29,712 results

1. pDiGi

   Plasmid

   #59323

   Purpose

   Inducible expression of dsRed and GFP in bacteria

   Depositor

   Sophie Helaine, David Holden

   Article

   Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

   Inserts

   EGFP
   dsRed
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad and Plac

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAtetO_6xHis-Bst-LF

   Plasmid

   #145799

   Purpose

   Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

   Depositor

   Andrew Ellington

   Article

   Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

   Insert

   Bst-LF
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   tet PA

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pET31b-T7-Spinach2

   Plasmid

   #79783

   Purpose

   Expresses Spinach2 under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pnEA-vH_His-TEV-SEPT2

   Plasmid

   #174491

   Purpose

   bacterial expression of human SEPT2

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-eGFP-MinD

   Plasmid

   #133622

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

   Depositor

   Petra Schwille

   Article

   Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

   Insert

   EGFP-MinD (minD E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPSV39-ugi

   Plasmid

   #186556

   Purpose

   Broad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   ugi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LacUV5

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBAD-HisD-miniGFP2

   Plasmid

   #188897

   Purpose

   Express 6xHis-Tag miniGFP2 in E.coli

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBHM2403

   Plasmid

   #171687

   Purpose

   pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   CnoCAS9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTHSSe_45

   Plasmid

   #109243

   Purpose

   pSC101 var3 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23105

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPaGE Pyl TAG FliC T248TAG

    Plasmid

    #160089

    Purpose

    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.

    Depositor

    Lital Alfonta

    Article

    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea

    Inserts

    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Threonine 248 to TAG stop-codon.

    Promoter

    Pseudomonas aeruginosa Leu-tRNA native promoter a…

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EPHA7 (2REI)

    Plasmid

    #25148

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    epha7.590.899 (EPHA7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. SHMT2

    Plasmid

    #25479

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SHMT2 (SHMT2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A269T

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pDusk

    Plasmid

    #43795

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHBJT001

    Plasmid

    #225152

    Purpose

    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-51b(+)-Caprola_13

    Plasmid

    #194674

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_13
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDL17_Cas9-His Δcys M1C

    Plasmid

    #206251

    Purpose

    Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    M1C, C80S, C574S

    Promoter

    T7

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HisTEV-cDAXX

    Plasmid

    #197012

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    DAXX
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His Puc (aa 1-393)

    Plasmid

    #52295

    Purpose

    Bacterial expression of His tagged Drosophila Puc fragment

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    Puc (puc Fly)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pKJ0463

    Plasmid

    #228715

    Purpose

    Bacterial expression of epT7 RNAP; for protein production and purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Science 0, eadr6006

    Insert

    Sumo_epT7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T3M/G47A/E643G

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNCS-CyOFP

    Plasmid

    #74278

    Purpose

    Constitutive expression of CyOFP in bacteria

    Depositor

    Michael Lin

    Article

    Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    CyOFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-CPDSalI

    Plasmid

    #38252

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNBU2_repA3

    Plasmid

    #211912

    Purpose

    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

    Depositor

    Adam Arkin

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. His6_MBP_FXR2P_P474S_P493N

    Plasmid

    #198695

    Purpose

    Expression of human FXR2 protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FXR2P (FXR2 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Changed proline 474 to serine and proline 493 asp…

    Promoter

    T7

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. WT A1-LCD +NLS

    Plasmid

    #178658

    Purpose

    Bacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    WT A1-LCD +NLS (HNRNPA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET23b-Casp3-His

    Plasmid

    #11821

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRDV5 containing Sb-loop

    Plasmid

    #132695

    Purpose

    pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Sb_loop including 5' and 3' flanking sequences used for ribosome display
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLG006 (Ec67 retron)

    Plasmid

    #157884

    Purpose

    Expresses the Ec67 retron defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    Ec67 retron
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. His6-TEV-mCerulean3-pBluescript

    Plasmid

    #166757

    Purpose

    For purification of mCerulean3 fluorophore

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    mCerulean3
    

    Use

    Tags

    6x Histidine tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBA630

    Plasmid

    #186241

    Purpose

    RfxCas13d Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-RfxCas13d + crRNA negative control
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTW084b

    Plasmid

    #116894

    Purpose

    Activity-independent SE-PACE AP

    Depositor

    David Liu

    Article

    Wang et al Nat Chem Biol. 2018 Aug 20. pii: 10.1038

    Inserts

    g3N
    434-cI/scFv m3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. JMC044-B12

    Plasmid

    #58752

    Purpose

    bacterial expression of PDB 4NW3

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4NW3 (KMT2A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available since

    Aug. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

32. pOPINF

    Plasmid

    #26042

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    His3C-lacZ
    

    Use

    Tags

    MAHHHHHHSSGLEVL

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MP4

    Plasmid

    #69652

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-T-rnVAPA-1-125

    Plasmid

    #13396

    Depositor

    Axel Brunger

    Article

    Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

    Insert

    VAP-A (Vapa Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

35. pBSV2G_P0826-BB0323-mCherryBb

    Plasmid

    #118244

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    BB0323-mCherry translational fusion under the control of the bb0826 promoter
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTHSSe_47

    Plasmid

    #109245

    Purpose

    pSC101 var5 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCG004

    Plasmid

    #87377

    Purpose

    ORF part assembly entry vector derived from pHT01.

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    GFP dropout part
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pY003 (pFnCpf1_delta Cas)

    Plasmid

    #69974

    Purpose

    Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus without Cas genes
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Cas genes are deleted from locus

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSALECTNK-TEM1(S70A,D179G)/csTEM1

    Plasmid

    #81163

    Purpose

    pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Use

    Tags

    Codon swapped TEM-1 from H26 to W290 and His6x

    Expression

    Bacterial

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    lac promoter

    Available since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHH0103_NR1H2_Hormone-recep

    Plasmid

    #109827

    Purpose

    Protein expression and purification of NR1H2_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR1H2_Hormone-recep (NR1H2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pOPINE

    Plasmid

    #26043

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    His-lacZ-KHHHHHH
    

    Use

    Tags

    KHHHHHH and MAHHHHHHSSG

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSALECT-EcoBam

    Plasmid

    #59705

    Purpose

    Folding reporter with ampicillin resistance as a readout

    Depositor

    Christopher Anderson

    Article

    Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBBRBB-eGFP

    Plasmid

    #32549

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET24a-ncOGT-FL

    Plasmid

    #190821

    Purpose

    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

    Depositor

    Gerald Hart

    Article

    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E.coli

    Promoter

    T7

    Available since

    Jan. 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. His-GST

    Plasmid

    #215169

    Purpose

    Encodes the synthetic glutathionine S-transferase with N-terminal His tag

    Depositor

    Travis Beddoe, Bhuvana Shanbhag

    Insert

    His-GST
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

    Plasmid

    #185713

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed W403 and F753 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pYTK068

    Plasmid

    #65175

    Purpose

    Encodes ConR2 as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConR2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMGA-ptac-sfGFP

    Plasmid

    #139934

    Purpose

    IPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

    Depositor

    Christopher Voigt

    Article

    Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

    Insert

    LacI-Ptac-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMSP3535VA

    Plasmid

    #46887

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PnisA

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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