Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,069 results

Showing: 3601 - 3640 of 32069 results

  1. pET-21a(+)-BMTU UDG-6xHis

    Plasmid
    #172197
    Purpose
    expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions
    Depositor
    Andrea Pauli
    Insert
    BMTU UDG
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    172197-plasmid-map-sequence-id-345439

    Enlarge

  6. pOPS0263

    Plasmid
    #133228
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133228-plasmid-map-sequence-id-276541

    Enlarge

  7. pOPS0262

    Plasmid
    #133227
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133227-plasmid-map-sequence-id-276540

    Enlarge

  9. PPP2R5CA

    Plasmid
    #42369
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PPP2R5CA (PPP2R5C Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pYES2.1-TRP1

    Plasmid
    #11007
    Depositor
    Francisco Malagon
    Insert
    TRP1 (TRP1 Budding Yeast)
    Tags
    V5 and his6
    Expression
    Bacterial and Yeast
    Mutation
    ORF-TRP1 without stop codon
    Available Since
    Dec. 16, 2005
    Availability
    Academic Institutions and Nonprofits only
    11007-plasmid-map-sequence-id-6841

    Enlarge

  15. pKD184

    Plasmid
    #90953
    Purpose
    Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively
    Depositor
    Jeffrey Tabor
    Article
    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi
    Inserts
    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    Expression
    Bacterial
    Promoter
    Bba_J23114, PltetO-1, and PphsA
    Available Since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90953-plasmid-map-sequence-id-178324

    Enlarge

  18. DIRAS2

    Plasmid
    #38819
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DIRAS2 (diras2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. pJL CusS NarX Chimera

    Plasmid
    #225652
    Purpose
    Expresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    CusS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225652-plasmid-map-sequence-id-455039

    Enlarge

  30. pJL NrsS NarX Chimera

    Plasmid
    #225653
    Purpose
    Expresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NrsS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225653-plasmid-map-sequence-id-454026

    Enlarge

  31. pJL RssA NarX Chimera

    Plasmid
    #225654
    Purpose
    Expresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    RssA-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225654-plasmid-map-sequence-id-454025

    Enlarge

  32. pJL VanS NarX Chimera

    Plasmid
    #225655
    Purpose
    Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    VanS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225655-plasmid-map-sequence-id-454023

    Enlarge

  33. pQadd1F

    Plasmid
    #135660
    Purpose
    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Expression
    Bacterial
    Promoter
    pFer
    Available Since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135660-plasmid-map-sequence-id-305687

    Enlarge

  35. mu-FLT3Lv1-pTRIP

    Plasmid
    #163519
    Purpose
    Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells
    Depositor
    Thomas Weber
    Insert
    murine-FLT3L variant 1 (Flt3l Mouse)
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163519-plasmid-map-sequence-id-321571

    Enlarge

  36. pQhsCDtRNA+

    Plasmid
    #86014
    Purpose
    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    LPP and Ptac
    Available Since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86014-plasmid-map-sequence-id-169485

    Enlarge

Showing: 3601 - 3640 of 32069 results