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Bacterial Expression

29,771 results

Showing: 7901 - 7950 of 29771 results

  1. KDM4D

    Plasmid
    #73247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KDM4D (KDM4D Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam02375:JmjN 18-64, pfam02373:JmjC 182-295
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73247-plasmid-map-sequence-id-137202

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  2. HK3

    Plasmid
    #25303
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HK3:R480-R922 (HK3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids R480-R922
    Promoter
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pET19-MTdTc302

    Plasmid
    #207458
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    MTdTc302 (Dntt Mouse)
    Use
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    207458-plasmid-map-sequence-id-407772

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  6. pELlR1

    Plasmid
    #172569
    Purpose
    Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LacI-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLlacO-1
    Available since
    March 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    172569-plasmid-map-sequence-id-347325

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  7. pELlR2

    Plasmid
    #172555
    Purpose
    Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Kanamycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LacI-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLlacO-1
    Available since
    Nov. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    172555-plasmid-map-sequence-id-346737

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  8. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid
    #101211
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101211-plasmid-map-sequence-id-198053

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  9. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid
    #101204
    Purpose
    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101204-plasmid-map-sequence-id-198050

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  10. PTP1B-1-401

    Plasmid
    #78683
    Purpose
    bacterial expression of PTP1B aa 1-401
    Depositor
    Eli Chapman
    Insert
    PTPN1 (PTPN1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    delta 402-435
    Promoter
    araBAD
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78683-plasmid-map-sequence-id-152426

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  14. HDAC6 (3GV4)

    Plasmid
    #25297
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC6_42 (HDAC6 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 1109-1215
    Promoter
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pGGC_RT_mScarlet

    Plasmid
    #136967
    Purpose
    Goldengate/Greengate CDS module, containing mScarlet
    Depositor
    Klaus Theres
    Insert
    mScarlet
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136967-plasmid-map-sequence-id-269105

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  28. pHS1543

    Plasmid
    #205963
    Purpose
    NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    DinG HNH Cascade with His14-tagged Csf3
    Use
    Tags
    14xHis on Csf3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205963-plasmid-map-sequence-id-415370

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  32. pE4n

    Plasmid
    #17458
    Purpose
    modified pENTR vector for N-terminal GST tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    GST and Thrombin site
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17458-plasmid-map-sequence-id-8626

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  35. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid
    #128402
    Purpose
    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Use
    Tags
    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128402-plasmid-map-sequence-id-263357

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  41. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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  46. T5-sgRNA

    Plasmid
    #113655
    Purpose
    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.
    Depositor
    Jeffrey Barrick
    Article
    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531
    Insert
    promoter T5 and sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113655-plasmid-map-sequence-id-218814

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  47. SMS

    Plasmid
    #25234
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SMS_01
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  50. HUWE1

    Plasmid
    #25211
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    huwe1.4005.4374 (HUWE1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    HECT domain
    Promoter
    Available since
    Feb. 18, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7901 - 7950 of 29771 results