159,557 results
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EF1a_NEUROG3_P2A_Hygro_Barcode
Plasmid#120468PurposeBarcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertNEUROG3 (NEUROG3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXRM15
Plasmid#63150PurposeMet15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDESTpK7WG2mito
Plasmid#158726PurposePlant mitoTALEN vector assembly, Destination vectorDepositorInsertmitochondrial presequence in front of attR1 in pk7WG2
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p35S::AtSOBIR1-mCherry
Plasmid#86166PurposeSOBIR1-mCherry expressionDepositorInsertSOBIR1 (SOBIR1 Mustard Weed)
UseTagsmCherryExpressionPlantMutationPromoter35SAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS/D614G
Plasmid#164566PurposeSARS-CoV-2 Spike protein with D614G mutation (S-GSAS-D614G variant)DepositorInsertSpike (S-GSAS-D614G variant)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP C1
Plasmid#202424PurposeEncodes GFP aloneDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta EC
Plasmid#202423PurposeExpression of GFP-tagged PODXL without extracellular domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationdeleted extracellular domainPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL 5N>Q
Plasmid#202419PurposeExpression of GFP-tagged PODXL with mutated N-linked glycosylation sitesDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationN33/43/104/144/360QPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL-SBP-EGFP-LAMP1
Plasmid#222317PurposeSynchronize the trafficking of LAMP1 from the ER.DepositorInsertStreptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2796
Plasmid#63641PurposepCfB2796 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoternoneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP22
Plasmid#22575DepositorInsertUSP22 (USP22 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAR17_StrepI_hFASh_H8_pET22b
Plasmid#122846PurposeExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInserthuman FASN/ human fatty acid synthase (FASN Human)
UseTagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 F-tractin-EGFP
Plasmid#58473PurposeExpresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoterDepositorInsertF-tractin (Itpka Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pREDCas9
Plasmid#71541PurposeConstitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
lambda red genes
plasmid curing system
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_CalR_HaloTag9_KDEL
Plasmid#175540PurposeExpression of HaloTag9 in the endoplasmid reticulum of mammalian cellsDepositorInsertCalR-HaloTag9-KDEL
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Paxillin-22
Plasmid#58024PurposeLocalization: Focal Adhesions, Excitation: 555, Emission: 584DepositorInsertPaxillin
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-FGFR3-S249C
Plasmid#116386PurposeLentiviral expression of FGFR3 S249CDepositorInsertFGFR3 (FGFR3 Human)
UseLentiviralTagsExpressionMutationS249CPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ULK4
Plasmid#23849DepositorInsertULK4 (ULK4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF0793
Plasmid#142692PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF5 (ATF5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLO99
Plasmid#90219PurposeComplements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)DepositorInsertATET_03691
UseAspergillusTagsExpressionMutationPromoterpLacAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCR8/GW/TOPO-TBX20a
Plasmid#98620PurposeGateway entry TOPO TA cloning vector for TBX20aDepositorInsertTbx20a (Tbx20 Mouse)
UseTopo ta cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-Hs4E-BP3
Plasmid#79439PurposeExpresses lambdaN-HA-tagged human 4E-BP3 in Mammalian cellsDepositorInsertHs4E-BP3 (EIF4EBP3 Human)
UseTagslambdaN-HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-TRE>{MCS}:T2A:EGFP
Plasmid#236250PurposeHygromycin selected lentiviral vector with MCS for tetracycline-inducible gene, for use with dual-inducible CymR-CuO systemDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA HA DUSP6
Plasmid#236430Purposetransient overexpression of DUSP6 in mammalian cellsDepositorInsertDUSP6 (DUSP6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-ExRai-CKAR2
Plasmid#236098PurposePlasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.DepositorInsertLyn-ExRai-CKAR2
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
plenti-ITGB6-HA
Plasmid#235242PurposeLentiviral vector to generate HA-tagged human ITGB6 stable expressing cell lineDepositorInsertITGB6 (ITGB6 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CAG-FLEX-MCS-WPRE-SV40 - GCH2
Plasmid#230815PurposeAAV cloning vector for CRE dependent expression under the CAG promoter.DepositorTypeEmpty backboneUseAAV and Cre/Lox; Cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26
Plasmid#232929PurposeTargeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.DepositorInsertOsTir1(F74G)
UseMouse TargetingTagsExpressionMammalianMutationchanged Phenylalanine 74 to GlycinePromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-PspCas13b-NES-mCherry
Plasmid#233608PurposeExpression of PspCas13b in mammalian cellsDepositorInsertPspCas13b-NES-mCherry
UseCRISPRTags3xFlag and mCherryExpressionMutationPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC016-gPsp-EGFP_start_codon
Plasmid#233613PurposeExpression of guide RNA targeting the start codon of the EGFP gene for PspCas13bDepositorInsertgRNA targeting EGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)
Plasmid#221329PurposeDox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transductionDepositorUseRetroviralTagsExpressionMutationG12VPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR Ef1a-dCas9-BFP-KRAB
Plasmid#217304Purposelentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoterDepositorInsertdCas9-tagBFP-KRAB
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMutationPromoterEf1alphaAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-Mch-MAP1LC3B
Plasmid#201924PurposeFor lentiviral transduction of Mch-MAP1LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pan-QKI scFv [N147/6]
Plasmid#211605PurposeMammalian Expression Plasmid of Pan-QKI scFv. Derived from hybridoma N147/6.DepositorInsertPan-QKI (Homo sapiens) recombinant mouse scFv. (QKI Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP10D(E215Q)-HA
Plasmid#209248PurposeMammalian expression of ATP10D mutant E215QDepositorInsertATP10D (ATP10D Human)
UseTagsHAExpressionMammalianMutationE215QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta IC
Plasmid#202422PurposeExpression of GFP-tagged PODXL without intracellular domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationdeleted intracellular domainPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta PBM + Ezrin
Plasmid#202418PurposeExpression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRINDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsEzrin and GFPExpressionMutationdelete PDZ binding motifPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRA1EGFP
Plasmid#193791PurposeExpresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPR promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NOTCH2(Trunc1)-COMP5AP-AviTag-9xHis
Plasmid#157611PurposeMammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertNOTCH2 (NOTCH2 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA7A-COMP5AP-AviTag-9xHis
Plasmid#157486PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSEMA7A (SEMA7A Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-JAG2-Fc(DAPA)-AviTag-6xHis
Plasmid#157027PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertJAG2 (JAG2 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Aldh1L1 scFv [N103/31]
Plasmid#190522PurposeMammalian Expression of Aldh1L1 scFV. Derived from hybridoma N103/31.DepositorInsertAldh1L1 (Rattus norvegicus) recombinant scFV (Aldh1l1 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5xQUAS-ZsGreen-P2A, α-cry:mCherry
Plasmid#180477PurposeInducible gene expression vector p5xQUAS-ZsGreen-P2A, α-cry:mCherryDepositorInserts5x QUAS-ZsGreen-P2A
mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H170
Plasmid#170352PurposemTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseAdenoviralTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only
