29,765 results
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Plasmid#140395PurposeExpresses SmtB protein under the control of T7-lacO promoterDepositorInsertT7-lacO-SmtB-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN3945
Plasmid#74699PurposeCircuit plasmid: 0x41DepositorInsertCircuit 0x41
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6v15
Plasmid#176212PurposeBacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6v15
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
TOPO mTagBFP2
Plasmid#68413PurposeTOPO construct expressing mTagBFP2 for generation of GMAP-compatible geneDepositorInsertmTagBFP2
UseGmapTags6xHisExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorInsertHero11 (C19orf53 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTHMT_hnRNPF_FL
Plasmid#139114Purposeexpress FL hnRNPF with N-terminal maltose binding protein tagDepositorInserthnRNPF (HNRNPF Human)
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO AMPKa (11-281)
Plasmid#177851PurposeBacterial Expression of AMPKa1DepositorInsertAMPK a1 (PRKAA1 Human)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk3
Plasmid#81012PurposeExpression of Zdk1 anchored to mitochondriaDepositorInsertZdk3
UseTags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCis_2+7+8+5
Plasmid#60589Purposea 4-integrase plasmidDepositorInsertCis_2+7+8+5
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBbB14a-GFP
Plasmid#126117PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
LaM8-AK74
Plasmid#159594PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at AK74
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pTW211b5
Plasmid#136927PurposeDirected evolution of SpCas9DepositorInsertCas9(N)-NpuN
UseCRISPRTagsExpressionBacterialMutationsee manuscriptPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSG289
Plasmid#115608Purposesurface-displayed Nb2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCD061
Plasmid#113315PurposeW108 scRNA.b1DepositorInsertW108 scRNA.b1_MS2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pZ8-Prp
Plasmid#74065PurposepZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanRDepositorInsertPropionate inducible promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterprpAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQacR-Q2
Plasmid#74690PurposeQacR-Q2 gateDepositorInsertPTac-RBSQ2-QacR-PQacR-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046955
Plasmid#145616PurposeBacterial Expression plasmid for RNA-dependent RNA polymeraseDepositorInsertRNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRE-Tn5-130
Plasmid#118516PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092677
Plasmid#153883PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stopDepositorInsertfragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationwt;handful of SNPs as watermarksPromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbB11a-GFP
Plasmid#126114PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23150Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE60-Exp5
Plasmid#12553DepositorInsertEXPORTIN-5 (XPO5 Human)
UseTagsHISExpressionBacterialMutationNONEPromoterAvailable sinceOct. 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationMissing first 408bpPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen0.7
Plasmid#78176PurposeExpresses rsGreen0.7 in E. coli strains.DepositorInsertrsGreen0.7
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in LT)
Plasmid#70033PurposepUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding regionDepositorInsertTSPyV full genome, linearized in Large T-antigen
UseCloning vectorTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
ETS2_pet28a
Plasmid#131664PurposeBacterial expression of ETS gene ETS2DepositorInsertETS2 (ETS2 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAN1201
Plasmid#74695PurposeCircuit backbone plasmid (LacZa insert)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPath-pR-integrase
Plasmid#200000PurposeEntry vector for biosynthetic pathway. pR-phiC31 integraseDepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP1189
Plasmid#119257Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-Uac
Plasmid#126959PurposeUniversal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPA1L
Plasmid#177660PurposeRecombinant protein synthesis of human HSPA1LDepositorInsertHSPA1L (HSPA1L Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR
Plasmid#119828PurposeProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBG-NHERF2
Plasmid#28292DepositorInsertNHERF2 (NHERF2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
PPDK
Plasmid#64037PurposeExpression of Pyruvate phosphate dikinase (PPDK) in E. coliDepositorInsertpyruvate phosphate dikinase
UseTagsnoneExpressionBacterialMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-174-566
Plasmid#104197PurposeExpresses human AR-174-566 in pGEX-3x bacterial expression vector as fusion protein with GSTDepositorInserthuman androgen receptor aa 174-566 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046814
Plasmid#145601PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKCiR5
Plasmid#149462PurposeBroad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCinR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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His6-Twin-Strep-SUMO-pET28a-EfaCas2
Plasmid#112794PurposeTo express enterococcus faecalis Cas2 protein in E. coliDepositorInsertEfaCas2
UseTagsHis6-Twin-Strep-SUMOExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMD5
Plasmid#138652PurposephiBT1 integrating Streptomyces expression vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPermE* variant sequence from pDA1652Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB18cmBFP1A5
Plasmid#14035DepositorInsertBFP1A5
UseYeast/bacteria optimized codonsTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EC12WTC9A
Plasmid#47924PurposeExpresses E-cadherin EC12 WT with C9A mutation in E.Coli.DepositorInsertE-Cadherin EC12
UseTags6xHis and TEV CleavageExpressionBacterialMutationchanged Cysteine 9 to AlaninePromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1360
Plasmid#119278Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only