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Synthetic Biology

11,729 results

Showing: 1 - 50 of 11729 results

  1. pHREAC

    Plasmid
    #134908
    Purpose
    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.
    Depositor
    George Lomonossoff
    Article
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    double 35S
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134908-plasmid-map-sequence-id-342556

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  4. TETv4

    Plasmid
    #167983
    Purpose
    Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    TETv4 (TET1CD-XTEN80-dCas9)
    Use
    CRISPR and Synthetic Biology
    Tags
    3xNLS, TET1 catalytic domain, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167983-plasmid-map-sequence-id-418937

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  9. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  11. pAAV.hSyn.iGluSnFR3.v857.GPI

    Plasmid
    #178331
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has Service
    AAV1
    Insert
    pAAV hSyn iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178331-plasmid-map-sequence-id-361545

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  12. pOpen-GBDpol

    Plasmid
    #165501
    Purpose
    Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165501-plasmid-map-sequence-id-338429

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  19. pJAB988

    Plasmid
    #133920
    Purpose
    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter
    Depositor
    Christopher Voigt
    Article
    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.
    Insert
    miniICE-tet(M)cat(PC194)_PT7-GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    133920-plasmid-map-sequence-id-409802

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  22. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165563-plasmid-map-sequence-id-340036

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  23. pMP416

    Plasmid
    #134996
    Purpose
    dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Use
    Synthetic Biology
    Tags
    3xFLAG, E coli Dam(N132A), and dCas9
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134996-plasmid-map-sequence-id-267931

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  24. pOpen-T4 g32

    Plasmid
    #165560
    Purpose
    A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4 gene 32
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165560-plasmid-map-sequence-id-339953

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  26. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid
    #55201
    Purpose
    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55201-plasmid-map-sequence-id-240663

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  27. pOpen-MMLV_RT (mut H)

    Plasmid
    #165546
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165546-plasmid-map-sequence-id-339423

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  28. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid
    #227246
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227246-plasmid-map-sequence-id-450569

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  29. pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

    Plasmid
    #223287
    Purpose
    A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.
    Depositor
    Jared Toettcher
    Article
    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860
    Insert
    myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
    Use
    Synthetic Biology; Piggybac transposase insertabl…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    223287-plasmid-map-sequence-id-442312

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  31. pcDNA3.3-Nm-dCas9-p300 Core

    Plasmid
    #61365
    Purpose
    encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    Nm-dCas9-p300 Core (EP300 Neisseria meningiditis, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61365-plasmid-map-sequence-id-244292

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  35. pTrig

    Plasmid
    #104576
    Purpose
    Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under Lac promoter for copy increase.
    Depositor
    Harris Wang
    Article
    Sheth et al Science. 2017 Dec 15;358(6369):1457-1461
    Insert
    PLac-repL
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    104576-plasmid-map-sequence-id-201435

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  39. TRAV29/DV5

    Plasmid
    #237986
    Purpose
    Encodes TRAV29/DV5 allele, P2A, and TRBC to generate TCRs via cloning
    Depositor
    Michael Birnbaum
    Insert
    TRBC-P2A-TRAV29/DV5 (TRAV29DV5 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237986-plasmid-map-sequence-id-475854

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  40. TRAV26-2

    Plasmid
    #237984
    Purpose
    Encodes TRAV26-2 allele, P2A, and TRBC to generate TCRs via cloning
    Depositor
    Michael Birnbaum
    Insert
    TRBC-P2A-TRAV26-2 (TRAV26-2 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237984-plasmid-map-sequence-id-475853

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  41. TRBV11-2

    Plasmid
    #237933
    Purpose
    Encodes TRBV11-2 allele and TRAC to generate TCRs via cloning
    Depositor
    Michael Birnbaum
    Insert
    TRBV11-2-TRAC (TRBV11-2 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237933-plasmid-map-sequence-id-475850

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  42. TRAV14/DV4

    Plasmid
    #237972
    Purpose
    Encodes TRAV14/DV4 allele, P2A, and TRBC to generate TCRs via cloning
    Depositor
    Michael Birnbaum
    Insert
    TRBC-P2A-TRAV14/DV4 (TRAV14DV4 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237972-plasmid-map-sequence-id-475843

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  43. TRAV30

    Plasmid
    #237987
    Purpose
    Encodes TRAV30 allele, P2A, and TRBC to generate TCRs via cloning
    Depositor
    Michael Birnbaum
    Insert
    TRBC-P2A-TRAV30 (TRAV30 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237987-plasmid-map-sequence-id-475832

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  44. TRBV4-3

    Plasmid
    #237907
    Purpose
    Encodes TRBV4-3 allele and TRAC to generate TCRs via cloning
    Depositor
    Michael Birnbaum
    Insert
    TRBV4-3-TRAC (TRBV4-3 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    237907-plasmid-map-sequence-id-475797

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  46. pDule2-Mb haloTyrRS C6

    Plasmid
    #160378
    Purpose
    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.
    Depositor
    Ryan Mehl
    Article
    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574
    Insert
    C6 HaloTyrosine tRNA synthatase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    L270S, Y271L, N311G, C313T
    Promoter
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    160378-plasmid-map-sequence-id-315318

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  47. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  49. pUC19-T7-3WJdB-T

    Plasmid
    #87308
    Purpose
    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Three-way junction dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87308-plasmid-map-sequence-id-174739

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Showing: 1 - 50 of 11729 results