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167,913 results

Showing: 1841 - 1880 of 167913 results

  3. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105841-plasmid-map-sequence-id-490369

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  15. AAV-mDlx-jGCaMP8m-WPRE (AAV9)

    Viral Prep
    #176754-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-mDlx-jGCaMP8m-WPRE (#176754). In addition to the viral particles, you will also receive purified AAV-mDlx-jGCaMP8m-WPRE plasmid DNA. mDlx enhancer-driven expression of calcium sensor GCaMP8m in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    mDlx
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  16. biGBac Kit

    Plasmid Kit
    #1000000088
    Purpose
    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.
    Depositor
    Jan-Michael Peters
    Available Since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  18. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

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  21. pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (AAV8)

    Viral Prep
    #60658-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (#60658). In addition to the viral particles, you will also receive purified pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato plasmid DNA. CAG-driven, Flp-dependent expression of the voltage-gated Na+ channel mNaChBac-T2A-tdTomato. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Massimo Scanziani
    Article
    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d
    Promoter
    EF1α
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  23. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV5)

    Viral Prep
    #100845-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and TRE3G
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  27. pSMAL-CellTag-V1 (Concentrated Lentiviral Prep)

    Viral Prep
    #115643-LVC
    Purpose
    Ready-to-use Concentrated Lentiviral Prep particles produced from pSMAL-CellTag-V1 (#115643). In addition to the viral particles, you will also receive purified pSMAL-CellTag-V1 plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics.&lt;/p&gt;</p>
    Depositor
    Samantha Morris
    Article
    Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:
    Promoter
    Minimal CMV
    Tags
    EGFP
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only

  28. Human ubiquitination-related proteins CRISPR KO library

    Pooled Library
    #174592
    Purpose
    CRISPR Cas9 KO library targets 660 ubiquitination-related proteins, including all major E1, E2, E3 and deubiquitinating enzymes, as well as other ubiquitination-related proteins.
    Depositor
    Juan Bonifacino
    Article
    Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available Since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only

  34. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100852-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 (#100852). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent jRGECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    CAG
    Available Since
    July 23, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1841 - 1880 of 167913 results