19,771 results
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PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT306 Kp03 Lenti pEF-attB LSR GFP
Plasmid#184949PurposeLentiviral delivery of recombinase landing padDepositorInsertKp03 Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAX APLP1
Plasmid#30141DepositorInsertAmyloid Precursor Like Protein 1 (APLP1 Human)
ExpressionMammalianAvailable SinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET iGluSnFR3 v857
Plasmid#175186PurposeFluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.DepositorInsertiGluSnFR3 v857
UseSynthetic BiologyTagsHis tag, T7 leader, Xpress epitopeExpressionBacterialPromoterT7Available SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a
Plasmid#84476PurposeLentiviral construct to express dCas9 fused with Dnmt3aDepositorAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD1-dU6:1gRNA
Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFOK
Plasmid#166651PurposeSuicide vector for gene manipulationDepositorInsertI-sceI, sacB
UseSynthetic BiologyAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Bx
Plasmid#51552PurposeExpresses Bxb1 integrase in mammalian cells.DepositorInsertBxb1 integrase
ExpressionMammalianPromoterCMVAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRN110
Plasmid#84462PurposepJB38-NWMN29-30 + SarA_P1-mKate2-TermDepositorInsertSarA_P1-mKate2 from pRN11
Available SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TyrRS-His
Plasmid#124121PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFE-Puro
Plasmid#170069PurposeExpresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoterDepositorInsertCas9 guide RNA scaffold with the F+E scaffold modification
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcrDNA3.1-PRKACA
Plasmid#100890PurposeMammalian expression of PRKACADepositorInsertProtein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
ExpressionMammalianMutationIsoform 1 wild typePromoterCMVAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEv-Fat4-citrine
Plasmid#112854PurposeConstitutive expression of human fat4 protein fused to citrine fluorescent proteinDepositorAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
1481 pcDNA3 flag HA 14-3-3 zeta
Plasmid#9002DepositorAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDusk
Plasmid#43795PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_GFP
Plasmid#96941PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertGreen fluorescent protein
UseLentiviralPromoterCaMKIIaAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-RIPK1
Plasmid#78846PurposeTo detect localization of RIPK1 protein in live mammalian cellsDepositorAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOpen-MMLV_RT (lack H)
Plasmid#165556PurposeSingle 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.DepositorInsertMoloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
UseSynthetic BiologyAvailable SinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHSB04X
Plasmid#117258PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertCas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
UseOtherAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70
Plasmid#15215DepositorAvailable SinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx3
Plasmid#116855PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
TagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamino acids 169-329 (tandem trimer)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK7-HA-S6K1-WT
Plasmid#8984DepositorAvailable SinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight Blast DEST (w762-1)
Plasmid#26434DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorExpressionMammalianAvailable SinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
p38-KTR-mStrawberry reporter
Plasmid#158687PurposeThis plasmid is a p38 pathway reporter. It has CMV promoter driving the expression of p38-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mGreenLantern
Plasmid#164459PurposeLocalization of mGreenLantern fluorescent protein to actinDepositorInsertmGreenLantern
TagsLifeActExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR
Plasmid#112159PurposeGABA SensorDepositorHas ServiceAAV1InsertiGABASnFR
UseAAVPromoterhSynapsinAvailable SinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-ValRS-His
Plasmid#124122PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-V5-Topo mCherry
Plasmid#128062PurposeLentiviral vector for C terminal mCherry fusionsDepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIRT4 Flag
Plasmid#13815PurposeMammalian expression of human SIRT4 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR7-YFP
Plasmid#13022DepositorAvailable SinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI WT
Plasmid#167289PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-IDepositorAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS-kI
Plasmid#51553PurposeMammalian phiC31 integrase expression vector. For use in applications where pseudosite integration is not desired.DepositorInsertphiC31 integrase
ExpressionMammalianAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Flag-cIAP2/pRK5
Plasmid#27973DepositorAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rtTA
Plasmid#175274PurposeAAV vector mediating neuron-specific expression of rtTADepositorInsertReverse tetracycline-controlled transactivator (rtTA)
UseAAVPromoterSynapsinAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-1
Plasmid#208790PurposeInducibly expresses human Caspase1, fused to light-activatable Cry2DepositorAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
hGli1 flag3x
Plasmid#84922Purposemammalian expression of Gli1DepositorAvailable SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet
Plasmid#104006PurposePlasmid encoding mScarletDepositorInsertmScarlet
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXspuro-GFP-NBR1
Plasmid#74202PurposeRetroviral expression of GFP-NBR1DepositorAvailable SinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE
Plasmid#130999PurposeDouble floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoterDepositorInsertChRmine
UseAAVTagsmScarlet-Kv2.1PromoterEf1aAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
hu-TLR4 cDNA wt pDEST40
Plasmid#42646Purposedestination vector for expression of human TLR4 in mammalian cellsDepositorAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330S-3
Plasmid#58779PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF278
Plasmid#167673PurposeIntermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PJ-INPP5E
Plasmid#38001DepositorTagsmRFPExpressionMammalianMutationCys 779 mutated to SerAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
TagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianPromoterSFFVAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mScarletI
Plasmid#137801PurposeVisualization of microtubulesDepositorInsertEnsconsin microtubule binding domain
ExpressionMammalianPromoterCMVAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB2076 pAAV REPAIR.t1
Plasmid#176323PurposepAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)DepositorInsertshuman codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site
UseAAV and CRISPRTags3xHA and HIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterEFS (short EF1alpha) and hU6Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-HEK3_CTT_ins
Plasmid#132778PurposeS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editingDepositorInsertHEK3_CTT_ins pegRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only
