165,872 results

1. pGL0_5 [pAL5000 ORI]

   Plasmid

   #198922

   Purpose

   Level 0 part

   Depositor

   Cultivarium Tools, Charlie Gilbert, Nili Ostrov

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pAL5000 ORI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-mDlx-ChR2-mCherry-Fishell-3

   Plasmid

   #83898

   Purpose

   ChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   ChR2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mDlx

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBabe neo TGFBR3-HA

   Plasmid

   #83095

   Purpose

   Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   TGFBR3 (TGFBR3 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

4. FH1-MGT#1-mVenus

   Plasmid

   #164094

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

   Insert

   MGT#1-mVenus
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiGuide Cherry

   Plasmid

   #170510

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Pavel Tolar

   Article

   Newman et al biorXiv 2021.03.02.433398

   Inserts

   S. pyogenes sgRNA cassette
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL4.23-GW

   Plasmid

   #60323

   Purpose

   This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHCD1B

   Plasmid

   #102953

   Purpose

   mammalian expression of CD1B

   Depositor

   Gennaro De Libero, Lucia Mori

   Article

   Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

   Insert

   CD1B cDNA (CD1B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

   Plasmid

   #60910

   Purpose

   dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   dSV40 Promoter

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJExpress414_GmPOPB

   Plasmid

   #92234

   Purpose

   Express wild type prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGW-TurboID-GOI

    Plasmid

    #209380

    Purpose

    Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV0328

    Plasmid

    #133489

    Purpose

    Expression of nucleo-cytoplasmic mCherry

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMPRA1-CMV-Clover

    Plasmid

    #53538

    Purpose

    Fluorescent reporter control for pMPRA1

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SauCas9 EMX1-pegRNA

    Plasmid

    #169857

    Purpose

    SauCas9 pegRNA for EMX1

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SauCas9 EMX1-pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-AcGFP-Sec61-IRES-Blast

    Plasmid

    #154009

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    acGFP-Sec61 (SEC61B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Reb_79 aka PAS620

    Plasmid

    #71225

    Purpose

    Reb_1 with RebA(Q91R)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA( G53A, Q91R)

    Promoter

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pWPT-nlsLacZ

    Plasmid

    #12261

    Depositor

    Didier Trono

    Insert

    LacZ
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJMD14

    Plasmid

    #138654

    Purpose

    phiOZJ integrating Streptomyces expression vector, intact RDF

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

    Plasmid

    #70085

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

    Promoter

    SV40

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQCi-mB2m-sgRNA

    Plasmid

    #154090

    Purpose

    pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA targeting murine B2M locus
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. NM R2E2 GFP

    Plasmid

    #1208

    Depositor

    Susan Lindquist

    Article

    Liu et al Nature 1999 Aug 5;400(6744):573-6.

    Insert

    sup35 NM domain (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    2 additional copies of second oligopeptide repeat…

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcdna DSG-2 Tailless

    Plasmid

    #89466

    Purpose

    DSG-2 tailless control

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHR- flag-Lag16-iCAM-1-Tether

    Plasmid

    #205196

    Purpose

    This vector encodes of the synCAM Tether (iCAM1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 Tether and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-gp32-H6

    Plasmid

    #163912

    Purpose

    Expresses T4 gp32 for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    gp32
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. coel::RFP

    Plasmid

    #8938

    Depositor

    Piali Sengupta

    Article

    Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

    Insert

    unc-122 promoter (unc-122 Nematode)
    

    Use

    Tags

    RFP

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Nov. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCI-neo-HA-hUVRAG

    Plasmid

    #24290

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    UV irradiation resistance-associated gene (UVRAG Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pHAGE-CMV-Flag-TLK2_IDG-K

    Plasmid

    #135278

    Purpose

    Gateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-TLK2 (TLK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p2T-CMV-miniSdd6-BE4max-BlastR

    Plasmid

    #204850

    Purpose

    For cytosine base editing using miniSdd6 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EF06R

    Plasmid

    #42940

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    Human L1 element (L1RE1 Human)
    

    Use

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV MIE, L1 5'UTR

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pICSL11024 (pICH47732::NOSp-NPTII-OCST)

    Plasmid

    #51144

    Purpose

    Confers KAN resistance in planta

    Depositor

    Jonathan D Jones

    Insert

    NOSp-NPTII-OCST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mRNA-CleanCap entry vector

    Plasmid

    #188249

    Purpose

    Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMPRA2

    Plasmid

    #49350

    Purpose

    Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLL5.0 shCAV1-NLS-mCherry

    Plasmid

    #187257

    Purpose

    Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Caveolin-1 (Cav1 Mouse)
    

    Use

    Lentiviral

    Tags

    NLS-mCherry

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FH1-MGT#2-mCherry

    Plasmid

    #164097

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#2-mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

    Plasmid

    #115516

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAV.CamKII.GCaMP6s.WPRE.SV40

    Plasmid

    #107790

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcdna DSG-2 TS

    Plasmid

    #89465

    Purpose

    DSG-2 tension sensor, using TSmod inserted in human DSG-2

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. [UBC][NRE Pum][eGFP]

    Plasmid

    #97192

    Purpose

    The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    wild type Pumilio homology domain
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC (ubiquitin C)

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pQP-CiRISB

    Plasmid

    #138978

    Purpose

    Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMPRA2-CMV-Clover

    Plasmid

    #53539

    Purpose

    Fluorescent reporter control for pMPRA2

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGFP-coilin

    Plasmid

    #36906

    Depositor

    Greg Matera

    Article

    Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

    Insert

    coilin (COIL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV6-XL4 FLAG-CBLN4-Fc

    Plasmid

    #115769

    Purpose

    Expression of the extracellular domain of CBLN4 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    CBLN4 (CBLN4 Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. plRL58

    Plasmid

    #166886

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pICH41766

    Plasmid

    #48018

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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46. pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

    Plasmid

    #128174

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. Z-lock cofilin

    Plasmid

    #141137

    Purpose

    controlling the actin-severing ability of cofilin with light

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mCherry, Zdk2(132F), Cofilin S3A, LOV WT

    Promoter

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pMPRA3-CMV-Clover

    Plasmid

    #53540

    Purpose

    Fluorescent reporter control for pMPRA3

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    Clover GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. RAB14 shRNA

    Plasmid

    #61496

    Purpose

    to knockdown endogenous RAB14 expression levels

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 (RAB14 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEGFP-GFP11-Clathrin light chain

    Plasmid

    #70217

    Purpose

    Expresses GFP11-clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP11-Clathrin light chain (CLTA Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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