188,398 results
-
Plasmid#31766DepositorInsertHA-YFP-FKBP(DD) (FKBP1A Human)
UseRetroviralTagsHA and YFPExpressionMutationE31G, F36V, R71G and K105E on FKBP12PromoterLTRAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-FKBP(DD)
Plasmid#31766DepositorInsertHA-YFP-FKBP(DD) (FKBP1A Human)
UseRetroviralTagsHA and YFPExpressionMutationE31G, F36V, R71G and K105E on FKBP12PromoterLTRAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO mCherry (AAV2)
Viral Prep#114471-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Flp-dependent)Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6f
Plasmid#207856PurposeMammalian expression of PE6f prime editorDepositorInsertPE6f
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human Kinase Domain-Focused CRISPR Knockout Library
Pooled Library#117725PurposegRNAs targeting the kinase domain of human protein kinasesDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Luc2=tdT
Plasmid#32904PurposeExpresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.DepositorInsertsFirefly Luciferase
tandem Tomato Red Fluorescent Protein
UseTagstdTomato red fluorescent proteinExpressionMammalianMutationPromoterCMV-IE and n/aAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Luc2=tdT
Plasmid#32904PurposeExpresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.DepositorInsertsFirefly Luciferase
tandem Tomato Red Fluorescent Protein
UseTagstdTomato red fluorescent proteinExpressionMammalianMutationPromoterCMV-IE and n/aAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ
Plasmid#178320PurposeMultiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.DepositorInsertsHRE RedFirefly reporter
P53 FLuc reporter
AP-1 Renilla reporter
FOXO NLuc Reporter
SMAD GrRenilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A
Plasmid#207953Purposenuclease inactive control for TRF1FokI WTDepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFlag, DD, ER, mCherry and FokI (D450A)ExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP
Plasmid#188767PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin
Plasmid#187953PurposeFKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-SpdCas9-KRAB-tagBFP
UseCRISPR and Synthetic BiologyTagsGGSExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Puro.Cre empty vector
Plasmid#17408PurposeLentiviral expression of Cre from the mouse PGK promoterDepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromotermouse PGKAvailable sinceMarch 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
Puro.Cre empty vector
Plasmid#17408PurposeLentiviral expression of Cre from the mouse PGK promoterDepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromotermouse PGKAvailable sinceMarch 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-JunDN
Plasmid#40350DepositorInsertJun dominant negative (JUN Human)
UseRetroviralTagsHA and IRES eGFPExpressionMammalianMutationfirst 122 amino acids deletedPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-JunDN
Plasmid#40350DepositorInsertJun dominant negative (JUN Human)
UseRetroviralTagsHA and IRES eGFPExpressionMammalianMutationfirst 122 amino acids deletedPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCh-Cry2WT
Plasmid#101221PurposeBlue-light dependent optogenetic protein Cry2DepositorInsertCry2PHR
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF2523
Plasmid#142523PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNRF1 (NRF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII0.4.eGFP.WPRE.rBG
Plasmid#105541PurposeAAV expression of EGFP from CamKII promoterDepositorHas ServiceAAV1 and AAV5InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterCamkIIAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-TfR-mApple
Plasmid#179384PurposeLentiviral vector for expression of Human Transferrin receptor-1 fused mAppleDepositorInsertTransferrin receptor 1 - mApple (TFRC Human, Synthetic)
UseLentiviralTagsmAppleExpressionMammalianMutationPromoterEF1Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
S1PR2-Tango
Plasmid#66497PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertS1PR2 (S1PR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-Parkin
Plasmid#17612DepositorInsertParkin (PRKN Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-Parkin
Plasmid#17612DepositorInsertParkin (PRKN Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10873
Plasmid#183961PurposeU6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPRDepositorInsertsHyperdCas12a
poly-crRNA array targeting Sox2-Klf4-Oct4
UseCRISPRTagsHAExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAG and U6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-TC
Plasmid#183754PurposeAAVS1 targeting construct with the Bxb1 landing pad cassette.DepositorInsertloxP-eGFP-attP-Bsd-lox257
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MGAT-SEP-mRuby3
Plasmid#200937Purposecis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasso
Plasmid#207530PurposeConditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgDepositorInsertcytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSFV3-lacZ
Plasmid#92074PurposeThis is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.DepositorInsertlacZ
UseLacz expression plasmid, for run-off transcriptio…TagsExpressionMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR
Plasmid#224435PurposeAAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmScarlet-KASH
U6-gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-rM3D(Gs)
Plasmid#45549PurposeExpresses rM3D (Gs) neuronal activatorDepositorInsertrM3D (Gs)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only