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pENTR223-BRRF2

Plasmid

#195820

Purpose

Gateway Entry Clone of Epstein-Barr virus BRRF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BRRF2 (BRRF2 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BXLF1

Plasmid

#195825

Purpose

Gateway Entry Clone of Epstein-Barr virus BXLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BXLF1 (BXLF1 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsLSm1_J

Plasmid

#146676

Purpose

Mammalian Expression of HsLSm1

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsLSm1 (LSM1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMal-mRuby3

Plasmid

#209348

Purpose

Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagII

Depositor

Lucia Strader

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pJ02B2Gm_AE

Plasmid

#66063

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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Pado pcDNA3.1

Plasmid

#79663

Purpose

Proton Channel Fluorescent Voltage Indicator

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Pado Green

Expression

Mammalian

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pOTTC1534 pscAAV mU6 shRNA(PKCd) CMV-IE Nuc-EYFP

Plasmid

#135562

Purpose

An AAV vector expressing shRNA vs rat PKCd and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

shRNA (mouse PKCd)
Nuc-EYFP

Use

AAV and RNAi

Tags

3xNLS

Expression

Mammalian

Promoter

CMV-IE and mU6

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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C-His-GvpC-TEV

Plasmid

#153296

Purpose

Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

TEV-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) 21 bp TEV recognition and cleavage site flanke…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJ02B2Gm_EF

Plasmid

#66064

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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VPX-mRNA

Plasmid

#227679

Purpose

plasmid for VPX mRNA IVT

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

VPX (HIV2gp3 )

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4(K190R)

Plasmid

#58317

Purpose

FLAG-tagged mouse TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pEVP-9

Plasmid

#254821

Purpose

EV Packaging vector with five ?N repeats and human CLYBL scaffold

Depositor

Connie Cepko

Article

Ma et al Proc Natl Acad Sci U S A. 2026 Apr 14;12

Insert

CLYBL

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEVPC-5

Plasmid

#254820

Purpose

EV Packaging vector with I3-01 nanocage, NS3/4A wild-type protease, and λN

Depositor

Connie Cepko

Article

Ma et al Proc Natl Acad Sci U S A. 2026 Apr 14;12

Insert

I3-01

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCALSL-DsRed

Plasmid

#254812

Purpose

Cre-dependent DsRed reporter for titer of EV loading with Cre mRNA

Depositor

Connie Cepko

Article

Ma et al Proc Natl Acad Sci U S A. 2026 Apr 14;12

Insert

DsRed

Use

Cre/Lox

Expression

Mammalian

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pRBC-PINBodyC-Tau(297-441)-WT-his6

Plasmid

#253344

Purpose

Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 297-441

Depositor

Richard Cooley

Article

Vesely et al Protein Sci. 2026 Apr;35(4):e70520. doi:

Insert

Tau 2N4R (MAPT Human)

Tags

His6 and PINBodyC

Expression

Bacterial

Mutation

deleted amino acids 1-296

Promoter

T7

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-DEST-IRES-Hygro

Plasmid

#253775

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-HA-5'-PRPF8

Plasmid

#244426

Purpose

5' AAVLINK plasmid for PRPF8 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

PRPF8 (PRPF8 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pCAT.703

Plasmid

#240217

Purpose

Level T integrative acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.644

Plasmid

#240218

Purpose

Level 1 Down Flank acceptor

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.620

Plasmid

#240219

Purpose

Level 1 Up Flank acceptor

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)-FLEx(loxP)-mCherry(nuclear)-2A-N2cG

Plasmid

#248663

Purpose

Helper virus for monosynaptic tracing; Expresses nuclear mCherry and Rabies Glycoprotein (G) of CVS-N2c strain in Cre-expressing cells under the CAG promoter.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Inserts

N2c(G)
nuc-mCherry

Use

AAV

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCA1.552

Plasmid

#220334

Purpose

pOP2-Prom- Level 1 Position 2 operon acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0.340

Plasmid

#200628

Purpose

Level 0 part - RBS

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Insert

RBS*

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pC0.341

Plasmid

#200629

Purpose

Level 0 part - RBS

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Insert

RBS_B0034

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCA1.264

Plasmid

#200610

Purpose

pOP4 - Level 1 Position 4 operon acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.265

Plasmid

#200611

Purpose

pOP5 - Level 1 Position 5 operon acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.266

Plasmid

#200612

Purpose

pOP6 - Level 1 Position 6 operon acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.268

Plasmid

#200614

Purpose

pTL1 - End Linker 1 Terminator acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.269

Plasmid

#200615

Purpose

pTL2 - End Linker 2 Terminator acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.270

Plasmid

#200616

Purpose

pTL3 - End Linker 3 Terminator acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.271

Plasmid

#200617

Purpose

pTL4 - End Linker 4 Terminator acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.272

Plasmid

#200618

Purpose

pTL5 - End Linker 5 Terminator acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.273

Plasmid

#200619

Purpose

pTL6 - End Linker 6 Terminator acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA0.324

Plasmid

#200621

Purpose

Level 0 TSS promoter acceptor vector is LacZ compatible blue/white screening, overhangs allow for assembly with RBS parts in acceptor vector pCA0.325,

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA0.325

Plasmid

#200622

Purpose

Level 0 RBS acceptor vector with LacZ compatible blue/white screening.

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.261

Plasmid

#200607

Purpose

pOP1 - Level 1 Position 1 operon acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.262

Plasmid

#200608

Purpose

pOP2 -Level 1 Position 2 operon acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA1.263

Plasmid

#200609

Purpose

pOP3 - Level 1 Position 3 operon acceptor vector

Depositor

Alistair McCormick

Article

Astbury et al bioRxiv 2026.02.24.707249

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_BetaActin_ALFA_229230

Plasmid

#250439

Purpose

Expresses tagged beta cytoplasmic actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T229/A230.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Beta Cytoplasmic Actin (ACTB Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pDisplay-hpGRISZ

Plasmid

#248027

Purpose

Plasmid for mammalian expression of hpGRISZ (synaptic zinc sensor)

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2026 Mar 3. doi: 10.1021/acsse

Insert

hpGRISZ

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLminP_Luc2P_RE58

Plasmid

#247035

Purpose

IRF1 pathway reporter; lentiviral expression of IRF1 DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IRF1

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonr201_PARP1

Plasmid

#240317

Purpose

Entry vector for Gateway with PARP1

Depositor

Junjie Chen

Article

Nie et al Elife. 2024 Apr 5;12:RP89303. doi: 10.75

Insert

PARP1 (PARP1 Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBL071

Plasmid

#239089

Purpose

Expresses cCadC-AB42 under the control of Ptet

Depositor

Tina Wang

Article

Lee et al Nat Chem Biol. 2025 Jul 15. doi: 10.1038

Insert

cCadC-AB42

Use

Synthetic Biology

Tags

cCadC fused to AB42

Promoter

PTet

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SM469_EF1A-dHyperLbCas12a-T2A-BlastR

Plasmid

#244221

Purpose

Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VEE-mScarlet3-IRES-PuroR

Plasmid

#242415

Purpose

Spectral unmixing: mScarlet3 only

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-PuroR

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-H2B-mStayGold-WPRE (JDW 1384)

Plasmid

#242550

Purpose

Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mStayGold (monomeric StayGold)

Use

Gateway Cloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG-mLRRC45 (158 a.a.)

Plasmid

#240067

Purpose

ITT reporter plasmid for cold and osmotic stress

Depositor

Hodaka Fujii

Article

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

LRRC45 C-terminal 158 a.a. (Lrrc45 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Hygro-TRE>hHES3:T2A:EGFP

Plasmid

#236630

Purpose

Hygromycin selected lentiviral construct with tetracyclin-inducible human HES3

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

HES3 (HES3 Human)
EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized based on a variant of wildtype GF…

Promoter

TRE

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5_mutCKAR3

Plasmid

#238412

Purpose

Mammalian expression of a phosphorylation deficient (T to A) mutant of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-10

Plasmid

#228969

Purpose

For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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