We narrowed to 16,609 results for: IGH@

1. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-30a(+)-N-SH2

   Plasmid

   #111274

   Purpose

   express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
   

   Use

   Tags

   HHHHHHENLYFQG

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGS_N_Strep

   Plasmid

   #101651

   Purpose

   Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, KpnI linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGS_C_Strep

   Plasmid

   #101652

   Purpose

   Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, MCS linker, Twin-Strep tag
   

   Use

   Tags

   Twin-Strep tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMflCT-o4

   Plasmid

   #101313

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   cat resistance gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMflT-o3

   Plasmid

   #101311

   Purpose

   Contains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Insert

   oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLhT-pHG165a V52A

   Plasmid

   #90050

   Purpose

   Constitutive expression of a chimeric LacI:TreR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhT/V52A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V52A

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLhA-pHG165a Q55A

    Plasmid

    #90052

    Purpose

    Constitutive expression of a chimeric LacI:AscG transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhA/Q55A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q55A

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LLhC-pHG165a Q55I

    Plasmid

    #90053

    Purpose

    Constitutive expression of a chimeric LacI:CytR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhC/Q55I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q55I

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RGD-I27-CC

    Plasmid

    #85393

    Purpose

    MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling

    Depositor

    Khalid Salaita

    Article

    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

    Insert

    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL2-MCT2Prom2.5

    Plasmid

    #87351

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    2.5 kbp proximal promoter of human MCT2 (nested d…

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL2-MCT2Prom1.1

    Plasmid

    #87352

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    1.1 kbp proximal promoter of human MCT2 (nested d…

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL2-MCT2Prom3.2

    Plasmid

    #87349

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    3.2 kbp proximal promoter of MCT2 (nested deletio…

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCIneoRL-BCL2 3'UTR-mir200c-mut

    Plasmid

    #84598

    Purpose

    Renilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    BCL2 3'UTR (UTS2R Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on BCL2 3'…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCIneoRL-TOB1 3'UTR-mir200c-mut

    Plasmid

    #84590

    Purpose

    Renilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    TOB1 3'UTR (UTS2R Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on TOB1 3'…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCIneoRL-RanBP93'UTR-mir200c-mut

    Plasmid

    #84592

    Purpose

    Renilla Luciferase reporter assay for RANBP9 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    RANBP9 3'UTR (RANBP9 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on RANBP9 3…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pNIA-CEN-FLAG-LINXa4-Bre1

    Plasmid

    #73617

    Purpose

    Control of the E3 ligase Bre1 with LINXa in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa4-Bre1dNLS
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHL836

    Plasmid

    #62827

    Purpose

    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    gfp
    ipex leader
    hfq leader
    

    Use

    Synthetic Biology

    Tags

    ompC leader and st7 RBS

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pHL1954

    Plasmid

    #62831

    Purpose

    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    M8::ryhB leader
    hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::sodB leader and st7 RBS

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind, pLlacO-1, and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pHL1969

    Plasmid

    #62832

    Purpose

    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    M8::ryhB leader
    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::sodB leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind, pLlacO-1, and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pHL2107

    Plasmid

    #62841

    Purpose

    Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    m8::micC leader
    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::ompC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind, pLlacO-1, and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

24. mCardinal-Cx26-7

    Plasmid

    #56156

    Purpose

    Localization: Gap Junctions, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Cx26
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

25. mCardinal-CAF1-C-10

    Plasmid

    #56153

    Purpose

    Localization: Chromatin Assembly Factor 1, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    CAF1
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

26. tdminiSOG-Tubulin-6

    Plasmid

    #57808

    Purpose

    Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    tdminiSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

27. PA-mCherry-miniSOG-Vinculin-23

    Plasmid

    #57801

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

28. miniSOG-Vinculin-23

    Plasmid

    #57780

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

29. mCerulean3-Keratin-17

    Plasmid

    #55424

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

30. mCerulean3-Lasp1-N-10

    Plasmid

    #55426

    Purpose

    Localization: Focal Adhesions, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Lasp1 (LASP1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    A209V

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

31. mCerulean3-CDC42-C-10

    Plasmid

    #55406

    Purpose

    Localization: Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    CDC42 (CDC42 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

32. mCherry-miniSOG-Zyxin-6

    Plasmid

    #55101

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Zyxin (ZYX Human)
    

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pMM2-hPMCA4bct120

    Plasmid

    #47760

    Purpose

    mammalian expression of human PMCA4 with C-terminal 120aa deletion

    Depositor

    John Penniston, Emanuel Strehler

    Article

    Enyedi et al J Biol Chem. 1993 May 15;268(14):10621-6

    Insert

    PMCA4bct120 (ATP2B4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of C-terminal 120 aa (removes calmodulin…

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

34. AB.pCCL.sin.cPPT.U6.miR-32-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46608

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-32 Decoy (MIR32, Mir32 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

35. AB.pCCL.sin.cPPT.U6.miR-759-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46634

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-759 Decoy (Mir759, MIR759 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

36. AB.pCCL.sin.cPPT.U6.miR-761-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46635

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-761 Decoy (Mir761, MIR761 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

37. AB.pCCL.sin.cPPT.U6.miR-192-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46597

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-192 Decoy (MIR192, Mir192 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

38. GoldyTALEN-moesina TAL1

    Plasmid

    #41831

    Depositor

    Jeffrey Essner

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    moesin a (msna Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GoldyTALEN-moesina TAL2

    Plasmid

    #41832

    Depositor

    Jeffrey Essner

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    moesin a (msna Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pIRES:hTRAAK(W262S)

    Plasmid

    #133078

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pIRES:hTRAAK(G124I)

    Plasmid

    #133079

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G124I

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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42. pPICZ:hTRAAK(W262S)

    Plasmid

    #130670

    Purpose

    P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    KCNK4 (KCNK4 Human)
    

    Use

    Tags

    SNS- 3C_cleavage_site-TAAA-GFP

    Expression

    Yeast

    Mutation

    N104Q, N108Q, W262S

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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43. pAAV-hSyn-mScarlet (AAV1)

    Viral Prep

    #131001-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSyn-mScarlet (#131001). In addition to the viral particles, you will also receive purified pAAV-hSyn-mScarlet plasmid DNA. Synapsin-driven mScarlet control vector. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    Syn

    Tags

    mScarlet

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

44. pAAV-hSyn-mScarlet (AAV8)

    Viral Prep

    #131001-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-mScarlet (#131001). In addition to the viral particles, you will also receive purified pAAV-hSyn-mScarlet plasmid DNA. Synapsin-driven mScarlet control vector. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    Syn

    Tags

    mScarlet

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

45. pAAV-CaMKIIa-mScarlet (AAV8)

    Viral Prep

    #131000-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-mScarlet (#131000). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mScarlet plasmid DNA. CamKIIa-driven expression of mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    CaMKII

    Tags

    mScarlet

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

46. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pHR-HNRNPA1C-mCh-Cry2WT

    Plasmid

    #101226

    Purpose

    hnRNPA1(186-320) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    hnRNPA1(186-320) (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. p3HA-hMIB1

    Plasmid

    #33317

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    MIB1 (MIB1 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLenti-TripleColour-StAR

    Plasmid

    #222695

    Purpose

    Competition assay

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP, miRFP703, dTomato

    Expression

    Mammalian

    Mutation

    PGK, TagBFP, dTomato without BsmBI cutsite

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLVX-EF1a-tetOn-IRES-G418 (EtO)

    Plasmid

    #84776

    Purpose

    Second generation lenti tetracycline operator (on)

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Insert

    TET OPERATOR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTIGHT

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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