32,656 results
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Plasmid#112112PurposeExpresses hyperactive Tn5 transposase for next generation sequencing library preparationDepositorInsertTn5
TagsHis6ExpressionBacterialMutationE54K E110K P242A L372PAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRAM18dSGK[MCS]
Plasmid#84684PurposeCan be used for the transformation of rickettsiaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSC-SmBiT
Plasmid#207632PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiTDepositorInsertphotocaged SpyCatcher-SmBiT
Tags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET246-pUC57 12xMS2V6
Plasmid#104390PurposeMS2V6 cloningDepositorInsert12xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
VRK1_HUMAN_D0
Plasmid#79684PurposeThis plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pReporter_7
Plasmid#60567PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_7
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-Nb127D01-HA-His
Plasmid#171566PurposeBacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)DepositorInsertNb127D01-HA-His (CXCR2 Human)
TagsHA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53SS(1-393)
Plasmid#200311PurposeBacterial expression of human superstable p53 as His6GB1tev fusionDepositorInsertp53 (TP53 Human)
TagsHis6-GB1-tevExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_TKT-Halo
Plasmid#175331PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miniGFP2
Plasmid#188897PurposeExpress 6xHis-Tag miniGFP2 in E.coliDepositorInsertminiGFP2
Tags6xHisExpressionBacterialPromoteraraBADAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA
Plasmid#64983PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSW003-Pc-mNeonGreen
Plasmid#205018PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)DepositorInsertmNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29b-IPP1-His
Plasmid#124137PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-alphaFlincG
Plasmid#19735DepositorInsertalpha-FlincG
UseProtein expressionTagsHisExpressionBacterialAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-betaFlincG
Plasmid#19736DepositorInsertbeta-FlincG
TagsHisExpressionBacterialAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMRE140
Plasmid#118492Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertTagBFP2
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-tRNA-Ser
Plasmid#174318PurposeFor in vitro transcription of mouse tRNAsDepositorInserttRNA-ser
ExpressionBacterialPromoterT7Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM407
Plasmid#248739PurposepPhlF133 driving sfGFP in pNBU2 integrative Bacteroides plasmidDepositorInsertPBfP2E5-RBS1PLP-PhlF-rrnBT1-T7Te-pPhlF133-RBS1PLP-sfGFP-rrnBT2
ExpressionBacterialAvailable SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM403
Plasmid#248736PurposepLacI43 driving sfGFP in pNBU2 integrative Bacteroides plasmidDepositorInsertPBfP2E5-RBS1PLP-LacI-rrnBT1-pLacI43-RBS1PLP-sfGFP-rrnBT2
ExpressionBacterialAvailable SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ1-promoterless-LacZ
Plasmid#191617PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZDepositorInsertlacZ
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MB 45t3 thsS(t3)R-sfGFP_mCherry
Plasmid#232470PurposeOptimized thiosulfate sensor with fluorescent reporter (GFP), constitutive mCherryDepositorInsertsthsS(t3)
thsR
PphsA342
sfGFP
AxeTxe
mCherry
ExpressionBacterialMutationContains the following mutations K286Q, Q350H, I…PromoterJ23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain
Plasmid#228643PurposeBacterial expression of the SARS-CoV-2 nsp3 macrodomain for crystallization of SARS-CoV-2 nsp3 macrodomain in P 4 3DepositorAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pnEA-NpM-HsNot6_AF
Plasmid#148738PurposeBacterial Expression of HsNot6DepositorInsertHsNot6 (CNOT6 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot6-D491A_AF
Plasmid#148659PurposeBacterial Expression of HsNot6-D491ADepositorInsertHsNot6-D491A (CNOT6 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsNot7-D40AE42A_AF
Plasmid#148675PurposeBacterial Expression of HsNot7-D40AE42ADepositorInsertHsNot7-D40AE42A (CNOT7 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-tRNA-Gly
Plasmid#174317PurposeFor in vitro transcription of mouse tRNAsDepositorInserttRNA-gly
ExpressionBacterialPromoterT7Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK1043
Plasmid#8835DepositorInserttobacco etch virus protease, catalytic domain
TagsMBP and polyarginineExpressionBacterialMutationAutolysis-resistant S219V mutantAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHis-BRD4 BD1
Plasmid#196544PurposeExpression of BRD4 Bromodomain (BD1) in E.coliDepositorAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest17_N-myc
Plasmid#234045PurposeBacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage siteDepositorAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP wild type
Plasmid#174075PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
TagsHis-6ExpressionBacterialPromoterT7Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB6c-GFP
Plasmid#35356DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterLlacO-1Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBTR(hCc)
Plasmid#61026PurposeExpresses horse holocytochrome c in E. coliDepositorAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET mCherry LIC cloning vector (u-mCherry)
Plasmid#29769DepositorTypeEmpty backboneTagsmCherryExpressionBacterialPromoterT7Available SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-PDI
Plasmid#202486PurposeExpresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
ExpressionBacterialPromoterEM7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-15b_LC3B
Plasmid#73949PurposeFor recombinant expression of human LC3B/MAP1LC3B in E. coliDepositorAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.D2-NP
Plasmid#134366PurposeNanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.DepositorInsertD2-NP (DRD2 Human)
TagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianPromoterT7Available SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-BioID2-BioID2-HA
Plasmid#176740PurposeExpresses the promiscuous small biotin ligase, BioID2, in astrocytesDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXint129
Plasmid#178208PurposeEncodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.DepositorExpressionBacterialPromoterlacIAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf1-16
Plasmid#198271PurposeUnmodified pPVC; contains WT PVC structural/accessory genesDepositorInsertPVCpnf1-16
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCXMG
Plasmid#204409PurposeExpression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI siteDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromotertacAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry
Plasmid#218860Purpose10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.DepositorInserthGCG-ICAM(1235)-nrxn1b
ExpressionBacterialAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Blue
Plasmid#220782PurposeExpresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertblue chromoprotein (aeBlue)
Tags8xHisExpressionBacterialAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pREDawn-AmpR-MCS
Plasmid#188978PurposeFor red light-induced bacterial expression of gene of interest (AmpR)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL99
Plasmid#78577PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR systemDepositorTypeEmpty backboneUseStreptomyces expressionExpressionBacterialPromoterinducible PnitA-NitR systemAvailable SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT11.R
Plasmid#201003PurposepLv1-pUC18R6KT-miniTn7-Gm-mCherry, GmR, ApRDepositorInsertmCherry
ExpressionBacterialPromoterJ23104Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK19pA-MN
Plasmid#86973PurposeChIC; genomic mapping of chromatin proteinsDepositorInsertprotein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
ExpressionBacterialAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-tdTomato-CNA35
Plasmid#61606Purposebacterial expression of N-terminal fusion protein of CNA35 with tdTomatoDepositorInserttdTomato and CNA35
TagsHis tagExpressionBacterialPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
HAT1
Plasmid#25239PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRE-Tn5-145
Plasmid#118529PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only