Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

167,924 results

Showing: 1961 - 2000 of 167924 results

  2. pAAV-Ef1a-fDIO mCherry (AAV1)

    Viral Prep
    #114471-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available Since
    Dec. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV-Ef1a-mCherry (AAV8)

    Viral Prep
    #114470-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV-FLEX-GFP

    Plasmid
    #28304
    Purpose
    Cre-dependent AAV-mediated expression of GFP under the CAG promoter
    Depositor
    Edward Boyden
    Insert
    GFP
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available Since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    28304-plasmid-map-sequence-id-454424

    Enlarge

  6. pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV1)

    Viral Prep
    #83897-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  8. pEGFP Vinculin

    Plasmid
    #50513
    Depositor
    Kenneth Yamada
    Insert
    Vinculin (VCL Chicken)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50513-plasmid-map-sequence-id-170487

    Enlarge

  14. pAAV-FLEX-tdTomato (AAV1)

    Viral Prep
    #28306-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-GFAP-hM3D(Gq)-mCherry (AAV5)

    Viral Prep
    #50478-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP-hM3D(Gq)-mCherry (#50478). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM3D(Gq)-mCherry plasmid DNA. GFAP-driven hM4D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    GFAP
    Tags
    mCherry
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid
    #74939
    Purpose
    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    Ubiquitin
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available Since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    74939-plasmid-map-sequence-id-151347

    Enlarge

  21. pINDUCER dCas9-TET1CDMut

    Plasmid
    #101920
    Purpose
    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
    Depositor
    Danwei Huangfu
    Article
    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10
    Insert
    dCas9-TET1CD Mut
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Mutated TET1 catalytic domain
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101920-plasmid-map-sequence-id-194554

    Enlarge

  22. dTAG-IRF8

    Plasmid
    #190620
    Purpose
    Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation
    Depositor
    Junwei Shi
    Article
    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1
    Insert
    IRF8 (IRF8 Human)
    Use
    Lentiviral
    Tags
    FKBP12_G36V
    Expression
    Mammalian
    Mutation
    synonymous mutation for sgRNA resistance for sgIR…
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    190620-plasmid-map-sequence-id-372992

    Enlarge

  26. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

    Plasmid
    #214265
    Purpose
    Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.
    Depositor
    D. James Surmeier
    Insert
    Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
    Use
    AAV
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Synapsin
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214265-plasmid-map-sequence-id-429396

    Enlarge

  27. TFORF2465

    Plasmid
    #142946
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    IRF6 (IRF6 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142946-plasmid-map-sequence-id-391567

    Enlarge

  29. G13-CASE

    Plasmid
    #168127
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F125/D126 within GNA13
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168127-plasmid-map-sequence-id-332872

    Enlarge

  31. pLVX-puro-cOVA-IRES-BFP

    Plasmid
    #135074
    Purpose
    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.
    Depositor
    Maria Castro
    Insert
    cOVA-IRES-BFP (OVAL Chicken)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    135074-plasmid-map-sequence-id-266791

    Enlarge

  32. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid
    #177672
    Purpose
    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    nEF
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177672-plasmid-map-sequence-id-348533

    Enlarge

  36. PB-UniSAM

    Plasmid
    #99866
    Purpose
    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0
    Depositor
    Lesley Forrester
    Article
    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.
    Insert
    UniSAM-mCherry + U6-gRNA2.0
    Use
    CRISPR and Synthetic Biology; Dcas9-sam activation
    Expression
    Mammalian
    Mutation
    BbsI sites in CDS were ablated by consensus mutat…
    Promoter
    EF1a
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99866-plasmid-map-sequence-id-192399

    Enlarge

Showing: 1961 - 2000 of 167924 results