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Showing: 101 - 150 of 1735 results

  1. pJK2014

    Plasmid
    #171931
    Purpose
    To express sygl-1 fused to 24 copies of MS2 loops.
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Psygl-1::24XMS2 loops::3X Flag::sygl-1 ORF::sygl-1 3'UTR
    Use
    Tags
    N-terminal Tag 24X MS2 loops
    Expression
    Worm
    Mutation
    Promoter
    -
    Available since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171931-plasmid-map-sequence-id-337176

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  11. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163094-plasmid-map-sequence-id-320582

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  12. pJH4203

    Plasmid
    #200160
    Purpose
    Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFP
    Depositor
    Mei Zhen
    Article
    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do
    Insert
    twk-40 (twk-40 Nematode)
    Use
    Tags
    GFP
    Expression
    Worm
    Mutation
    Promoter
    Prig-3
    Available since
    May 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    200160-plasmid-map-sequence-id-413719

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  16. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid
    #107939
    Purpose
    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.
    Depositor
    Heng-Chi Lee
    Article
    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d
    Insert
    mCherry(dpiRNA)::ANI-1(AHPH)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    pie-1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107939-plasmid-map-sequence-id-207466

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  18. pJJR83

    Plasmid
    #75028
    Purpose
    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Article
    Ramalho et al Development. 2020 Jul 22;147(14):dev1880
    Insert
    mCherry^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized mCherry
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75028-plasmid-map-sequence-id-141918

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  19. pJW2171

    Plasmid
    #163095
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163095-plasmid-map-sequence-id-320590

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  21. pJL046

    Plasmid
    #198807
    Purpose
    In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198807-plasmid-map-sequence-id-390993

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  27. pCZGY2850

    Plasmid
    #87758
    Purpose
    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.
    Depositor
    Andrew Chisholm
    Article
    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10
    Insert
    Pcol-19-PH-miniSOG(Q103L)
    Use
    Tags
    PH
    Expression
    Worm
    Mutation
    Q103L
    Promoter
    Pcol-19
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    87758-plasmid-map-sequence-id-174819

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  34. pPD95_77

    Plasmid
    #1495
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, rf2204, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1495-plasmid-map-sequence-id-106

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  35. pJM483

    Plasmid
    #52987
    Purpose
    A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor
    Depositor
    Ding Xue
    Article
    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d
    Insert
    Lactaherin (Mfge8 Mouse)
    Use
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    52987-plasmid-map-sequence-id-82563

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  38. L4417

    Plasmid
    #1649
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    RNAi
    Tags
    T7p, gfpC, gfpS65S, gfpN, T7p, lacZN, OriF1>&g…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1649-plasmid-map-sequence-id-259

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  39. pDD284

    Plasmid
    #66825
    Purpose
    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. pJR021

    Plasmid
    #198814
    Purpose
    Variant of yellow fluorescent protein for transient gene expression
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::destabilizedYFP::let-838 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    YFP with destabilized signal from mouse ornithine…
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198814-plasmid-map-sequence-id-391694

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  50. pAS1-rab3P::his-24::mNeptune

    Plasmid
    #124350
    Purpose
    Nuclear Localized Pan-Neuronal mNeptune
    Depositor
    Andrew Leifer
    Insert
    rab-3, mNeptune (rab-3 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124350-plasmid-map-sequence-id-241971

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Showing: 101 - 150 of 1735 results