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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 23,588 results for: CHI

Showing: 10901 - 10950 of 23588 results

  7. HpMscS

    Plasmid
    #78310
    Purpose
    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag
    Depositor
    Douglas Rees
    Article
    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:
    Insert
    Mechanosensitive channel of small conductance
    Use
    Tags
    6x His Tag and FLAG Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    78310-plasmid-map-sequence-id-151301

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  8. pAAV-CaMKIIa-ChRger3-TS-EYFP

    Plasmid
    #127241
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger3-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIa
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127241-plasmid-map-sequence-id-247096

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  9. pBICI_2_strong

    Plasmid
    #155168
    Purpose
    see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155168-plasmid-map-sequence-id-310553

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  11. pGBW-m4046931

    Plasmid
    #145706
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145706-plasmid-map-sequence-id-307566

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  19. pCarCBA

    Plasmid
    #117754
    Purpose
    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117754-plasmid-map-sequence-id-229023

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  21. Nano-lantern-H2B/pcDNA3

    Plasmid
    #51971
    Purpose
    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B
    Depositor
    Takeharu Nagai
    Article
    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc
    Insert
    Nano-lantern
    Use
    Tags
    human H2B
    Expression
    Mammalian
    Mutation
    S257G in Rluc8
    Promoter
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pUE1-TSP-ORF68 K395A/K396A

    Plasmid
    #162656
    Purpose
    Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1
    Insert
    ORF68
    Use
    Tags
    N-terminal 2xStrep-Tag II and HRV 3C site
    Expression
    Mammalian
    Mutation
    Amino acids K395 and K396 mutated to alanine
    Promoter
    CMV
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162656-plasmid-map-sequence-id-320674

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  30. pAAV-CaMKIIa-ChRger1-TS-EYFP

    Plasmid
    #127244
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIa
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127244-plasmid-map-sequence-id-247231

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  37. ERroGFP_iE_C204Q_pCDNA3

    Plasmid
    #48629
    Purpose
    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERroGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    insertion E147B, C204Q
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48629-plasmid-map-sequence-id-70377

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  38. pGBW-m4046863

    Plasmid
    #145682
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145682-plasmid-map-sequence-id-298370

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  42. ERroGFP_C147S_pCDNA3

    Plasmid
    #48630
    Purpose
    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERroGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48630-plasmid-map-sequence-id-70378

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  50. ERrefGFP1_pCDNA3

    Plasmid
    #48635
    Purpose
    Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    ERrefGFP1
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    C147S,C204Q
    Promoter
    Available since
    Oct. 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    48635-plasmid-map-sequence-id-70401

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Showing: 10901 - 10950 of 23588 results