4,075 results

1. pcDNA 3.1(-) mouse C/EBP delta

   Plasmid

   #12559

   Depositor

   Peter Johnson

   Article

   McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

   Insert

   CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S2G introduced during cloning

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGFP-Rab8A[T22N]

   Plasmid

   #24899

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   hRab8A[T22N]
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 22 to Asparagine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2-hDKK1-flag

   Plasmid

   #15494

   Depositor

   Sergei Sokol

   Article

   Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

   Insert

   DKK1 (DKK1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pAlbumin

   Plasmid

   #22037

   Depositor

   Didier Trono

   Insert

   partial sequence of human albumine
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMXs-hNANOG (Plath)

   Plasmid

   #18115

   Depositor

   Kathrin Plath

   Article

   Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

   Insert

   Nanog homeobox (NANOG Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC-GoldyTALEN

   Plasmid

   #38143

   Purpose

   destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

   Depositor

   Daniel Carlson

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

   Insert

   GoldyTALEN
   

   Use

   Tags

   AcV5 and FokI homodimer

   Expression

   Mammalian

   Mutation

   Truncate at N and C terminous

   Promoter

   miniCaggs

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-mPIAS3

   Plasmid

   #15207

   Depositor

   Ke Shuai

   Article

   Chung et al Science. 1997 Dec 5. 278(5344):1803-5.

   Insert

   PIAS3 (Pias3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V580I

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SRa-HA-SUMO2 (Sentrin 3)

   Plasmid

   #17360

   Purpose

   Mammalian expression of human SUMO2 with HA tag

   Depositor

   Edward Yeh

   Article

   Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

   Insert

   SUMO2 (SUMO2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTA-Luc SIRT1 promoter (-202)

   Plasmid

   #10971

   Depositor

   Toren Finkel

   Article

   Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

   Insert

   SIRT1 promoter (Sirt1 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmCherry-CRY2-CreN

    Plasmid

    #26888

    Purpose

    Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2-CreN (cre, CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 19-104 of Cre

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Stat3 S727A pRc/CMV

    Plasmid

    #8708

    Depositor

    Jim Darnell

    Article

    Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

    Insert

    Stat3 S727A (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S727A (exhibits o serine phosphorylation either c…

    Promoter

    Available since

    Aug. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 1099 pcDNA GFP FKHR AAA

    Plasmid

    #9023

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLM-vexGFP-Oct4

    Plasmid

    #22240

    Purpose

    2nd generation lentiviral vector

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    vexGFP_2A_OCT4 (POU5F1 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJL44 - Phsp16.48::MosTase::glh-2utr

    Plasmid

    #19333

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Phsp-16::Mos1 transposase
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV-LTR-dCas9-p65AD-BFP

    Plasmid

    #46913

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-p65AD-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK1035

    Plasmid

    #8832

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    TVMV protease
    

    Use

    Tags

    His and MBP (cleaved)

    Expression

    Bacterial

    Mutation

    synthetic gene with codons optimized for E. coli

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLOVE-Nmyc

    Plasmid

    #15951

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

    Insert

    v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Promoter

    Available since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

18. TetO-FUW-oct4

    Plasmid

    #20323

    Depositor

    Rudolf Jaenisch

    Article

    Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

    Insert

    oct4 (Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAL175

    Plasmid

    #22276

    Purpose

    Expresses YFP-PIF for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-Oct3/4-IP

    Plasmid

    #15918

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pST1374

    Plasmid

    #13426

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMLM292

    Plasmid

    #21873

    Depositor

    Keith Joung

    Article

    Foley et al Nat Protoc. 2009. 4(12):1855-67.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMLM290

    Plasmid

    #21872

    Depositor

    Keith Joung

    Article

    Foley et al Nat Protoc. 2009. 4(12):1855-67.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5 DN-Fyn

    Plasmid

    #16033

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dominant negative: K299M

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pMKO.1 puro PTEN shRNA

    Plasmid

    #10669

    Depositor

    William Hahn

    Insert

    PTEN shRNA (PTEN Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5-myc-Rac1-wt

    Plasmid

    #12985

    Depositor

    Gary Bokoch

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBS aP2 promoter (5.4kb) polyA

    Plasmid

    #11424

    Depositor

    Ronald Kahn

    Insert

    aP2 promoter (Fabp4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS2TAL3-DD

    Plasmid

    #37275

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    Flag, Fok I DD, Tal-C, and Tal-N

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTSA-13

    Plasmid

    #17327

    Depositor

    Takeshi Sano

    Article

    Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

    Insert

    streptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated to include only amino acids 16-133 of m…

    Promoter

    Available since

    Feb. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAC-Kan-alphaGal4

    Plasmid

    #13421

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    RNAP alpha-Gal4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCS2TAL3-RR

    Plasmid

    #37276

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEM-Sox2

    Plasmid

    #16353

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJW1219

    Plasmid

    #61250

    Purpose

    CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 902 pLNCX myr HA Akt1 K179M

    Plasmid

    #9006

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 K179M (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    K179M. kinase dead.

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. hAur-A

    Plasmid

    #8510

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hAur-A (AURKA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTriEx-RhoA FLARE.sc Biosensor Q63L

    Plasmid

    #12151

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor Q63L (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA Q63L (dominant-positive mutation)

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RCIscript-GoldyTALEN

    Plasmid

    #38142

    Purpose

    destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

    Depositor

    Daniel Carlson, Stephen Ekker

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Mrna transcription vector

    Tags

    AcV5 and FokI homodimer

    Expression

    Mutation

    Truncate at N and C terminous

    Promoter

    T3

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBabe-Puro-RhoA FLARE.sc Biosensor

    Plasmid

    #12602

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSico PGK GFP

    Plasmid

    #12093

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

    Plasmid

    #35499

    Purpose

    AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Has Service

    AAV9

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    CaMKIIa

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge