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161,511 results

Showing: 4501 - 4550 of 161511 results

  1. pYC1640

    Plasmid
    #158719
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158719-plasmid-map-sequence-id-320840

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  2. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)

    Viral Prep
    #104491-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Tags
    None
    Available since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV mGAD65(delE1) GFP WPRE SV40pA

    Plasmid
    #177316
    Purpose
    To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.
    Depositor
    Hirokazu Hirai
    Article
    Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10
    Insert
    mGAD65(delE1) promoter, GABAergic interneurons specific promoter
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    177316-plasmid-map-sequence-id-377835

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  7. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pTRE3G-bidirectional
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

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  8. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Promoter
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  10. mEGFP-Lifeact-7

    Plasmid
    #54610
    Purpose
    Localization: Actin, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mEGFP-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54610-plasmid-map-sequence-id-234554

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  12. pAdTrack-CMV

    Plasmid
    #16405
    Purpose
    Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.
    Depositor
    Bert Vogelstein
    Article
    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2008
    Availability
    Academic Institutions and Nonprofits only
    16405-plasmid-map-sequence-id-190476

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  13. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110403-plasmid-map-sequence-id-213570

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  18. CMV mRuby3-Synapsin1a

    Plasmid
    #187896
    Purpose
    For labeling pre-synaptic terminals
    Depositor
    Timothy Ryan
    Insert
    Synapsin1a
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187896-plasmid-map-sequence-id-394752

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  21. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

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  22. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

    Plasmid
    #203348
    Purpose
    Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Molly Stevens, Christopher Grigsby
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203348-plasmid-map-sequence-id-399143

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  26. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid
    #225684
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.
    Depositor
    Ryan Mehl
    Inserts
    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    Use
    Methanomethylophilus alvus
    Tags
    Expression
    Mutation
    CTG125CTT, N166A, V168S, A223C, W239R
    Promoter
    GlnS and Lpp
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    225684-plasmid-map-sequence-id-450701

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  30. pAAV-Ef1a-Con/Fon-mCherry (AAV8)

    Viral Prep
    #137132-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-mCherry (#137132). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-mCherry plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    mCherry (Cre and Flp-dependent)
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only

  31. pH6-MBP-TEV-BirA

    Plasmid
    #179694
    Purpose
    Expresses His6 tagged MBP-BirA fusion protein
    Depositor
    Mark Arbing
    Insert
    BirA
    Use
    Tags
    His6-MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179694-plasmid-map-sequence-id-352195

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  34. mEmerald-Rad21-N-18

    Plasmid
    #54248
    Purpose
    Localization: Centromere, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Rad21 (RAD21 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  45. CIBN-CAAX

    Plasmid
    #79574
    Purpose
    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    CIBN (CIB1 Mustard Weed)
    Use
    Tags
    C-terminal CAAX-box
    Expression
    Mammalian
    Mutation
    K93A, R94A, K106A, K107A (please see depositor co…
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79574-plasmid-map-sequence-id-314173

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Showing: 4501 - 4550 of 161511 results