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31,059 results

Showing: 7901 - 7950 of 31059 results

  3. pRKO

    Plasmid
    #49456
    Purpose
    loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions
    Depositor
    Marc Gartenberg
    Article
    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1
    Insert
    loxP-kanMX-loxP cassette
    Use
    Cre/Lox
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49456-plasmid-map-sequence-id-73667

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  6. pOPINE-3C-YFP

    Plasmid
    #214028
    Purpose
    Expression of fluorescent fusion proteins
    Depositor
    Ray Owens
    Insert
    YFP
    Tags
    Hexahistidine
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214028-plasmid-map-sequence-id-422812

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  7. pAL-pck_NT

    Plasmid
    #74069
    Purpose
    pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pck (NT)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pIYN3

    Plasmid
    #197099
    Purpose
    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.
    Depositor
    Kensaku Sakamoto
    Article
    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;
    Insert
    TyrRS variant, Nap3 tRNA
    Expression
    Bacterial
    Mutation
    Ala70, Thr158, Ser159, Arg286 (TyrRS)
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    197099-plasmid-map-sequence-id-387837

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  17. pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

    Plasmid
    #168153
    Purpose
    Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB and TnsC)
    PmcTniQ
    Expression
    Bacterial
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168153-plasmid-map-sequence-id-330740

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  18. pColdII-SIRPT1-Bubalus bubalis

    Plasmid
    #75350
    Purpose
    Nt His-tag Bubalus bubalis Sacsin 86-1364
    Depositor
    Kalle Gehring
    Insert
    Sacsin Bubalus bubalis 86-1364
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75350-plasmid-map-sequence-id-142906

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  24. 5H9M (SIAH2)

    Plasmid
    #79521
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIAH2 (SIAH2 Human)
    Tags
    8xHis-Thrombin-SUMO-TEV
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. KLHL3 (KLHL3A-c003)

    Plasmid
    #110251
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL3 (KLHL3 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110251-plasmid-map-sequence-id-212338

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  30. pJJS005

    Plasmid
    #188328
    Purpose
    PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    mIAA7::mNeonGreen::Linker
    Expression
    Bacterial
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188328-plasmid-map-sequence-id-370751

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  32. cr3Ec

    Plasmid
    #184860
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, gam, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184860-plasmid-map-sequence-id-363182

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  42. pDule2-3-nitroTyrosine (5B)

    Plasmid
    #85499
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85499-plasmid-map-sequence-id-169856

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  44. pE5c

    Plasmid
    #17465
    Purpose
    modified pENTR vector for C-terminal ECFP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    ECFP
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17465-plasmid-map-sequence-id-8633

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Showing: 7901 - 7950 of 31059 results