4,932 results

1. pCFD5

   Plasmid

   #73914

   Purpose

   multiplex gRNA plasmid

   Depositor

   Simon Bullock

   Article

   Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

   Insert

   dU6-3:tRNA:gRNA:tRNA:gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6:3

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIDS-LSD1fl

   Plasmid

   #109157

   Purpose

   Encodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
   

   Expression

   Insect

   Promoter

   p10

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJFRC19-13XLexAop2-IVS-myr::GFP

   Plasmid

   #26224

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   myr::GFP
   

   Expression

   Insect

   Available Since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcGP67A-PSMA

   Plasmid

   #11524

   Depositor

   Pamela Bjorkman

   Article

   Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

   Insert

   prostate-specific membrane antigen (FOLH1 Human)
   

   Tags

   His

   Expression

   Insect

   Mutation

   There is a short N-terminal truncation of this pr…

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUAS-PE2-attB

   Plasmid

   #149550

   Purpose

   Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   PE2
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   UAS

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM7

   Plasmid

   #154164

   Purpose

   mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   mCherry
   

   Expression

   Insect

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBPHLWL-LexA

   Plasmid

   #26258

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   LexA
   

   Use

   Drosophila transgenesis

   Expression

   Bacterial and Insect

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAceBAC2-MLG-HMF-LST2

   Plasmid

   #220787

   Purpose

   Expresses N-terminal His10-Myc-FLAG-LST2 in insect cells

   Depositor

   Timm Maier

   Article

   Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

   Insert

   LST2 (ZFYVE28 Human)
   

   Tags

   10xHis-Myc-FLAG

   Expression

   Insect

   Promoter

   p10

   Available Since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcBac1-BacMam-4x-PyOtR

   Plasmid

   #217364

   Purpose

   Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   4 copies of pyrrolysyl-tRNA mutant PyOtR
   

   Use

   Baculoviral

   Expression

   Insect and Mammalian

   Promoter

   U6

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ANO10 (TMEM16KA-c001)

    Plasmid

    #110267

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TMEM16K (ANO10 Human)
    

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLIB-HIS-TEV-scCAK1

    Plasmid

    #177014

    Purpose

    Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
    

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFD6

    Plasmid

    #73915

    Purpose

    tissue-specific multiplex gRNA plasmid

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    :tRNA:gRNA:tRNA:gRNA:tRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAS-DSCP

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnos-Cas9-nos

    Plasmid

    #62208

    Purpose

    Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    cas9
    

    Expression

    Insect

    Promoter

    nanos

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pExpreS2-1-GFP

    Plasmid

    #175363

    Purpose

    ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Insert

    sgGFP
    

    Expression

    Insect

    Mutation

    F64L, S65C, and I167T

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

    Plasmid

    #73164

    Purpose

    multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

    Depositor

    Bruce Edgar

    Article

    Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

    Insert

    mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
    

    Tags

    mRFP1, GFP

    Expression

    Insect

    Mutation

    Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Expression

    Insect

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-NTOM20-LOV2

    Plasmid

    #81009

    Purpose

    Expression of LOV2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Tags

    NTOM20

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV, p10

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBIG1a

    Plasmid

    #80611

    Purpose

    pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTriEx-mCherry-Zdk1

    Plasmid

    #81057

    Purpose

    mammalian expression of Zdk1

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACU2

    Plasmid

    #31223

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUASTattB-degradALFA

    Plasmid

    #219663

    Purpose

    Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    degradALFA
    

    Expression

    Insect

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pQUAST-attB

    Plasmid

    #104880

    Purpose

    For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system

    Depositor

    Christopher Potter

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p4X-UASTattB

    Plasmid

    #189858

    Purpose

    Fly cloning and transformation

    Depositor

    Jung Hwan Kim

    Article

    Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RP156 pUAST EGFP:Gr21a

    Plasmid

    #59524

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Gr21 (Gr21a Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

25. RP125 pUAST EGFP:Orco

    Plasmid

    #53441

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Orco

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Orco (Orco Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

26. RP185 pUAST EGFP:Or47b

    Plasmid

    #59525

    Purpose

    N-terminal fusion of GFP to Drosophila melanogaster Or47b

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Or47b (Or47b Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

27. RP132 pUAST EGFP:Or43a

    Plasmid

    #59523

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Or43a

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Or43a (Or43a Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pLIB

    Plasmid

    #80610

    Purpose

    pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pF1-Hs Cdk1/cyclin B1

    Plasmid

    #177330

    Purpose

    Co-expression of the human active Cdk1/cyclin B1 kinase complex

    Depositor

    Andreas Boland

    Article

    Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

    Inserts

    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    

    Tags

    2 x Strep-tag and 8 x His

    Expression

    Insect

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)

    Plasmid

    #231721

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    POLR2A (POLR2A Human)
    

    Tags

    Flag-GST-3C

    Expression

    Insect

    Mutation

    RPB1:1586-1971

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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31. pBIG1b

    Plasmid

    #80612

    Purpose

    pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAc-C Med12His

    Plasmid

    #49240

    Purpose

    expresses human Med12 with His tag in insect cells

    Depositor

    Dylan Taatjes

    Article

    Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

    Insert

    Med12 (MED12 Human)
    

    Tags

    His

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBIG2ab

    Plasmid

    #80616

    Purpose

    pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFL-EGFP-3xFlag-HLTF

    Plasmid

    #193055

    Purpose

    Baculovirus expression of human HLTF for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    HLTF (HLTF Human)
    

    Tags

    3xFlag

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBIG2abc

    Plasmid

    #80617

    Purpose

    pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBIG2abcde

    Plasmid

    #80619

    Purpose

    pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBIG2abcd

    Plasmid

    #80618

    Purpose

    pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pBIG1c

    Plasmid

    #80613

    Purpose

    pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBIG1d

    Plasmid

    #80614

    Purpose

    pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBIG1e

    Plasmid

    #80615

    Purpose

    pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge