Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

171,702 results

Showing: 4501 - 4550 of 171702 results

  5. pcDNA3-Flag-Hsp40-S171A

    Plasmid
    #84999
    Purpose
    Hsp40/DnaJB1 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp40 (DNAJB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Serine 171 mutated to Alanine
    Promoter
    CMV
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. AAV-EF1a-BbChT (AAV9)

    Viral Prep
    #45186-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-EF1a-BbChT (#45186). In addition to the viral particles, you will also receive purified AAV-EF1a-BbChT plasmid DNA. Brainbow construct encoding mCherry and mTFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Dawen Cai, Joshua Sanes
    Article
    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi
    Promoter
    EF1a
    Tags
    mCherry, mTFP, or none
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. mKeima-Red-N1

    Plasmid
    #54597
    Purpose
    Localization: N1 Cloning Vector, Excitation: 440, Emission: 620
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mKeima-Red
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    54597-plasmid-map-sequence-id-251812

    Enlarge

  11. RHPMP139 (pLifeAct mEOS3.1)

    Plasmid
    #88839
    Purpose
    Localization by Super-Resolution microscopy of lifeAct fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    LifeAct
    Tags
    mEOS3.1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88839-plasmid-map-sequence-id-177489

    Enlarge

  13. pET28a_SYK

    Plasmid
    #224240
    Purpose
    Expression of human SYK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    syk (SYK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224240-plasmid-map-sequence-id-459259

    Enlarge

  14. pET28a_LYN

    Plasmid
    #224238
    Purpose
    Expression of human LYN Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224238-plasmid-map-sequence-id-458419

    Enlarge

  20. CHRM3-DuET

    Plasmid
    #213209
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM3 (CHRM3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213209-plasmid-map-sequence-id-430771

    Enlarge

  26. sfGFP-FYN-N-10

    Plasmid
    #56294
    Purpose
    Localization: Membrane Associated Kinase, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    FYN (FYN Human)
    Tags
    sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

    Plasmid
    #208953
    Purpose
    A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208953-plasmid-map-sequence-id-415742

    Enlarge

  33. PC1575

    Plasmid
    #232088
    Purpose
    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.
    Depositor
    Pranam Chatterjee
    Article
    Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi
    Insert
    uAb with cloning Esp3I restriction site
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232088-plasmid-map-sequence-id-464073

    Enlarge

  36. iCas

    Plasmid
    #84232
    Purpose
    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)
    Depositor
    Meng How Tan
    Article
    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038
    Insert
    Cas9
    Tags
    2A-OFP co-expression and ERT2-ERT2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    84232-plasmid-map-sequence-id-323254

    Enlarge

  39. pAAV-flex-taCasp3-TEVp (AAV1)

    Viral Prep
    #45580-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nirao Shah, Jim Wells
    Article
    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10
    Promoter
    EF1a
    Available Since
    Dec. 3, 2020
    Availability
    Academic Institutions and Nonprofits only

  40. pAAV-EGFP.T2A.NCLX

    Plasmid
    #181873
    Purpose
    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Inserts
    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    Use
    AAV and Adenoviral
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    synthetic hybrid CAG promoter and synthetic hybri…
    Available Since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    181873-plasmid-map-sequence-id-355952

    Enlarge

  41. PAS_RvSAHS1

    Plasmid
    #215645
    Purpose
    Expresses SUMO-tagged RvSAHS1 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS1
    Tags
    SUMO
    Expression
    Bacterial
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215645-plasmid-map-sequence-id-426092

    Enlarge

Showing: 4501 - 4550 of 171702 results