32,671 results
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Plasmid#124842PurposeExpresses Aspergillus terreus CAD in E.coliDepositorInsertcis-aconitate decarboxylase
TagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-11, synthetic codon optimiz…PromoterT7Available SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6T7G
Plasmid#71463PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and MammalianPromoterhuman U6/phage T7Available SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRAV16
Plasmid#123389Purposeconstruction of TCRDepositorAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAV13-1
Plasmid#123386Purposeconstruction of TCRDepositorAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SUMO_MT3
Plasmid#200303PurposeBacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2delBRCT
Plasmid#173939PurposeBacterial expression of PARP1 lacking Zn2 and BRCT domainsDepositorInsertPARP1delZn2delBRCT (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 97-213 and 374-520; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK556
Plasmid#74277PurposeProduces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)DepositorInsertformylglycinamidine ribonucleotide synthetase
TagsHis6ExpressionBacterialMutationchanges alanine-1246 to serine, numerous silent m…PromoterT7Available SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-TIAM1 DH/PH
Plasmid#81129Purposemammalian expression of Zdk1 tagged Tiam1 Dh/PHDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-gSTEP1
Plasmid#159376PurposeExpresses gSTEP1 in E. coliDepositorInsertgSTEP1
Tags6x His tagExpressionBacterialPromoterT7Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
Plasmid#105000PurposeExpresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
Tags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianPromoterTetCMVAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMI91
Plasmid#74888PurposeShuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more informationDepositorInsertTitin I27 x 9 (TTN Human)
TagsHis-tag and Strep-tag IIExpressionBacterialPromoterT7 promoterAvailable SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRT7 NT Topo arabinose
Plasmid#73446PurposeUsed for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptidesDepositorTypeEmpty backboneTagsGST (N-terminal), 6xHis (C-terminal)ExpressionBacterialPromoterpBadAvailable SinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP709
Plasmid#79986PurposeMonomeric near-infrared fluorescent protein miRFP709 in bacterial plasmidDepositorInsertmiRFP709
ExpressionBacterialPromoteraraBAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P**-R
Plasmid#189851PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP**-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterP** (modified T7 phage gene 10)Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-cat-P-R
Plasmid#189853PurposeCre-inducible activation of cat for chloramphenicol resistance.DepositorInsertP-loxP-TT-loxP-R-cat
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterP (T7 phage gene 10)Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLRD14
Plasmid#208266PurposeProduction of syn-pimara-7,15-diene in E. coli MEV20DepositorInsertstHMGR
osCPS4
osKSL4
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNtCp-rbcL-PHLS-GPPS-aadA-accD
Plasmid#52471PurposeInserts the beta-phellandrene synthase gene, the geranyl diphosphate synthase gene, and aadA resistance in the rbcL-accD intergenic region in Nicociana tabacumDepositorInsertrbcL-PHLS-GPPS-aadA-accD
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET26-Ufd1-His
Plasmid#21266DepositorAvailable SinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNAJB1_DNAJ
Plasmid#109918PurposeProtein expression and purification of DNAJB1_DNAJDepositorInsertDNAJB1_DNAJ
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET3a-His-DJ1
Plasmid#51488PurposeInducible expression of human His-DJ1 in E.coliDepositorAvailable SinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
GST-FOXO6
Plasmid#24381DepositorAvailable SinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS_gpN:SnoopTag
Plasmid#225195PurposeUsed for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid proteinDepositorInsertgpN homology arms and SnoopTag
ExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)
Plasmid#215759PurposeHDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)DepositorAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving
Plasmid#204810PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
ExpressionBacterialAvailable SinceDec. 18, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_GRB2_SH3
Plasmid#110015PurposeProtein expression and purification of GRB2_SH3DepositorInsertGRB2_SH3 (GRB2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (pGEX2T(TEV))
Plasmid#80630PurposeBacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197DepositorAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
ALC1-c005
Plasmid#173092PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBP-lldR
Plasmid#74095PurposeL-lactate repressor (LldR) from E. coli MG1655DepositorInsertlldR
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUltra-FPheKRS
Plasmid#136631PurposeGenetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteinsDepositorInsertFPheKRS
ExpressionBacterialPromoterTac1 promoterAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOGG043
Plasmid#133123PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.DepositorInsertPsNifH Synthetic consensus nifH promoter
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
HS-aPKC-CAAX-mKate2
Plasmid#105950Purposeheat shock inducible transgenesisDepositorInsertaPKC-CAAX
TagsmKate2ExpressionBacterialAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015
Plasmid#202023PurposeExpresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coliDepositorInsertAnabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
ExpressionBacterialMutationGvpA T6A-I48VAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET26_MraYaa
Plasmid#100166Purposerecombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target proteinDepositorInsertmraY (phospho-MurNAc-pentapeptide translocase)
TagsMBP-HisExpressionBacterialPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherryGGBsMTS
Plasmid#133625PurposeExpression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherryGGBsMTS
ExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN3945
Plasmid#74699PurposeCircuit plasmid: 0x41DepositorInsertCircuit 0x41
ExpressionBacterialAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7REwt
Plasmid#213935PurposeGST tagged AO7 encoding aa 126-258 for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
OA-1136J
Plasmid#153023PurposeExpress His-MBP-TEV-CasRx recombinant proteinDepositorInsertCasRx
TagsHis-MBP-TEVExpressionBacterialPromoterT7Available SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-TP
Plasmid#203589PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CTBP2
Plasmid#38799PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
EcMBP165-cpGFP.PPYF.pRSET
Plasmid#33372Purposea single-wavelength maltose sensor constructed from E. coli MalE and cpGFPDepositorInsertMBP based Maltose Biosensor
Tags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted between AA1…PromoterT7Available SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-T7-YhjH
Plasmid#79165PurposeExpresses YhjH under a T7 promoterDepositorInsertyhjH
ExpressionBacterialPromoterT7Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG28-J23151-AraC
Plasmid#102453PurposeExpresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.DepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pE5c
Plasmid#17465Purposemodified pENTR vector for C-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSA110
Plasmid#59784Purposedrives mCherry in C. elegans intestinal cellsDepositorInsertsExpressionBacterialAvailable SinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR2
Plasmid#112813Purposegateway cloning destination vector for expressing enhancer driven CD4-tdTomatoDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-hDDI2-D252N_pET11a
Plasmid#169122Purposeexpression of proteinDepositorAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421)
Plasmid#170643PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
ExpressionBacterialAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-11_enzymatic-domain
Plasmid#183382PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialPromoterT7Available SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only