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We narrowed to 18,512 results for: tec

Showing: 5351 - 5400 of 18512 results

  1. pJRH-1362 scFv entry plasmid

    Plasmid
    #201912
    Purpose
    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Insert
    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201912-plasmid-map-sequence-id-403308

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  4. pRDA_722

    Plasmid
    #216077
    Purpose
    CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral; Destination
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216077-plasmid-map-sequence-id-431423

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  6. pOCC305

    Plasmid
    #118898
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and TwinStrep-MB…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118898-plasmid-map-sequence-id-232364

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  14. U6-5'+3' MS2-CircRNA

    Plasmid
    #213752
    Purpose
    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Insert
    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213752-plasmid-map-sequence-id-422189

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  15. pR26 CAG/GFP Asc

    Plasmid
    #74285
    Purpose
    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI
    Depositor
    Ralf Kuehn
    Article
    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi
    Insert
    Rosa26 5-homology region
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    74285-plasmid-map-sequence-id-357226

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  16. pAGK61

    Plasmid
    #228121
    Purpose
    Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228121-plasmid-map-sequence-id-455410

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  17. pAGK62

    Plasmid
    #228122
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228122-plasmid-map-sequence-id-455403

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  20. PX551

    Plasmid
    #60957
    Purpose
    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    pMecp2
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60957-plasmid-map-sequence-id-181490

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  25. Fragment1 with TLS2

    Plasmid
    #196983
    Purpose
    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Intermediate fragment for assembly of gRNA-TLS2 construct
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196983-plasmid-map-sequence-id-390457

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  33. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  42. pR26 CAG/GFP Dest

    Plasmid
    #74281
    Purpose
    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning
    Depositor
    Ralf Kuehn
    Article
    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi
    Insert
    Rosa26 5-homology region
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    74281-plasmid-map-sequence-id-419887

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  47. DR274

    Plasmid
    #42250
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription
    Depositor
    Keith Joung
    Article
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR; Zebrafish expression
    Tags
    Expression
    Worm
    Mutation
    Promoter
    T7
    Available since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    42250-plasmid-map-sequence-id-254852

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  50. pGTMV

    Plasmid
    #118755
    Purpose
    Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.
    Depositor
    Jose-Antonio Daros
    Article
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    118755-plasmid-map-sequence-id-229554

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Showing: 5351 - 5400 of 18512 results