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163,546 results

Showing: 3251 - 3300 of 163546 results

  1. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

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  5. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  6. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid
    #201917
    Purpose
    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201917-plasmid-map-sequence-id-403313

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  12. AAV-hSyn-Soma-jGCaMP8m (AAV9)

    Viral Prep
    #169257-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8m (#169257). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8m plasmid DNA. Syn-driven expression of soma-targeted calcium sensor jGCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Promoter
    Tags
    None
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  18. Mito-GA

    Plasmid
    #209865
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Use
    Tags
    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209865-plasmid-map-sequence-id-415469

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  19. tet-pLKO-sgRNA-puro

    Plasmid
    #104321
    Purpose
    3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.
    Depositor
    Nathanael Gray
    Article
    Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    104321-plasmid-map-sequence-id-323839

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  23. pZac2.1 gfaABC1D-lck-GCaMP6f (AAV5)

    Viral Prep
    #52924-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pZac2.1 gfaABC1D-lck-GCaMP6f (#52924). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-lck-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) lck-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Promoter
    Tags
    None
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only

  28. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid
    #167288
    Purpose
    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.
    Depositor
    Agnel Sfeir
    Article
    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:
    Insert
    DDX58 (RIGI Human)
    Use
    Lentiviral
    Tags
    APEX2 and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167288-plasmid-map-sequence-id-330940

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  35. MCS-3_Endofin_LgBiT

    Plasmid
    #182574
    Purpose
    This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.
    Depositor
    Bradley McConnell
    Article
    Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1
    Insert
    zinc finger FYVE-type (ZFYVE16 Human)
    Use
    Tags
    LgBiT (Large BiT)
    Expression
    Mammalian
    Mutation
    FYVE domain Q739-K806
    Promoter
    Herpes simplex virus (HSV)
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    182574-plasmid-map-sequence-id-450120

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  40. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

    Plasmid
    #172506
    Purpose
    Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    DisCas7-11 bacterial expression vector with golden gate site for guides
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172506-plasmid-map-sequence-id-344928

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  41. pAAV.GfaABC1D.PI.Lck-GFP.SV40 (AAV5)

    Viral Prep
    #105598-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GfaABC1D.PI.Lck-GFP.SV40 (#105598). In addition to the viral particles, you will also receive purified pAAV.GfaABC1D.PI.Lck-GFP.SV40 plasmid DNA. GfaABC1D-driven GFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Article
    Shigetomi et al J Gen Physiol. 2013 Apr 15.
    Promoter
    GfaABC1D
    Tags
    GFP
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 3251 - 3300 of 163546 results