We narrowed to 6,925 results for: CAD

1. GB1-PRD(703-800)-Strep

   Plasmid

   #141344

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

   Insert

   GB1-His6-TEV-ALIX(703-800)-Strep
   

   Use

   Tags

   GB1-His6-TEV and Strep-tag (WSHPQFEK)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mEos3-Rac1

   Plasmid

   #92039

   Purpose

   Expresses mEos3-Rac1 fusion protein

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBabe puro-HA-PIK3CA(H1047R;G320E)

   Plasmid

   #33254

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and glycine 32…

   Promoter

   SV40

   Available since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

4. SunTagCACTA1g2-22aa-TET1cd

   Plasmid

   #106437

   Purpose

   SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

   Depositor

   Steven Jacobsen

   Article

   Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

   Insert

   CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSc-His6-Sem1Sc

   Plasmid

   #83235

   Purpose

   pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   Sem 1 (SEM1 Budding Yeast)
   

   Use

   Tags

   His6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PV225

   Plasmid

   #132334

   Purpose

   dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
   

   Use

   Tags

   AT-CBF1

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMSCV-PIG-Lin28b

   Plasmid

   #64795

   Purpose

   Expresses human Lin28b in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Lin28b (LIN28B Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mEos3-dnRac1

   Plasmid

   #92040

   Purpose

   Expresses mEos3 fusion with dnRac1 (T17N).

   Depositor

   Ji Yu

   Article

   Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Tags

   mEos3

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T17N (dominant negative)

   Promoter

   CMV

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MO70-hJunctate

   Plasmid

   #79596

   Purpose

   Transient and retroviral expression

   Depositor

   Yousang Gwack

   Article

   Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

   Insert

   junctate (ASPH Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pDN-G1Gh

    Plasmid

    #44507

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    yEGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pISH-Ascl1

    Plasmid

    #74338

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Ascl1 (also known as Mash1). Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

    Insert

    achaete-scute family bHLH transcription factor 1 (Ascl1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DICER KO vector

    Plasmid

    #16656

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

    Insert

    Dicer knock-out (DICER1 Human)
    

    Use

    Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pMXs-ms-c-Myc-V394D

    Plasmid

    #50775

    Purpose

    retroviral expression of mouse c-Myc V394D mutant

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    c-Myc V394D (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V394D (does not bind to Miz-1)

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA1-Bystin

    Plasmid

    #11869

    Depositor

    Michiko Fukuda

    Article

    Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

    Insert

    Bystin (BYSL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-PM-InPAkt

    Plasmid

    #181915

    Purpose

    Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

    Insert

    pm-InPAkt
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Contains the following mutations with respect to …

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBRPyCAG-fmKlf4-DsRed-Ip

    Plasmid

    #26274

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28_6xHis-PARP1delWGR-L713F

    Plasmid

    #173941

    Purpose

    Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI-Neo-gp78 R2M / JM21

    Plasmid

    #13304

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C356G ; H362A

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac-CHD7 (787-1899)

    Plasmid

    #170145

    Purpose

    Expresses residues 787-1899 of CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    N-terminal truncation of residues 1-786; C-termin…

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFastBac-CHD7 (787-2138)

    Plasmid

    #170146

    Purpose

    Expresses residues 787-2138 of CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    N-terminal truncation of residues 1-786; C-termin…

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFastBac-CHD7 (936-2997)

    Plasmid

    #170147

    Purpose

    Expresses residues 936-2997 of CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    N-terminal truncation of residues 1-935

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDNA3-HA-HA-human CMYC (1-170)

    Plasmid

    #203426

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA3-HA-HA-human CMYC (1-208)

    Plasmid

    #203427

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3-HA-HA-human CMYC (1-277)

    Plasmid

    #203428

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3-HA-HA-human CMYC (170-439)

    Plasmid

    #203430

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMKO shRNA Bad

    Plasmid

    #17237

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bad shRNA (BAD Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

28. J23117-mRFP1-331Bb

    Plasmid

    #78280

    Purpose

    mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23117-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HOXB7 (human) pCMV

    Plasmid

    #8539

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pLKO-1: shALAS-1

    Plasmid

    #22750

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shALAS-1 (Alas1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pCMV10-Flag-Fbxl3

    Plasmid

    #48751

    Purpose

    Expression of FLAG-tagged Fbxl3

    Depositor

    Joseph Takahashi

    Article

    Siepka et al Cell. 2007 Jun 1;129(5):1011-23. Epub 20

    Insert

    Fbxl3 (Fbxl3 Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

32. pDONR221-Ctdnep1_Cter

    Plasmid

    #196522

    Purpose

    Entry vector for Gateway with Ctdnep1_Cter

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1-Cter (CTDNEP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No transmembrane domain

    Promoter

    CMV promoter; T7 promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR201-Ctdnep1

    Plasmid

    #196520

    Purpose

    Entry vector for Gateway with Ctdnep1

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1-N1 (CTDNEP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCEP4-myc-HLA-A2-VN

    Plasmid

    #135485

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. nuc-yHS1-M7A

    Plasmid

    #159167

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1-M7A
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pAdTrack: shRev-Erbalpha

    Plasmid

    #22749

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Adenoviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

37. HOXB4 (human) FLAG MSCV

    Plasmid

    #8528

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Missing N-terminal 14 amino acids; improved Ribos…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

38. MAP4K3-GFP K45E

    Plasmid

    #21734

    Depositor

    L. Miguel Martins

    Article

    Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

    Insert

    MAP4K3 (MAP4K3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of the ATP-binding site (K45E). This ver…

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

39. MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

    Plasmid

    #19746

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 and PTEN (Pten Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTSlb-Q758C

    Plasmid

    #60729

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMXs-hu-c-Myc-dN2

    Plasmid

    #50774

    Purpose

    retroviral expression of human c-Myc dN2 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    c-Myc -dN2 (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 42-439

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. p6613 MSCV-IP N-HAonly RBCK1

    Plasmid

    #35011

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    RBCK1 (RBCK1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

43. pMXs-hu-c-Myc-dN1

    Plasmid

    #50773

    Purpose

    retroviral expression of human c-Myc dN1 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    c-Myc -dN1 (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 14-439

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCNL-C1_fibrillarin

    Plasmid

    #65709

    Purpose

    Expresses CNL-fibrillarin in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    fibrillarin (FBL Human)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

45. pCI-neo-(HA)3-ε

    Plasmid

    #198531

    Purpose

    Expression of HA-tagged AP-4 ε in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

    Insert

    AP-4 ε (AP4E1 Human)
    

    Use

    Tags

    triple HA tag

    Expression

    Mammalian

    Mutation

    silent substitutions in codons 235, 262, 905 and …

    Promoter

    CMV

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pOTTC1618 pAAV SYN1 Nuc-EYFP miR-30 FF3

    Plasmid

    #135564

    Purpose

    An AAV vector expressing miR-30a shRNA vs FF luciferase and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    miR-30 FF3
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    SYN1 and hSYN1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pGEX-2TK cyclin T1 (1-250) (P#467)

    Plasmid

    #14606

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-250) (CCNT1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-250

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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48. HOXB4 (human) HA-TAT-tag pET

    Plasmid

    #8526

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    replace T7 and HIS tags of pET with an HA tag;

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

49. pDN-G1Tt

    Plasmid

    #44509

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    tetR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET28_6xHis-PARP1-A774L

    Plasmid

    #174796

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A774L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A774L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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