We narrowed to 5,868 results for: nls

1. pET-His6-en15dFnCas9GFP

   Plasmid

   #201951

   Purpose

   Expression of en15dFnCas9-GFP in bacterial cells

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Expression of en15dFnCas9-GFP in bacterial cells
   

   Use

   CRISPR

   Tags

   6x His, HA, NLS, and mEGFP

   Expression

   Bacterial

   Mutation

   E1603H on FnCas9

   Promoter

   T7

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Anti-Neuroligin-4* [N98/47R]

   Plasmid

   #188168

   Purpose

   Mammalian Expression Plasmid of anti-Neuroligin-4* (Mouse). Derived from hybridoma N98/47

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Neuroligin-4* (Mus musculus) recombinant mouse monoclonal antibody (Nlgn4l Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAB(EXPR-DS-HDAC5_HDAC3)

   Plasmid

   #114399

   Purpose

   For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5_HDAC3 catalytic domain swap (HDAC3, HDAC5 Human)
   

   Use

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ288 (BoCas12a)

   Plasmid

   #138125

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BoCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   BoCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ282 (TsCas12a)

   Plasmid

   #138114

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized TsCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   TsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ283 (MlCas12a)

   Plasmid

   #138115

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MlCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   MlCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ285 (Lb5Cas12a)

   Plasmid

   #138120

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   Lb5Cas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ286 (CMaCas12a)

   Plasmid

   #138122

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized CMaCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   CMaCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPQ287 (BsCas12a)

   Plasmid

   #138123

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   BsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

    Plasmid

    #136657

    Purpose

    Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

    Plasmid

    #136654

    Purpose

    Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:CNRZ1066 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEMS1408

    Plasmid

    #29205

    Purpose

    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple201 (SLC6A5 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEMS1499

    Plasmid

    #29247

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple28 (CCL27 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pEMS1498

    Plasmid

    #29246

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple27 (CCL27 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pEMS1486

    Plasmid

    #29237

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple16 (C8orf46 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pEMS1500

    Plasmid

    #29248

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple29 (CCL27 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEMS1710

    Plasmid

    #29296

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple238 (TRH Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pYTK002

    Plasmid

    #65109

    Purpose

    Encodes ConLS as a Type 1 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYTK008

    Plasmid

    #65115

    Purpose

    Encodes ConLS' as a Type 1 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConLS'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. iF53 PB-Zim3-dCas9-mScarlet-Hygro

    Plasmid

    #204726

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. iF58 PB-Zim3-dCas9-mScarlet-Puro

    Plasmid

    #204728

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGC76

    Plasmid

    #19641

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <4xNLS-GFP::let-858(3’)
    

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGC78

    Plasmid

    #19654

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGC75

    Plasmid

    #19640

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <1xNLS-GFP::let-858(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGC79

    Plasmid

    #19655

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid

    #115344

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

    Depositor

    Steven Jacobsen

    Insert

    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEG302-SunTagng-22aa

    Plasmid

    #115345

    Purpose

    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome

    Depositor

    Steven Jacobsen

    Insert

    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGC257

    Plasmid

    #19660

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGGC012

    Plasmid

    #48826

    Purpose

    Provides GFP:NLS as GreenGate coding sequence module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    GFP:NLS
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGC302

    Plasmid

    #19668

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <*iA-4xNLS-mCherry::let-858(3’)
    

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGC310

    Plasmid

    #19669

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <*iA-1xNLS-mCherry::let-858(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGC307

    Plasmid

    #19662

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <*iA-1xNLS-CFP::let-858(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGC256

    Plasmid

    #19656

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGC305

    Plasmid

    #19646

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <*iA-1xNLS-GFP::let-858(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGC114

    Plasmid

    #19622

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGC231

    Plasmid

    #19644

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGC306

    Plasmid

    #19658

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <*iA-1xNLS-DsRed2::let-858(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGC293

    Plasmid

    #19657

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <*iA-4xNLS-DsRed2::let-858(3’)
    

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. L1P56

    Plasmid

    #195935

    Purpose

    Level 1 PhiC31 cassette with constitutive promoter : p35S:UbGln:PhiC31-NLS:tUBQ1

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    p35S:UbGln:PhiC31-NLS:tUBQ1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGC295

    Plasmid

    #19665

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <*iA-4xNLS-YFP::let-858(3’)
    

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge