164,782 results
-
-
qTAG-C-mScarlet-EFS-Blast
Plasmid#227260PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR8-Tango
Plasmid#66245PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCCR8 (CCR8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI3_G-alpha
Plasmid#110014PurposeProtein expression and purification of GNAI3_G-alphaDepositorInsertGNAI3_G-alpha (GNAI3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL0_1 [RK2 ORI]
Plasmid#198918PurposeLevel 0 partDepositorInsertRK2 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMito-mCherry-FRB_IRES_FKBP(III)-GBPen
Plasmid#128268PurposeBicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).DepositorInsertsMitoTrap
FKBP(III)-GBPen
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer10b-HA-KRAS G12V CO SR
Plasmid#164928PurposeExpresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequenceDepositorInsertKRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
UseLentiviralTagsHA tagExpressionMutationGlycine 12 changed to valine; 9 Valine codons are…PromoterUbc promoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-CREB (S133A)
Plasmid#137002PurposemEGFP-CREB donor for G-CREB with S133A mutationDepositorInsertCREB1 (Creb1 Rat)
UseTagsmEGFPExpressionMammalianMutationChanges Serine 133 into AlaninePromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f
Plasmid#182548PurposeExpresses GCaMP6f on the cytosolic side of the ER (P-450site)DepositorInsertER localized P450
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-RUNX3-T2A-PuroR
Plasmid#162349PurposeLentiviral expression of RUNX3 under the control of the TetON promoterDepositorInsertRUNX3 (RUNX3 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
p7140 pHAGE-P-CMVt-N-HA-GAW-EME1
Plasmid#100150PurposeExpresses EME1DepositorInsertEME1 (EME1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-Mango II x 24
Plasmid#127587PurposeExpresses the mCherry-Mango II x 24 array from a TetON responsive promoterDepositorInsertmCherry-Mango II x24
UseTagsmCherryExpressionMammalianMutationPromoterpTRE Tet ONAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
XE118 CAMKII-T286D-CS2+
Plasmid#16736DepositorInsertCAMKII-T286D (Camk2a Rat)
UseXenopus expressionTagsExpressionMutationthreonine 286 mutated to aspartatePromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-CAMKIIalpha-T286A
Plasmid#21220DepositorInsertCAMKIIalpha-T286A (Camk2a Rat)
UseTagsGFPExpressionMammalianMutationT286A (Lab plasmid KS 0003).PromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PIM1
Plasmid#23692DepositorInsertPIM1 (PIM1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSuMMVp
Plasmid#118970PurposeExpresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSuMMVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cSuMMVp
Plasmid#119182PurposeExpresses C-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cSuMMVp in fusion with FKBP
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0091
Plasmid#181955PurposeBeta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/ClucDepositorInsert7xFOP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-CAMKIIalpha-K42R
Plasmid#21221DepositorInsertCAMKIIalpha-K42R (Camk2a Rat)
UseTagsGFPExpressionMammalianMutationK42R (Lab plasmid KS 0007).PromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9
Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
UseTagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA-DP-1
Plasmid#133705PurposeExpression of HA-tagged TFDP1.DepositorInsertTFDP1 (TFDP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLibacceptorV2
Plasmid#106250PurposeRecombination-mediated cassette exchange with target genomic lociDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHM092
Plasmid#225526PurposeEncodes rtTA under AR8 promoter, and constitutive BFPDepositorInsertsrtTA
BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHM093
Plasmid#225525PurposeEncodes rtTA under IBRE4 promoter, and constitutive BFPDepositorInsertsrtTA
BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHM039
Plasmid#225523PurposeEncodes Wnt-responsive rtTA and constitutive BFPDepositorInsertsrtTA
BFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
PEP101E
Plasmid#97387Purposebinary construct of cytosolic sfGFP1-10 for plantDepositorInsertsfGFP1-10OPT
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
MoClo Yeast Toolkit
Plasmid Kit#1000000061Purpose96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettesDepositorAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Xph15-GCaMP6f
Plasmid#135538PurposeEncodes a specific PSD-95 binder (Xph15) fused to GCaMP6fDepositorInsertXph15
UseTagsGCaMP6fExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Raptor Halo
Plasmid#112754Purposeexpress Raptor-HaloDepositorInsertRaptor-Halo (RPTOR Human)
UseTagsHaloExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(AtUBI10)
Plasmid#202016PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
UseTagsGB1 and sfGFPExpressionPlantMutationPromoterAtUBI10Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP249 pGK-dCas9-DNMT3a V3
Plasmid#100938PurposedCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1aDepositorInsertdCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
UseCRISPR and LentiviralTags3xHA and 3xTy1ExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSN840
Plasmid#184832PurposeExpresses human KIF5A(∆exon27) and human KLC1 in insect cellsDepositorUseCre/LoxTagsHis-FLAG and mScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterp10 and polyhedrinAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
enOgeuIscB with ωRNA-v2
Plasmid#220939PurposeCMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloingDepositorInsertsCMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT4/HB
Plasmid#108352PurposeOptimized Sleeping Beauty Transposon Vector with MCSDepositorTypeEmpty backboneUseTransposonTagsExpressionMutationPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-dLight1.3b (AAV9)
Viral Prep#135762-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-syn-dLight1.3b (#135762). In addition to the viral particles, you will also receive purified pAAV-syn-dLight1.3b plasmid DNA. Syn-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF2963
Plasmid#144439PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR2C2 (NR2C2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-Puro (pRW1441)
Plasmid#216217PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only
