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81,074 results

Showing: 10561 - 10590 of 81074 results

  1. pAAV-CAG-SomaGCaMP6f2

    Plasmid
    #158757
    Purpose
    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has Service
    AAV8 and AAV9
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158757-plasmid-map-sequence-id-310662

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  3. pSCMV-H2B-FT-Medium

    Plasmid
    #157670
    Purpose
    Retroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
    Depositor
    Shangqin Guo
    Article
    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi
    Insert
    Histone H2B
    Use
    Retroviral
    Tags
    Medium-FT
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157670-plasmid-map-sequence-id-308113

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  5. pcDNA3.1.D2-NP

    Plasmid
    #134366
    Purpose
    Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    D2-NP (DRD2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134366-plasmid-map-sequence-id-306391

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  6. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid
    #140981
    Purpose
    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasL-RLuc8 (GNAS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140981-plasmid-map-sequence-id-300681

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  7. pcDNA3.1-Beta1

    Plasmid
    #140987
    Purpose
    Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GBeta1 (GNB1 Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal human rhinovirus (HRV) 3C …
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140987-plasmid-map-sequence-id-301588

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  10. CMV-eGFP-Z1

    Plasmid
    #135049
    Purpose
    Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135049-plasmid-map-sequence-id-269408

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  21. pAAV-Ef1a-fDIO-JRGECO1a

    Plasmid
    #128317
    Purpose
    Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.
    Depositor
    Patricia Jensen
    Has Service
    AAV1
    Insert
    NES-JRGECO1a
    Use
    AAV
    Tags
    His Tag
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128317-plasmid-map-sequence-id-257202

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  22. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Synthetic, Human)
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

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Showing: 10561 - 10590 of 81074 results