We narrowed to 11,958 results for: pcDNA

1. pcDNA5/FRT/TO_Lyn11-HaloTag7

   Plasmid

   #175533

   Purpose

   Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lyn11-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA5/FRT-ChemoG-ATP

   Plasmid

   #193818

   Purpose

   CMV overexpression of ChemoG-ATP in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA4-PKCZeta WT His tagged

   Plasmid

   #24609

   Depositor

   Jeff Wrana

   Article

   Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

   Insert

   PKCZeta (PRKCZ Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. mCherry-2A-eGFP(W58X) pcDNA3.1(-)

   Plasmid

   #172229

   Purpose

   Expresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cells

   Depositor

   Joshua Rosenthal

   Article

   Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

   Insert

   mCherry-2A-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-MS2-HA-HsStaufen2-T1_V

   Plasmid

   #147741

   Purpose

   Mammalian Expression of HsStaufen2-T1

   Depositor

   Elisa Izaurralde

   Insert

   HsStaufen2-T1 (STAU2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 2xFLAG-2xSTREP TFDP2

   Plasmid

   #236445

   Purpose

   transient overexpression of TFDP2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   TFDP2 (TFDP2 Human)
   

   Use

   Tags

   FLAG-FLAG-STREP-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3 2xFLAG-2xSTREP TFDP1

   Plasmid

   #236444

   Purpose

   transient overexpression of TFDP1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   TFDP1 (TFDP1 Human)
   

   Use

   Tags

   FLAG-FLAG-STREP-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 2xFLAG-2xSTREP E2F6

   Plasmid

   #236441

   Purpose

   transient overexpression of E2F6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   E2F6 (E2F6 Human)
   

   Use

   Tags

   FLAG-FLAG-STREP-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 2xFLAG-2xSTREP E2F5

   Plasmid

   #236440

   Purpose

   transient overexpression of E2F5 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   E2F5 (E2F5 Human)
   

   Use

   Tags

   FLAG-FLAG-STREP-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-hRIPK1-Flag-delC

    Plasmid

    #112493

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of C terminus (AA290-671 deleted)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO GFP DNAJA2

    Plasmid

    #19492

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJA2 (DNAJA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-smURFP-StagRFP-EKAR-EV

    Plasmid

    #173019

    Purpose

    Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    smURFP-StagRFP-EKAR-EV
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.1- dCas9-2xNLS-EGFP

    Plasmid

    #74710

    Purpose

    Expresses dCas9-2xNLS-EGFP in mammalian cells

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    dCas9-2xNLS-EGFP
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO_Lyn11-HaloTag9

    Plasmid

    #175543

    Purpose

    Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-myc-BirA*-KRASG12V

    Plasmid

    #122208

    Purpose

    Expresses myc-BirA* fused to KRASG12V in mammalian cells

    Depositor

    Christopher Counter

    Article

    Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

    Insert

    KRASG12V (KRAS Human)
    

    Use

    Tags

    myc-BirA*

    Expression

    Mammalian

    Mutation

    changed G12 to V

    Promoter

    CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 Lats1 (Nigg HS189)

    Plasmid

    #41156

    Depositor

    Erich Nigg

    Article

    Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

    Insert

    Lats1 (LATS1 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5-FRT/T0-Flag-BRIP1

    Plasmid

    #222619

    Purpose

    Mammalian expression vector of flag-tagged BRIP1

    Depositor

    Daniel Durocher

    Article

    Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

    Insert

    BRIP1 (BRIP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pcDNA3-MAVS-invitro(KaganD86)

    Plasmid

    #52012

    Purpose

    Used for in vitro expression of the human MAVS CDS

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA4 CoV-2 nsp1 3xFLAG

    Plasmid

    #176057

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1+-mutTornado-split-nLuc

    Plasmid

    #212707

    Purpose

    Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    mutTornado-CVB3-split-nLuc
    

    Use

    Tags

    Expression

    Mutation

    Deleted the partial 3'ribozyme sequence in T…

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-dCas9-MQ1(WT)-EGFP

    Plasmid

    #89633

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG and 6*His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

    Plasmid

    #194678

    Purpose

    CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    CHRM3_cpHalo∆
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-CMV-PdCO-mScarlet

    Plasmid

    #198507

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5-FRT-TO-NOD1-EGFP

    Plasmid

    #131206

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD1 (NOD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5 FRT/TO Plk4 ∆24-mCherry

    Plasmid

    #80271

    Purpose

    mammalian expression plasmid for Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5-EGFP-AID-BubR1

    Plasmid

    #47330

    Purpose

    for plasmid integration using the Flp-In system. AID at N-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Use

    Tags

    AID and GFP

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Promoter

    Available since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-CibN-dCas9-CibN

    Plasmid

    #60553

    Purpose

    Expresses CibN-dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 5'-3xFLAG minimal CMV pCDNA5

    Plasmid

    #212044

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-DRD2-cpHalo∆-mEGFP

    Plasmid

    #194679

    Purpose

    CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    DRD2_cpHalo∆
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1/Puro-CAG-JEDI-2P

    Plasmid

    #179458

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1/Myc-His beta4

    Plasmid

    #16039

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-FKBP-ICUE3-NLS

    Plasmid

    #138255

    Purpose

    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    FKBP-ICUE3-NLS
    

    Use

    Tags

    FKBP, Nuclear localization signal (NLS), cpVenus[…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1-mas-KB1691-Nluc

    Plasmid

    #204324

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    KB1691-Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pcDNA3-FLAG-MTOR-C1483F

    Plasmid

    #69008

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-C1483F (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Cysteine 1483 to Phenylalanine

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA3 Flag-humanTRAF3 (1096)

    Plasmid

    #66928

    Purpose

    Mammalian Expression of TRAF3

    Depositor

    Michael Karin

    Insert

    TRAF3 (TRAF3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-TDsmURFP-IRES-eGFP

    Plasmid

    #80344

    Purpose

    Expresses Tandem Dimer smURFP and eGFP in mammalian cells.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

    Plasmid

    #183252

    Purpose

    Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    anti-CD19 scFvFc (FMC-63)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-Flag-mRIPK1-S321E

    Plasmid

    #115340

    Purpose

    Mammalian expression of Flag-mRIPK1-S321E

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321E

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1/opto-a1AR-EYFP

    Plasmid

    #20947

    Purpose

    Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-a1AR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5FRT/Flag-LOC339287(MSL1)

    Plasmid

    #29762

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    MSL1 (MSL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only