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Showing: 11051 - 11100 of 29646 results

  4. NEDD4L-C2

    Plasmid
    #25615
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NEDD4L-C2 (NEDD4L Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains C2 domain
    Promoter
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pBad-CA TAG20

    Plasmid
    #105666
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 20
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105666-plasmid-map-sequence-id-205513

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  12. pRS415-KEX2

    Plasmid
    #10996
    Depositor
    Francisco Malagon
    Insert
    KEX2 (KEX2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Promoter
    Available since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
    10996-plasmid-map-sequence-id-6831

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  13. pLIC-SGC1

    Plasmid
    #39187
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39187-plasmid-map-sequence-id-160533

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  25. GPX1

    Plasmid
    #38864
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX1 (Gpx1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys49 -> Gly
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. NRDP1

    Plasmid
    #25620
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NRDP1 (RNF41 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains USP8 interaction Domain
    Promoter
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80060
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80060-plasmid-map-sequence-id-152594

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  36. pOPS0253

    Plasmid
    #115505
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115505-plasmid-map-sequence-id-224288

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  39. pCAH01SpR

    Plasmid
    #128159
    Purpose
    pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS
    Depositor
    Calvin Henard
    Article
    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    128159-plasmid-map-sequence-id-248974

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  41. pPP378

    Plasmid
    #158797
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-1 (D371A)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158797-plasmid-map-sequence-id-309517

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  46. 4L59

    Plasmid
    #60087
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3:JMC057-A08:C223215 (L3MBTL3 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. pGBW-m4046865

    Plasmid
    #153930
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153930-plasmid-map-sequence-id-307315

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  49. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Use
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Promoter
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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Showing: 11051 - 11100 of 29646 results