76,374 results

1. pLenti-mCherry-Mango II x 24

   Plasmid

   #127587

   Purpose

   Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

   Depositor

   David Rueda

   Article

   Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

   Insert

   mCherry-Mango II x24
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE Tet ON

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. plenti6-SLC7A11/xCT-V5

   Plasmid

   #170427

   Purpose

   Eepresses SLC7A11 (also named xCT) in mammalian cells

   Depositor

   Boyi Gan

   Article

   Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

   Insert

   solute carrier family 7 member 11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. qTAG-C-dTAG-Zeo

   Plasmid

   #207738

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. ER-GCaMP6f

   Plasmid

   #182548

   Purpose

   Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

   Depositor

   Chris Richards

   Article

   Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

   Insert

   ER localized P450
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCW-SPI1

   Plasmid

   #162824

   Purpose

   Doxycycline-inducible overexpression of human SPI1

   Depositor

   Matthew Steinhauser

   Article

   Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet ON

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-EF1a-eFFly-mCherry

   Plasmid

   #104833

   Purpose

   3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   eFFly
   mCherry
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmStayGold_mStayGold_I3

   Plasmid

   #239339

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

   Depositor

   Torsten Wittmann

   Article

   Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

   Insert

   I3-01
   

   Use

   Tags

   mStayGold

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available since

   June 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-BRD4ΔN-mCherry-sspB

   Plasmid

   #121968

   Purpose

   Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-461

   Promoter

   SFFV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLIX403_UBAP2L_mCherry

   Plasmid

   #105286

   Purpose

   puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   UBAP2L (UBAP2L Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CROPseq-multi-Puro (pRW1441)

    Plasmid

    #216217

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ABE9

    Plasmid

    #194208

    Purpose

    Mammalian expression of ABE9

    Depositor

    Dali Li

    Article

    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

    Insert

    TadA-8e (N108Q/L145T)-nSpCas9
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FLAG-SKP2

    Plasmid

    #215092

    Purpose

    Expresses FLAG-SKP2 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    SKP2 (SKP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid

    #192786

    Purpose

    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNGR1 (IFNGR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence and mXFPm

    Expression

    Mammalian

    Mutation

    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pORANGE GFP-Grin2a KI

    Plasmid

    #131486

    Purpose

    Endogenous tagging of GluN2a: N-terminal (amino acid position: A25)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNANOG-Luc

    Plasmid

    #25900

    Depositor

    Ren-he Xu

    Article

    Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

    Insert

    NANOG promoter (NANOG Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1 GFP shRNA

    Plasmid

    #30323

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    GFP shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pORANGE GFP-Grin2b KI

    Plasmid

    #131487

    Purpose

    Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Grin2b Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-TO-NFIA.1-SOX9

    Plasmid

    #182310

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP VAMP7 (1-120)

    Plasmid

    #42317

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Longin domain (1-120) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ120-220

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pLenti_CMV:Flag-Antares

    Plasmid

    #208852

    Purpose

    Expresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    Antares
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMito-mCherry-FRB_IRES_FKBP(III)-GBPen

    Plasmid

    #128268

    Purpose

    Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).

    Depositor

    Stephen Royle

    Article

    Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

    Inserts

    MitoTrap
    FKBP(III)-GBPen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SBE-NLS-mCherry PGK-rtTA3

    Plasmid

    #195332

    Purpose

    Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Inserts

    NLS-mCherry
    rtTA3
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and miniCMV

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3_cAMPFIRE-H

    Plasmid

    #182281

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-H (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP VAMP7 (102-220)

    Plasmid

    #42314

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ1-102

    Promoter

    CMV

    Available since

    March 13, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pEGFP-C1-NuMA

    Plasmid

    #81029

    Purpose

    Expresses human NuMA with N-terminal EGFP tag in mammalian cells

    Depositor

    Michael Mancini

    Insert

    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

    Plasmid

    #154139

    Purpose

    Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
    

    Use

    Tags

    3x FLAG and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti6.2_miRFP670

    Plasmid

    #113726

    Purpose

    Expresses the fluorescent protein miRFP670 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    miRFP670
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. qTAG-N-Puro-3xHA

    Plasmid

    #207696

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pCBASceI

    Plasmid

    #26477

    Purpose

    I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

    Depositor

    Maria Jasin

    Article

    Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

    Insert

    pCBASceI
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLIX403_deltaUBA_UBAP2L_mCherry

    Plasmid

    #105287

    Purpose

    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    UBAP2L (UBAP2L Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deleted amino acids 20-108

    Promoter

    TRE promoter, Tet ON

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLIX403 RNaseH1 Catalytic Inactive

    Plasmid

    #237796

    Purpose

    Inducible lentiviral expression of RNaseH1 Catalytic Inactive

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    RNaseH1 (RNASEH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D210N

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HA-Halotag-FRB-EX-nes_pLX304

    Plasmid

    #120913

    Purpose

    Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    HA-Halotag-FRB-EX-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRK5-HA-Ubiquitin-K48R

    Plasmid

    #17604

    Purpose

    Mammalian expression of HA tagged ubiquitin K48R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48R (expected to disrupt G76-K48 ubiquitin chain…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti PGK GFP Blast (w510-5)

    Plasmid

    #19069

    Purpose

    3rd gen lentiviral eGFP expression vector, PGK promoter, Blast

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJC49

    Plasmid

    #133060

    Purpose

    Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GFP-C1-CAMKIIalpha

    Plasmid

    #21226

    Depositor

    Tobias Meyer

    Article

    Shen et al Neuron. 1998 Sep . 21(3):593-606.

    Insert

    CAMKIIalpha (Camk2a Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lab plasmid KS 0001.

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHTN_HaloTag_KIF5B

    Plasmid

    #213404

    Purpose

    Full-length human kinesin-1 with HaloTag at the N-terminus

    Depositor

    Jonas Ries

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    HaloTag-KIF5B (KIF5B Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge