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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 11,710 results for: pcDNA

Showing: 5951 - 6000 of 11710 results

  4. 827 pcDNA3 GFP PTEN 1-274

    Plasmid
    #10926
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Amino acids 1-274
    Promoter
    Available since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  8. pcDNA5/FRT/TO/Flag-tGFP-Cryz

    Plasmid
    #110378
    Purpose
    Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    crystallin zeta (CRYZ Human)
    Use
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110378-plasmid-map-sequence-id-213403

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  10. CFP(1-158)-gamma-1 in pcDNAI/Amp

    Plasmid
    #55615
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP(1-158)-gamma-1 (GNGT1 Bovine, Aequorea victoria)
    Use
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-1 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55615-plasmid-map-sequence-id-115069

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  11. CFP(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid
    #55616
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP (1-158)-gamma-2 (Gng2 Aequorea victoria, Mouse)
    Use
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-2 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55616-plasmid-map-sequence-id-115112

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  12. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid
    #112712
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    EGFP
    Use
    Tags
    HIV Rev nuclear localization signal (NES), biotin…
    Expression
    Mammalian
    Mutation
    Nucleotide sequence optimized for synthetic gene …
    Promoter
    CMV with Tet repressor binding sites
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    112712-plasmid-map-sequence-id-217058

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  36. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Tags
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  37. pcDNA5-FRT/TO-APT2-LAMA-G97

    Plasmid
    #234426
    Purpose
    Express APT2 with a C terminal fusion to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97
    Depositor
    William Fuller
    Article
    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:
    Insert
    APT2 fused to the GFP enhancer nanobody containing dihydrofolate reductase inserted in the nanobody at position G97 (LYPLA2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234426-plasmid-map-sequence-id-470093

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  38. PME-1 S156A (inakt.mut) pcDNA-StrepIII

    Plasmid
    #119293
    Purpose
    Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the methylesterase active center
    Depositor
    Jukka Westermarck
    Insert
    PPME1 (PPME1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S156A
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    119293-plasmid-map-sequence-id-232194

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  42. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

    Plasmid
    #233064
    Purpose
    Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233064-plasmid-map-sequence-id-464574

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  43. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

    Plasmid
    #233063
    Purpose
    Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Threonine 923 to Glycine
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233063-plasmid-map-sequence-id-464572

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Showing: 5951 - 6000 of 11710 results