Skip to main content
Addgene
Showing: 4151 - 4200 of 19992 results

  3. HTR2A-Tango

    Plasmid
    #66409
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR2A (HTR2A Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66409-plasmid-map-sequence-id-244394

    Enlarge

  12. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

    Enlarge

  13. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)

    Viral Prep
    #92379-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .
    Depositor
    David Root, John Doench
    Article
    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:
    Promoter
    Tags
    None
    Available since
    Aug. 3, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. hprtSAGFP

    Plasmid
    #41594
    Purpose
    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences
    Depositor
    Maria Jasin
    Article
    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.
    Insert
    SA-GFP reporter
    Use
    Mouse Targeting; Dsb repair reporter substrate
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    41594-plasmid-map-sequence-id-228244

    Enlarge

  20. lentiGuide-Hygro-dTomato

    Plasmid
    #99376
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99376-plasmid-map-sequence-id-191027

    Enlarge

  24. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV5)

    Viral Prep
    #105545-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    HI
    Tags
    EGFP
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-FLEX-ArchT-tdTomato (AAV5)

    Viral Prep
    #28305-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-FLEX-ArchT-tdTomato (#28305). In addition to the viral particles, you will also receive purified pAAV-FLEX-ArchT-tdTomato plasmid DNA. CAG-driven Cre-dependent ArchT-tdTomato expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Han et al Front Syst Neurosci. 2011;5:18. Epub 201
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  32. pT18mobsacB

    Plasmid
    #72648
    Purpose
    sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72648-plasmid-map-sequence-id-399780

    Enlarge

  33. Halo-TOMM20-N-10

    Plasmid
    #123284
    Purpose
    Localization: Outer Mitochondrial Membrane
    Depositor
    Kevin McGowan
    Insert
    TOMM20 (TOMM20 Human)
    Use
    Tags
    HaloTag7
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    123284-plasmid-map-sequence-id-242688

    Enlarge

  34. pcDNA-3-VEGFR3

    Plasmid
    #119230
    Purpose
    Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.
    Depositor
    Kari Alitalo
    Insert
    VEGFR3 (FLT4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119230-plasmid-map-sequence-id-231667

    Enlarge

  37. pMH-Halo tag

    Plasmid
    #154144
    Purpose
    Gateway compatible mammalian expression plasmid for halo tag fusion proteins
    Depositor
    Michael Huen
    Type
    Empty backbone
    Use
    Tags
    Halo tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154144-plasmid-map-sequence-id-309445

    Enlarge

  39. ADA

    Plasmid
    #39020
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADA (ADA Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  41. psfTn5

    Plasmid
    #79107
    Purpose
    bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations
    Depositor
    Sten Linnarsson
    Insert
    transposase 5
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    E54K and L372P
    Promoter
    Available since
    March 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    79107-plasmid-map-sequence-id-418885

    Enlarge

  42. pLJM1 EGFP-Tau

    Plasmid
    #108868
    Purpose
    Expresses EGFP linked to human Tau
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    human four-repeat tau lacking the N-terminal sequ…
    Promoter
    CMV
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108868-plasmid-map-sequence-id-237064

    Enlarge

Showing: 4151 - 4200 of 19992 results