167,729 results

1. pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92392

   Purpose

   produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   Human Synapsin

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag-β-Lactamase-SpyCatcher

   Plasmid

   #52656

   Purpose

   Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

   Depositor

   Mark Howarth

   Article

   Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

   Insert

   TEM-1 β-Lactamase
   

   Tags

   SpyCatcher and SpyTag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-5FLAG-KanMX6

   Plasmid

   #15983

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Type

   Empty backbone

   Tags

   5xFlag

   Expression

   Bacterial

   Available Since

   Oct. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1-GfaABC1D-lck-jGCaMP8s (AAV9)

   Viral Prep

   #176761-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8s (#176761). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8s plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   GfaABC1D

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

5. opto-Cdc42

   Plasmid

   #174510

   Purpose

   Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   opto-Cdc42-mCherry
   

   Tags

   3xFLAG and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOLA-Gent-EM7-Erv1p-DsbC

   Plasmid

   #202485

   Purpose

   Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
   

   Expression

   Bacterial

   Promoter

   EM7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. E. coli MEV20

   Bacterial Strain

   #197113

   Purpose

   E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Bacterial Resistance

   None

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

8. KJ740(DE3)

   Bacterial Strain

   #179486

   Purpose

   KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Bacterial Resistance

   Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)

   Species

   Escherichia coli

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

9. pAAV:cTNT::Luciferase

   Plasmid

   #69915

   Purpose

   Used to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.

   Depositor

   William Pu

   Article

   Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

   Insert

   Firefly luciferase
   

   Use

   AAV

   Promoter

   Chicken cardiac troponin T

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK463 (BSD-P2A-mAB)

    Plasmid

    #214389

    Purpose

    mAID-BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-mAID-BromoTag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. hPGK-rtTA3

    Plasmid

    #195883

    Purpose

    Lentiviral construct of hPGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    rtTA3
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Anti-CASPR2 [K67/25R] (Recombinant Antibody)

    Antibody

    #184177-rAb

    Purpose

    Anti-CASPR2 (Human) recombinant mouse monoclonal antibody

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Recommended Applications

    Immunohistochemistry and Western Blot

    Reactivity

    Human, Mouse, and Rat

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Available to purchase

    Available Since

    June 13, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

13. Halo-Sec23A

    Plasmid

    #166892

    Purpose

    mammalian expression of Sec23A tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    SEC23A (SEC23A Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. wt dynamin 2 pEGFP

    Plasmid

    #34686

    Depositor

    Sandra Schmid

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406G, V664A, H692R and Q758R

    Promoter

    CMV

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-KD LKB1

    Plasmid

    #8591

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K78I: lysine 78 mutated to isoleucine; kinase dead

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-Rab5a-7

    Plasmid

    #55126

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. siRNA-resistant SigmaR1-mNeonGreen R119A F191A

    Plasmid

    #226574

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    R119A, F191A

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-deadMalibu-mScarlet

    Plasmid

    #229555

    Purpose

    Fluorescent reporter for malonyl-CoA that does not bind malonyl-CoA (control)

    Depositor

    Danielle Schmitt

    Article

    Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

    Insert

    Malibu-mScarlet
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Roxed-Cre

    Plasmid

    #51273

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human Parkin (1-465)

    Plasmid

    #110758

    Purpose

    Bacterial expression of human Parkin

    Depositor

    David Komander

    Article

    Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

    Insert

    PRKN (PRKN Human)
    

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSR33.4r

    Plasmid

    #63198

    Purpose

    Expresses Cph8, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    Cph8
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHSV-TK

    Plasmid

    #165801

    Purpose

    Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    HSV-TK
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

    Plasmid

    #184632

    Purpose

    Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad67 (Gad1 Mouse)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. negMaLionB

    Plasmid

    #113911

    Purpose

    negative control for Blue ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    negMaLionB
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

    Plasmid

    #132404

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pECE

    Plasmid

    #26453

    Depositor

    William Rutter

    Article

    Ellis et al Cell. 1986 Jun 6;45(5):721-32.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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27. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid

    #118156

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pSynSRE-T-Luc

    Plasmid

    #60444

    Purpose

    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

    Depositor

    Timothy Osborne

    Article

    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

    Insert

    HMG-CoA synthase promoter (-324/-225)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. LV anti-FITC-LaG16-Y186A-T2A-BFP

    Plasmid

    #194932

    Purpose

    LV construct encoding Y186A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-Casp3-myc

    Plasmid

    #11813

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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31. pcDNA-Wnt7B

    Plasmid

    #35915

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt7B (Wnt7b Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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32. M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

    Plasmid

    #122668

    Purpose

    C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    HNRNPA1 (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-SspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 186-230

    Promoter

    SFFV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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33. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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34. pAAV_hSyn1-SIO-stGtACR1-FusionRed

    Plasmid

    #105678

    Purpose

    Expresses a soma-targeted GtACR1 in Cre-expressing neurons

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV5

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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35. pDragon-p21

    Plasmid

    #155016

    Purpose

    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activity

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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36. EGFP-m-Paxillin

    Plasmid

    #80023

    Purpose

    Mammalian expression of mouse EGFP-Paxillin

    Depositor

    Chinten James Lim

    Insert

    Paxillin (Pxn Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TFORF2207

    Plasmid

    #143354

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREB3L2 (CREB3L2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. UbcH5A

    Plasmid

    #61081

    Purpose

    express a human E2

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    UbcH5A (UBE2D1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pLCAG EGFP

    Plasmid

    #14857

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2002 Feb 19. 9

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pHR SFFVp Phy-mCherry-CAAX

    Plasmid

    #50839

    Purpose

    Optogenetic PI3K activation system; membrane component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

    Insert

    PhyB 1-908
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    mCherry

    Promoter

    SFFV

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge