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179,463 results

Showing: 3501 - 3550 of 179463 results

  2. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Publication
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    122650-plasmid-map-sequence-id-437776

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  3. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (AAV1)

    Viral prep
    #104493-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (#104493). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7b-WPRE plasmid DNA. Synapsin-driven, Cre-dependent jGCaMP7b expression. jGCaMP7b exhibits the brightest resting fluorescence and can be used for imaging of small neuronal processes (dendrites and axons). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  5. pLenti6.2_miRFP670

    Plasmid
    #113726
    Purpose
    Expresses the fluorescent protein miRFP670 in a lentiviral backbone
    Depositor
    Vanessa LaPointe
    Insert
    miRFP670
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    113726-plasmid-map-sequence-id-218247

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  7. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV Retrograde)

    Viral prep
    #104492-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pICE-HA-MRE11-WT

    Plasmid
    #82033
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    82033-plasmid-map-sequence-id-490398

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  33. pAAV-hSyn-GRAB-gDA3m (AAV1)

    Viral prep
    #208698-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB-gDA3m (#208698). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB-gDA3m plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Publication
    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Promoter
    Syn
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  38. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    140002-plasmid-map-sequence-id-275516

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  43. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid
    #128604
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.
    Depositor
    Marvin Tanenbaum
    Publication
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    Expression
    Mammalian
    Promoter
    Pcmv
    Available since
    Aug. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    128604-plasmid-map-sequence-id-457471

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Showing: 3501 - 3550 of 179463 results