Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,160 results

Showing: 5151 - 5200 of 177160 results

  4. Ezh2[FL]-dCas9

    Plasmid
    #100086
    Purpose
    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    14056 (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[FL]-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    100086-plasmid-map-sequence-id-197550

    Enlarge

  12. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid
    #74707
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence
    Depositor
    Eugene Yeo
    Publication
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    Expression
    Mammalian
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    74707-plasmid-map-sequence-id-152737

    Enlarge

  13. pcDNA Flag Lamp2b-HA

    Plasmid
    #71292
    Purpose
    Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.
    Depositor
    Joshua Leonard
    Publication
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    Flag, HA, and Lamp2 signal peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71292-plasmid-map-sequence-id-131923

    Enlarge

  16. pSBtet-Hyg

    Plasmid
    #60508
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    60508-plasmid-map-sequence-id-97539

    Enlarge

  18. p201N Cas9

    Plasmid
    #59175
    Purpose
    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
    Depositor
    Wayne Parrott
    Publication
    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    nptII
    Use
    CRISPR
    Promoter
    2x35S and StUbi-3P
    Available since
    Sept. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    59175-plasmid-map-sequence-id-266922

    Enlarge

  20. mCherry-ARP2-N-14

    Plasmid
    #54980
    Purpose
    Localization: Actin Related Protein, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ARP2 (ACTR2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  24. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50463
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    50463-plasmid-map-sequence-id-75416

    Enlarge

  34. pTriEx-mVenus-PA-Rac1

    Plasmid
    #22007
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles

  37. tTA IRES GFP

    Plasmid
    #18783
    Depositor
    Scott Lowe
    Insert
    tTA
    Use
    Retroviral
    Tags
    IRES GFP
    Expression
    Mammalian
    Available since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles

  44. pLNCX chick v-src

    Plasmid
    #14578
    Depositor
    Joan Brugge
    Insert
    v-Src (SRC Chicken)
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    April 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles

  45. pcDNA3-T7-ERK1

    Plasmid
    #14440
    Depositor
    John Blenis
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERK1 (Mapk3 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Available since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    14440-plasmid-map-sequence-id-254030

    Enlarge

  49. pDest-544

    Plasmid
    #11519
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    His6-NusA
    Expression
    Bacterial
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    11519-plasmid-map-sequence-id-6969

    Enlarge

Showing: 5151 - 5200 of 177160 results