166,169 results

1. pAAV-Ef1a-mCherry-IRES-Cre

   Plasmid

   #55632

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Inserts

   mCherry
   Cre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a and Ef1a/IRES

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330.puro

   Plasmid

   #110403

   Purpose

   CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

   Depositor

   Sandra Martha Gomes Dias

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIN-VS1

   Plasmid

   #197783

   Purpose

   Level 2 destination vector: pMIN-VS1 backbone

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 puro_shTBK1_3185

   Plasmid

   #167294

   Purpose

   Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   shRNA TBK1 (TBK1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIPZ-shNTC

   Plasmid

   #217638

   Purpose

   Dox inducible shRNA non targeting control shRNA

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   shNTC
   

   Use

   RNAi

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. hKDR

   Plasmid

   #83436

   Purpose

   human VEGFR2 with V5 and tGFP tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

   Insert

   human VEGFR2 (KDR Human)
   

   Tags

   V5 and turboGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP14467 - pAAV-AiE2182m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4467)

   Plasmid

   #224043

   Purpose

   AiE2182m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_IT neurons (strong)

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6_empty-sgRNA(PP7)

   Plasmid

   #232432

   Purpose

   empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged sgRNA scaffold driven by human U6 promoter
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKB01_mCherry

   Plasmid

   #69104

   Purpose

   Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH86966::AtU6p::sgRNA_PDS

    Plasmid

    #46966

    Purpose

    Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    AtU6p::sgRNA_PDS
    

    Use

    CRISPR; Plant expression

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUbC-nls-ha-stdMCP-stdGFP

    Plasmid

    #98916

    Purpose

    synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

    Depositor

    Robert Singer

    Article

    Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

    Insert

    stdMCP-stdGFP
    

    Use

    Lentiviral

    Tags

    GFP

    Promoter

    UbC

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLDLR-Luc mutSRE

    Plasmid

    #14945

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    LDLR promoter mut SRE (LDLR Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Point mutation in the SRE-1 sequence of the LDL r…

    Available Since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMZ828

    Plasmid

    #155322

    Purpose

    Constitutive expression of PhyB(1-650)-VP16 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pROF021

    Plasmid

    #155326

    Purpose

    Expression of PULSE-controlled FLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-FLuc-TSV40
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE-tdTomato

    Plasmid

    #50798

    Purpose

    Expression of tdTomato under the control of the TRE-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    TREtight driven tdTomato fluorescent protein
    

    Use

    Mutli-species

    Promoter

    TREtight

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-HSPA8

    Plasmid

    #121161

    Purpose

    Mammalian expression of GFP tagged HSPA8

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    HSPA8 (HSPA8 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5-CBFbeta

    Plasmid

    #12427

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    CBF beta (Cbfb Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pKM461

    Plasmid

    #108320

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    SacRB
    

    Expression

    Bacterial

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMVie-NcanGFP

    Plasmid

    #235983

    Purpose

    Expresses the hyaluronan-binding domain of Neurocan fused to eGFP under the CMV promoter. It contains a sequence for secretory pathway, so the probe is exported to the extracellular space

    Depositor

    Federico Soria

    Article

    Fernández-Ballester et al bioRxiv 2025.01.31.635882; doi: 10.1101/

    Insert

    Neurocan-GFP
    

    Use

    AAV

    Tags

    GFP

    Promoter

    CMVie

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.2_TagBFP

    Plasmid

    #113724

    Purpose

    Expresses the fluorescent protein TagBFP in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. FLAG-3C-sGFP11_pcDNA3.1(-)

    Plasmid

    #182932

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Tags

    3C-cleavable split GFP 11, FLAG tag, and HA signa…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. TRUPATH Triple Ga13

    Plasmid

    #196062

    Purpose

    Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha13-RLuc8 (GNA13 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. hCathepsin B

    Plasmid

    #11249

    Purpose

    Mammalian expression of human cathepsin B

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Insert

    cathepsin B (CTSB Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Expression

    Bacterial

    Mutation

    E32K

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHAGE-CMV-Flag-CDK12_IDG-K

    Plasmid

    #135281

    Purpose

    Gateway destination clone of CDK12 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-CDK12 (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEGFP-C1-ER alpha

    Plasmid

    #28230

    Purpose

    Mammalian expression of Estrogen Receptor alpha fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

    Insert

    Estrogen Receptor alpha (ESR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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27. CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

    Plasmid

    #138418

    Purpose

    CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BioTAP-tag

    Promoter

    EF1a

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-GINKO1

    Plasmid

    #113112

    Purpose

    Mammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pVITRO1-mCardinal-CAXX

    Plasmid

    #184451

    Purpose

    Plasma membrane-targeted mCardinal expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    mCardinal-CAAX
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV-CAG-NLS-GFP

    Plasmid

    #104061

    Purpose

    AAV expression of nuclear localized GFP with SV40 NLS

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.eB

    Insert

    GFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GFP-RNF2

    Plasmid

    #126544

    Purpose

    Expresses GFP-tagged RNF2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RING1B (RNF2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMXs GFP-LC3-RFP

    Plasmid

    #117413

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

    Plasmid

    #218868

    Purpose

    Mammalian expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. BM00369

    Plasmid

    #155338

    Purpose

    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBABE-puro SV40 LT

    Plasmid

    #13970

    Depositor

    Thomas Roberts

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    SV40 LT (SV40gp6 )
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET GFP LIC cloning vector (2GFP-T)

    Plasmid

    #29716

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, SiriusGFP, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Tcf/Lef:H2B-GFP

    Plasmid

    #32610

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Ferrer-Vaquer et al BMC Dev Biol. 2010 Dec 21;10:121.

    Insert

    Tcf/Lef:H2B-GFP
    

    Promoter

    hsp68

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA3.1 hSCN5A

    Plasmid

    #145374

    Purpose

    expresses human Nav1.5 (WT) in mammalian cells

    Depositor

    Hugues Abriel, Robert Kass

    Article

    Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHS_Nova

    Plasmid

    #239831

    Purpose

    NovaIscB mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    NovaIscB
    

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Insertion of REC domain from NbaCas9-1 and swap o…

    Promoter

    CMV

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge