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164,429 results

Showing: 5151 - 5200 of 164429 results

  1. GPRC5D-DuET

    Plasmid
    #213298
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPRC5D (GPRC5D Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213298-plasmid-map-sequence-id-430842

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  15. 139H2_HC

    Plasmid
    #206201
    Purpose
    For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.
    Depositor
    Joost Snijder
    Article
    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202
    Insert
    anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
    Use
    Tags
    -AAAHHHHHHHH
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    206201-plasmid-map-sequence-id-408741

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  18. pLenti.PGK.LifeAct-GFP.W

    Plasmid
    #51010
    Purpose
    Lentivector that expresses LifeAct-GFP from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-GFP
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51010-plasmid-map-sequence-id-291815

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  19. pUASTattb-cAMPr Y205A, R739A

    Plasmid
    #84933
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.
    Depositor
    Justin Blau
    Article
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R739A
    Use
    Tags
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R739A
    Promoter
    5X UAS
    Available Since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84933-plasmid-map-sequence-id-180303

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  21. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid
    #100050
    Purpose
    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    hChR2(E123T/H134R)
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized, E123T/H134R
    Promoter
    CaMKIIa
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100050-plasmid-map-sequence-id-194451

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  26. pAAV-hSyn-sbGLuc-B7-EYFP

    Plasmid
    #114110
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.
    Inserts
    Gaussia luciferase, slow burn
    B7 hinge region
    Enhanced Yellow Fluorescent Protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    114110-plasmid-map-sequence-id-252520

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  28. pYCTK034

    Plasmid
    #176738
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Saccharomyces cerevisiae (barrier part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    bar1Sc
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176738-plasmid-map-sequence-id-351575

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  29. hU6-Sa_tracr

    Plasmid
    #135973
    Purpose
    Contains tracrRNA-hU6 sequence for double sgRNA cloning
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135973-plasmid-map-sequence-id-313948

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  30. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On 3G Bidirectional
    Available Since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92099-plasmid-map-sequence-id-180428

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  36. cMET(1-960):cMET(1-960) BiBiT Vector

    Plasmid
    #238767
    Purpose
    Express cMET(1-960) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    cMET (MET Human)
    cMET (MET Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    (1-960)
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238767-plasmid-map-sequence-id-479905

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  37. Lenti NbALFA-mNeonGreen puro

    Plasmid
    #193973
    Purpose
    lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, puromycin resistance
    Depositor
    Raphael Gaudin
    Insert
    NbALFA-mNeonGreen
    Use
    Lentiviral
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    193973-plasmid-map-sequence-id-384356

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  41. PD-L1-HaloTag Fusion Vector

    Plasmid
    #237128
    Purpose
    Express PD-L1-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PD-L1 (CD274 Human)
    Use
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237128-plasmid-map-sequence-id-474958

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  43. pcDNA3-InPTapp-Kras

    Plasmid
    #235999
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    InPTapp-Kras (PLEKHA1 Human, Synthetic)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    235999-plasmid-map-sequence-id-473745

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  44. pcDNA3-InPGrp-Kras

    Plasmid
    #236001
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1
    Insert
    InPGrp-Kras (Cyth3 Rat, Synthetic)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    236001-plasmid-map-sequence-id-473749

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  46. pCAG-GluA2-Fab-HC-Halo

    Plasmid
    #230061
    Purpose
    Expresses heavy chain Fab fragment of anti-GluA2 fused to HaloTag. Can be used with light chain to produce Fab in HEK cells.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 Fab heavy chain fragment
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230061-plasmid-map-sequence-id-460113

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  47. TFORF0105

    Plasmid
    #142535
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TRERF1 (TRERF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    142535-plasmid-map-sequence-id-454980

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  49. pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid
    #221076
    Purpose
    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication
    Depositor
    Mikhail Shapiro
    Article
    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.
    Insert
    GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221076-plasmid-map-sequence-id-438459

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  50. pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid
    #221077
    Purpose
    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication
    Depositor
    Mikhail Shapiro
    Article
    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.
    Insert
    CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221077-plasmid-map-sequence-id-438460

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Showing: 5151 - 5200 of 164429 results