29,744 results

1. pET29b-clbD

   Plasmid

   #114146

   Purpose

   Expresses ClbD-cHis

   Depositor

   Emily Balskus

   Article

   Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

   Insert

   ClbD
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET29b-clbG

   Plasmid

   #114150

   Purpose

   Expresses ClbG

   Depositor

   Emily Balskus

   Article

   Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

   Insert

   clbG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMRE-Tn7-167

   Plasmid

   #118579

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mClover3
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGBW-m4046852

   Plasmid

   #145584

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. p404ADH1

   Plasmid

   #15970

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   ADH1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCRISPRed pRSETB

   Plasmid

   #159454

   Purpose

   Expresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRed

   Depositor

   Oliver Griesbeck

   Article

   Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

   Insert

   mCRISPRed
   

   Use

   Tags

   10 x His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET29b-clbBpks

   Plasmid

   #114154

   Purpose

   Expresses the PKS module of ClbB

   Depositor

   Emily Balskus

   Article

   Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

   Insert

   clbB
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Only contains the PKS module of ClbB

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQTEV-PDAP1

   Plasmid

   #31586

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   PDGFA associated protein 1 (PDAP1 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSARSF53-110

   Plasmid

   #61813

   Purpose

   TvoVMAN11 intein

   Depositor

   Hideo Iwai

   Article

   Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

   Insert

   TvoVMAN11 intein
   

   Use

   Tags

   H6-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSABAD14-98

    Plasmid

    #61827

    Purpose

    TvoVMAdN11 intein

    Depositor

    Hideo Iwai

    Article

    Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

    Insert

    TvoVMAdN11 intein
    

    Use

    Tags

    GB1-H6

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pL0M-S-mNeonGreen-EC18153

    Plasmid

    #137075

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. H6-auxilin

    Plasmid

    #62571

    Purpose

    6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteria

    Depositor

    Ernst Ungewickell

    Article

    Schroder et al Eur J Biochem. 1995 Mar 1;228(2):297-304

    Insert

    Auxilin
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    *see comment below

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pTRC-LIC

    Plasmid

    #62343

    Purpose

    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter from pKK233-2

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSC27

    Plasmid

    #117478

    Purpose

    mobilizable, SRB-stable, kanamycin-resistant cloning vector

    Depositor

    Judy Wall

    Article

    Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_DNASE1_Exo-endo-phos

    Plasmid

    #110004

    Purpose

    Protein expression and purification of DNASE1_Exo-endo-phos

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    DNASE1_Exo-endo-phos (DNASE1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAcCAST-sgRNA_entry (CJT83)

    Plasmid

    #181785

    Purpose

    Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pgRNA-R-gfp

    Plasmid

    #220927

    Purpose

    Expresses gfp using J23119 promoter and pgRNA_AT backbone

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    superfolder GFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTHSSe_53

    Plasmid

    #109251

    Purpose

    pUC BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Mut2-RS

    Plasmid

    #182882

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

    Insert

    Mut2-RS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MP5

    Plasmid

    #69677

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA ugi CDA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTCF200 Mouse beta-2 microglobulin

    Plasmid

    #180462

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Mouse beta-2 microglobulin
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOGG005

    Plasmid

    #113980

    Purpose

    Level 1 high copy number vector for E. coli expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBJ6

    Plasmid

    #167135

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BB3nK_ext_AD

    Plasmid

    #108687

    Purpose

    Plasmid for assembly of donor DNA templates consisting of three expression units

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti_EGFP-P2A-HAUS6_IRES_Blast

    Plasmid

    #182886

    Purpose

    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (WT full length)

    Depositor

    Daniel Gerlich

    Article

    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

    Insert

    HAUS 6 (HAUS6 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha core

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMCSGC-GB1

    Plasmid

    #186786

    Purpose

    E. coli expression vector containing N-terminal 6His-GB1-TEV fusion.

    Depositor

    Alexei Savchenko

    Article

    Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAC1749

    Plasmid

    #127983

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSFDuetG-HsNot1iso2(MCSI)_O

    Plasmid

    #147088

    Purpose

    Bacterial Expression of HsNot1iso2

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso2 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p2CT-His-MBP-Hhe_Cas13a_WT

    Plasmid

    #91871

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    HheCas13a (HheC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GST-PS-Grb2 1-154 (XSB382)

    Plasmid

    #78510

    Purpose

    Construct for expressing Grb2 lacking the C-terminal SH3 domain in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Grb2 1-154 (GRB2 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAJM.715

    Plasmid

    #108516

    Purpose

    PTac-YFP Reporter. IPTG sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKD227

    Plasmid

    #90954

    Purpose

    Expresses S. baltica TtrS under the Ptac promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Insert

    Tetrathionate sensor kinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSL4369 (pEffector_TnpB2,ωRNA)

    Plasmid

    #210871

    Purpose

    Expresses GstTnpB2 and native ωRNA from constitutive promoters

    Depositor

    Samuel H. Sternberg

    Article

    Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

    Insert

    GstTnpB2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEX4T1-Smurf2 wt

    Plasmid

    #13504

    Depositor

    Jeff Wrana

    Article

    Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

    Insert

    SMURF2 (SMURF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pPbcsx28

    Plasmid

    #89909

    Purpose

    Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx28
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBP-ORF-xylE

    Plasmid

    #112780

    Purpose

    Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    xylE open reading frame
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMApra-Ptac

    Plasmid

    #218357

    Purpose

    Empty vector with lacIq-Ptac expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBiex-1 Myosin VI HMM_eGFP

    Plasmid

    #82714

    Purpose

    Expresses Myosin VI HMM with a C-terminal eGFP.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    Myosin VI
    

    Use

    Tags

    FLAG purification tag and eGFP

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFG036 + pFG051

    Plasmid

    #137997

    Purpose

    Transposon mutagenesis plasmid and the cI repressor expression plasmid

    Depositor

    Sébastien Rodrigue

    Article

    Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

    Insert

    Tn5 tnp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GST PTBP1 isoform 4

    Plasmid

    #108594

    Purpose

    Recombinant GST tagged protein production

    Depositor

    Chris Smith

    Article

    Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

    Insert

    PTBP1 isoform 4 (PTBP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    isoform 4 (isoform a)

    Promoter

    tac

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET26b-Granzyme A (S-A)

    Plasmid

    #8825

    Depositor

    Judy Lieberman

    Article

    Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

    Insert

    Human Granzyme A inactive (GZMA Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    changed Serine 184 to Alanine

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

44. pB3.P11

    Plasmid

    #69577

    Purpose

    OT-I TCR beta chain cDNA in pCRScript

    Depositor

    Francis R. Carbone

    Article

    Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

    Insert

    TCR beta cDNA clone B3 (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCasX1

    Plasmid

    #87685

    Purpose

    p15A plasmid expressing CasX locus from Deltaproteobacteria

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasX1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDEST17-UbE2H

    Plasmid

    #15752

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2H (UBE2H Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pTrc99S-ssDsbA-CRM197-4xDQNAT

    Plasmid

    #128403

    Purpose

    Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pTSA-C

    Plasmid

    #17329

    Depositor

    Takeshi Sano

    Article

    Reznik et al Bioconjug Chem. . 12(6):1000-4.

    Insert

    streptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated to include only amino acids 16-133 of m…

    Promoter

    Available since

    Feb. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

49. pRU1099

    Plasmid

    #14464

    Depositor

    Philip Poole

    Article

    Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

    Insert

    unstable gfp-mut3.1 AAV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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50. pJUMP44-2A(sfGFP)

    Plasmid

    #126988

    Purpose

    Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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