31,059 results
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Plasmid#172754PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-30
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZE31-DsRed-ms2x2
Plasmid#26248DepositorInsertms2 binding sites (x2)
ExpressionBacterialAvailable SinceOct. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pInh
Plasmid#199998PurposeInhibitor of transposase and integraseDepositorInsertLanding Pads
ExpressionBacterialAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-Uricase
Plasmid#182401PurposeBacterial expression of Vesicle Nucleating peptide-uricase fusionDepositorInsertVNp-uricase
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg1(NC)-SH2
Plasmid#46471DepositorInsertPLCg1(NC) (PLCG1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 542-759PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_Delta
Plasmid#175950PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsExpressionBacterialMutationDelta (Amino Acid 319-869)PromoterT7Available SinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCOLA banana sensor sfGFP
Plasmid#107367PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNADepositorInsertbanana-detecting toehold + sfGFP
ExpressionBacterialPromoterT7Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET tdTomato LIC cloning vector (u-tdTomato)
Plasmid#86926PurposepET LIC cloning vector for yORF-tdTomatoDepositorTypeEmpty backboneTagstdTomatoExpressionBacterialPromoterT7Available SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mPA-GFP-PA-Rac1
Plasmid#22022PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6X His and mPA-GFPExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPerimCh
Plasmid#227013PurposeExpresses periplasmic mCherryDepositorInsertPelB-mCherry, arabinose inducible cytosolic GFP
ExpressionBacterialPromoterrpsM and pBADAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSALECTNK-TEM1(S70A,D179G)/csTEM1
Plasmid#81163PurposepSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1DepositorInsertTEM-1 beta-lactamase
TagsCodon swapped TEM-1 from H26 to W290 and His6xExpressionBacterialMutationDeleted the sec transport tag. Plasmid encodes H2…Promoterlac promoterAvailable SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-EFTs
Plasmid#31173DepositorInsertE. coli elongation factor Ts
TagsHisExpressionBacterialAvailable SinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
EcMBP165-cpGFP.PPYF.T203V.pRSET
Plasmid#33373Purposea single-wavelength maltose sensor constructed from E. coli MalE and cpGFPDepositorInsertImproved MBP based Maltose Biosensor
Tags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted between AA1…PromoterT7Available SinceDec. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJBL7004
Plasmid#136943PurposeMalachite green aptamer, T7 promoterDepositorInsertmalachite green aptamer (MGA)
UseSynthetic BiologyExpressionBacterialPromoterpT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
H6-auxilin
Plasmid#62571Purpose6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteriaDepositorInsertAuxilin
Tags6XHisExpressionBacterialMutation*see comment belowAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PTPN22_Y-phosphatase
Plasmid#110048PurposeProtein expression and purification of PTPN22_Y-phosphataseDepositorInsertPTPN22_Y-phosphatase (PTPN22 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBNG2
Plasmid#149473PurposeBroad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertNahR(AM)-GFP
UseSynthetic BiologyExpressionBacterialPromoterPSalTTCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
TTC5
Plasmid#39064PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU+UIM, aa 287-481)
Plasmid#61406PurposeExpresses human OTUD1 (OTU+UIM domains) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-ttgR
Plasmid#62565PurposeFlavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAraTMwt
Plasmid#47514PurposeContains A2B L980A TMCY mutant, expresses wt AraC for heterodimer competition assayDepositorInsertIntegrin alpha2B L980A TM-CYTO
ExpressionBacterialPromoterTacAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
ETV2_pet28a
Plasmid#131665PurposeBacterial expression of ETS gene ETV2DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPclpBAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
TJF229
Plasmid#132575PurposepZE1-oxySp-mCherry-proD-oxyRDepositorInsertsoxySp-mCherry-TermT1
proD-oxyR-TermT1
ExpressionBacterialPromoteroxySp and proDAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG318
Plasmid#115595Purposesurface-displayed Ag2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKB083
Plasmid#181952PurposeIPTG-inducible expression of PhoQDepositorInsertPhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
ExpressionBacterialPromoterPhoQ-Ptac; lacI-PlacIqAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PTK2b
Plasmid#224237PurposeExpression of human PTK2b Kinase in E. coliDepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJWV25
Plasmid#61045PurposeTo construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaADepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pL0-BvDODAα1
Plasmid#162528PurposeBeta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertDODAα1
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BCAR1-1/1
Plasmid#91418PurposeProtein expression and purification of human SH3 domain construct BCAR1-1/1DepositorInsertBCAR1-1/1 (BCAR1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(136)
Plasmid#44380DepositorInsertUAS1B8-TEF(136) promoter
UseSynthetic BiologyExpressionBacterialMutationN/APromoterUAS1B8-TEF(136)Available SinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pTCF120 H-2D(b)
Plasmid#180454PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b)
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK-GW
Plasmid#126929PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-FIS
Plasmid#51564PurposeExpression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertFIS
TagsIntein-Chiting Binding DomaninExpressionBacterialAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
Plasmid#105000PurposeExpresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
Tags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianPromoterTetCMVAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT021a
Plasmid#107895PurposeW2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0DepositorInsertPTetO-SD8-BE2, PLac-sgRNA1
ExpressionBacterialAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SIM)1
Plasmid#126954PurposeBacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
TagseGFPExpressionBacterialPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLIPrbs
Plasmid#17860DepositorInsertFLIPrbs
TagsECFP and EYFPExpressionBacterialMutationD208GAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluA-NP
Plasmid#165092Purposeprotein expression in E. coliDepositorInsertInfluenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
ExpressionBacterialAvailable SinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pF709-pET28a-Hs-FL-ETFbeta-NHis wt
Plasmid#85110Purposeexpression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-LSSmKate2
Plasmid#31909DepositorInsertLSSmKate2
TagsHISExpressionBacterialMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…Available SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
SETD1A
Plasmid#32868PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
Tags6X His and sortase A sorting motifExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_SYK
Plasmid#224240PurposeExpression of human SYK Kinase in E. coliDepositorAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LYN
Plasmid#224238PurposeExpression of human LYN Kinase in E. coliDepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNHA5
Plasmid#223647PurposeTurboID protein purificationDepositorInsertTurboID
Tags6HisExpressionBacterialPromoterLacIAvailable SinceOct. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNHA6
Plasmid#223648PurposeBioID protein purificationDepositorInsertBioID
Tags6HisExpressionBacterialPromoterLacIAvailable SinceOct. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNHA7
Plasmid#223649PurposebirA protein purificationDepositorInsertBioID_G118R
Tags6HisExpressionBacterialMutationG118RPromoterLacIAvailable SinceOct. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits