159,557 results
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pHDM-VSV-G
Plasmid#164440PurposeEncodes VSV-G protein for pseudovirus productionDepositorInsertVSV-G
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF3018
Plasmid#144494PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNEUROG3 (NEUROG3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-Furin
Plasmid#179920PurposeFurin proteinDepositorInsertFurin
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP1950
Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-PinkyCaMP
Plasmid#232856PurposeAn AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under hSyn promoterDepositorInsertPinkyCaMP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
p13xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180480PurposeInducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInsert13x QUAS-ZsGreen-P2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mCherry-EGFP-LC3B
Plasmid#170446PurposeLentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.DepositorInsertmCherry-GFP-LC3 (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHdzCas9-KRAB
Plasmid#92341PurposeExpression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistanceDepositorTypeEmpty backboneUseCRISPR; Plant expressionTagsKRABExpressionPlantMutationPromoterCaMV35SAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2
Plasmid#113886PurposeMammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2DepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2
Plasmid#217635PurposeAAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.DepositorInsertmU6-sgRNA, Handle sequence, EF1a-mTagBFP2
UseAAVTagsExpressionMutationPromoterEF1alpha and mU6Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDD103
Plasmid#119887PurposeConstitutive expression of mCherry on pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpConstitutive_mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee
Plasmid#140836Purposemammalian expression of SpiCee, a buffer of calcium that prevents downstream signalingDepositorInsertSpiCee
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-VARNAM2
Plasmid#195520PurposeRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoterDepositorInsertVARNAM2
UseAAVTagsExpressionMammalianMutationVARNAM SI linkerPromoterSynapsinAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2
Plasmid#202566PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopyDepositorInsertLINE-1
UseTagsmNeonGreen2 on ORF1pExpressionMammalianMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MCS-P2A-sfGFP
Plasmid#74668PurposePlasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.DepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-bjGFP
Plasmid#67032PurposePlasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertsB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
bjGFP
UseConjugative bacterial vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPS0379
Plasmid#115510PurposeSuitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFPDepositorInsertRlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)
Plasmid#197511PurposeCbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacerDepositorInsertAAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
UseAAV and CRISPRTagsBPNLS and BPNLS-NpuC(intein)ExpressionMutationC-terminal mutations of SpRY(L1111R/D1135L/S1136W…PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-sgRNA
Plasmid#177783PurposeOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegansDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBG‐AMPK α1(1‐312)
Plasmid#27632DepositorInsertAMPK α1(1‐312) (Prkaa1 Rat)
UseTagsGSTExpressionMammalianMutationConstitutively active by truncation after am…PromoterAvailable sinceMay 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-TEV
Plasmid#69929Purposebacterial expression of MBP-TEV n-terminal fusionsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsMBP and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2-myc-mCherry
Plasmid#86159PurposeFLS2-mCherry expressionDepositorInsertFLS2 (FLS2 Mustard Weed)
UseTagsmCherry and mycExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRL_GI_PCB28
Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ma-pREP
Plasmid#197571PurposePositive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a oriDepositorInsertsChloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
UseTagsExpressionBacterialMutation111TAG and 8TAG/112TAGPromoterAraC, T7, cat promoter (constitutive), and lpp (c…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n
Plasmid#47929Purposeexpression of an optimized Cas9 for genome-editing in zebrafishDepositorInsertnls-zcas9-nls
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
ampkar
Plasmid#35097PurposeA fluorescent reporter of AMPK activity and cellular energy stressDepositorInsertampkar
UseTagsECFP and VenusExpressionBacterialMutationnaPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
hNFAT2-409:TALE:VP64:KRɸ
Plasmid#160661PurposeConstitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.DepositorInserthNFAT2-409:TALE:VP64:KRɸ
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0381
Plasmid#133231PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ heiCas9
Plasmid#186929PurposeFor in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLSDepositorInsertmyc-flexible linker-oNLS-Cas9-oNLS
UseCRISPRTagsmyc-flexible linker-optimizedNLS and optimizedNLSExpressionMammalianMutationPromotersp6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAF0035 Ef1a-Nm60-2a-EGFP
Plasmid#193464PurposeExpression of Nm60 recombinase from Ef1a promoterDepositorInsertNm60
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLT3-ITD-MSCV-IRES-GFP
Plasmid#184779PurposeExpresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cellsDepositorInsertFLT3 (FLT3 Human)
UseRetroviralTagsExpressionMammalianMutationActivating internal tandem duplication (ITD) adde…PromoterMSCVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCM351
Plasmid#46013DepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoternaAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSII416
Plasmid#35456DepositorTypeEmpty backboneUseYeast centromere vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ULK2
Plasmid#97075PurposeExpresses ULK2 in mammalian cellsDepositorInsertULK2 (ULK2 Human)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP1
Plasmid#71465PurposeEncodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 1 (SENP1 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_BFP2_WPRE
Plasmid#91787PurposeConstitutive expression of mTagBFP2 using PGK promoter in a lentiviral backbone.DepositorInsertmTagBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)
Plasmid#188375PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex.mcs sv40promoter-luc
Plasmid#129429PurposeLuc expression under SV40 promoterDepositorInsertLUC
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2699
Plasmid#144349PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKMT2B (KMT2B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIX405-KANK1
Plasmid#121984Purposetetracycline-inducible expression of C-terminal GFP tagged KANK1DepositorInsertKANK1 (KANK1 Human)
UseLentiviralTagsEmerald GFPExpressionMutationPromoterTight TRE promoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-1
Plasmid#16400PurposeAdenoviral vector for use in AdEasy System.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET/ELP-I-CAT
Plasmid#71461PurposeELP-intein purification systemDepositorTypeEmpty backboneUseTagsELP ((VPGXG)110) and inteinExpressionBacterialMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMJA285= pSin-TCRab-CMVp-Puro
Plasmid#116875Purposestable TCR expression in human T cellsDepositorUseLentiviralTagsExpressionMammalianMutationdeletion of EF1-a promotorPromoterCMV PromotorAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-UbC-VP64-dCas9-VP64
Plasmid#232179PurposeLentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cellsDepositorInsertVP64-dCas9-VP64
UseLentiviralTagsExpressionMammalianMutationD10A, H840APromoterhUbCAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pLenti X2 Hygro DEST (w17-1)
Plasmid#17295Purpose3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, HygroDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mTurquoise2
Plasmid#113723PurposeExpresses the fluorescent protein mTurquoise2 in a lentiviral backboneDepositorInsertmTurquoise2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only
