl

    Article

    Ng et al iScience. 2023 Sep 7;26(10):107844. doi:

    Inserts

    dCas9-KRAB
    SFFV
    P2A-mNeonGreen
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. iPE-WT-Cas9

    Plasmid

    #239379

    Purpose

    For inverse prime editor using WTCas9 in HEK294T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

    Insert

    Cas9, M-MLV RT
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge