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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Publication

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Expression

Bacterial

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEF6-Acly

Plasmid

#70765

Purpose

Expresses mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Publication

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Promoter

EF1alpha

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Murine E-cadherin mCherry

Plasmid

#71366

Purpose

mammalian expression of murine E-caderin with mCherry fusion

Depositor

Alpha Yap

Publication

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

E-cadherin (Cdh1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

Plasmid

#69901

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Publication

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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PX404 Campylobacter jejuni Cas9

Plasmid

#68338

Purpose

Campylobacter jejuni Cas9

Depositor

Feng Zhang

Insert

CjCas9

Use

CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMV

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pUC18T-mini-Tn7T-Gm-dsRedExpress

Plasmid

#65032

Purpose

GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

dsRedExpress

Expression

Bacterial

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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phage ubc nls ha pcp gfp

Plasmid

#64539

Purpose

Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

Depositor

Jeffrey Chao

Publication

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

PCP

Use

Lentiviral

Tags

EGFP, FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Publication

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Expression

Mammalian

Promoter

CAG

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Publication

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has service

AAV1, AAV5, and AAV Retrograde

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pSUMO1

Plasmid

#52258

Purpose

Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 )
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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EF05J

Plasmid

#42941

Depositor

Eline Luning Prak

Publication

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

Negative control Functionally Dead Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

ORF1 R261A, R262A

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPR::rpsL

Plasmid

#44505

Purpose

A crRNA expression plasmid specific to the rpsL allele.

Depositor

Luciano Marraffini

Publication

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR::rpsL (rpsL )

Use

CRISPR; E.coli

Available since

April 16, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST/hCx43-EGFP-N1

Plasmid

#40907

Depositor

Robin Shaw

Publication

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 (GJA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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phage-cmv-cfp-24xpp7

Plasmid

#40652

Depositor

Robert Singer

Publication

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

CFP-24xPP7

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMSCVhygro-Cre

Plasmid

#34565

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Cre

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

March 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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EGFP-Tpr

Plasmid

#35024

Depositor

Larry Gerace

Publication

Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

Insert

Tpr (TPR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

Plasmid

#32149

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

FRT> STOP STOP FRT>

Expression

Insect

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMV-PTEN

Plasmid

#28298

Depositor

Maria-Magdalena Georgescu

Publication

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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SNX9-pmCherryC1

Plasmid

#27678

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

SNX9 (Snx9 Mouse)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR223-MET

Plasmid

#23889

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MET (MET Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Flag-HA-USP16

Plasmid

#22595

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP16 (USP16 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pROSA26-5'

Plasmid

#21715

Depositor

Philippe Soriano

Publication

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

ROSA26-5' insert (Gt(ROSA)26Sor Mouse)

Use

For use with mouse targeting vector prosa26-1

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 CDK7 HA (P#633)

Plasmid

#14647

Depositor

Matija Peterlin

Publication

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Insert

Cdk7 (CDK7 Human)

Tags

HA

Expression

Mammalian

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA-TRBP

Plasmid

#15666

Depositor

Dong-Yan Jin

Publication

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

TRBP (TARBP2 Human)

Expression

Mammalian

Available since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pJC6-GL3

Plasmid

#11979

Purpose

Reporter gene with mouse c-jun promoter.

Depositor

Ron Prywes

Publication

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
miR-146a

Plasmid

#15092

Depositor

David Baltimore

Publication

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

pre-miR-146a

Expression

Mammalian

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pGEX-4T1 SHP2 WT

Plasmid

#8322

Depositor

Ben Neel

Publication

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pSV Sport PPAR gamma 2

Plasmid

#8862

Depositor

Bruce Spiegelman

Publication

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

PPAR gamma (Pparg Mouse)

Expression

Mammalian

Available since

Feb. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pAAV-nEF-Con/Fon DREADD Gi-mCherry (AAV8)

Viral prep

#177672-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon DREADD Gi-mCherry (#177672). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon DREADD Gi-mCherry plasmid DNA. nEF-driven, Cre- and Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Promoter

nEF

Tags

mCherry

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only
pAAV-hIBA1a-GFP-miR124T

Plasmid

#214147

Purpose

AAV vector to restrict GFP expression in microglia.

Depositor

Chun-Li Zhang

Publication

Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

Has service

AAV5

Insert

GFP

Use

AAV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-mCherry (AAV1)

Viral prep

#114469-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Promoter

CaMKII

Tags

mCherry

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-ExRai-AKAR2

Plasmid

#161753

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

Depositor

Jin Zhang

Publication

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2

Expression

Mammalian

Promoter

CMV

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Publication

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Expression

Bacterial

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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BL21(DE3)-R3-pRARE2

Bacterial strain

#26242

Purpose

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Bacterial resistance

Chloramphenicol

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
Gamillus/ pcDNA3

Plasmid

#124837

Purpose

mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

Depositor

Takeharu Nagai

Publication

Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

Insert

Gamillus

Tags

No tag

Expression

Mammalian

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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FKBP-V5-AP-nes_pLX304

Plasmid

#120912

Purpose

Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

Depositor

Alice Ting

Publication

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

FKBP-V5-AP-NES

Use

Lentiviral

Expression

Mammalian

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX307 Luciferase

Plasmid

#117734

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Publication

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pHR CD19-FcGamma CAR

Plasmid

#113014

Purpose

for production of lentivirus encoding the CD19 CAR-Pfcgamma

Depositor

Ron Vale

Publication

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular GFP (CD19 Synthetic, Human, Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSBbi-pur H-2Kb

Plasmid

#111623

Purpose

Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

Depositor

Jon Yewdell

Insert

H2Kb

Expression

Mammalian

Promoter

EF1alpha

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pAAV.Syn.GCaMP6m.WPRE.SV40 (AAV9)

Viral prep

#100841-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Publication

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Promoter

Syn

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only
pAW13.lentiguide.mCherry

Plasmid

#104375

Purpose

mCherry vector for cloning in guides

Depositor

Richard Young

Publication

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

Depositor

David Sabatini

Publication

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pCDH-puro-EWS-FLI1

Plasmid

#102813

Purpose

Overexpress EWS-FLI1. 3rd generation lentiviral vector.

Depositor

Jialiang Wang

Publication

Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

Insert

EWS-FLI1 (EWSR1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

Enlarge
STARR-seq luciferase validation vector_ORI_empty

Plasmid

#99297

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

Enlarge
pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

Enlarge
pK170.AAV-TRE-Cre-WPRE (Supernova)

Plasmid

#85040

Purpose

For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc. Inducible with tetracycline or doxycycline.

Depositor

Takuji Iwasato

Publication

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nlsCre-WPRE

Use

AAV

Expression

Mammalian

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
EGFP-E-Syt2

Plasmid

#66831

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Publication

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt2 (ESYT2 Human)

Tags

GFP

Expression

Mammalian

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
iRFP-PH-PLCdelta1

Plasmid

#66841

Purpose

expression of near-infrared PI(4,5)P2 biosensor

Depositor

Pietro De Camilli

Publication

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

PH-PLCdelta1

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge

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