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174,739 results

Showing: 4521 - 4560 of 174739 results

  2. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

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  10. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Article
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135955-plasmid-map-sequence-id-269512

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  14. PX458-AAVS1

    Plasmid
    #113194
    Purpose
    Encodes gRNA for human AAVS1
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C, AAVS1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113194-plasmid-map-sequence-id-216314

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  21. pTP1174

    Plasmid
    #104162
    Purpose
    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104162-plasmid-map-sequence-id-200079

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  23. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Expression
    Mammalian
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    88892-plasmid-map-sequence-id-451853

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  32. pFUGW-hGtACR2-EYFP

    Plasmid
    #67877
    Purpose
    Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone
    Depositor
    John Spudich
    Article
    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.
    Insert
    hGtACR2
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    UbC
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67877-plasmid-map-sequence-id-199004

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  34. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available Since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62888-plasmid-map-sequence-id-105911

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  38. mNeptune2-N1

    Plasmid
    #54837
    Purpose
    Localization: N1 Cloning Vector, Excitation: 600, Emission: 650
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mNeptune2
    Expression
    Mammalian
    Available Since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54837-plasmid-map-sequence-id-162708

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  39. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50455
    Purpose
    Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50455-plasmid-map-sequence-id-214301

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Showing: 4521 - 4560 of 174739 results