164,577 results

1. pMOS008: GCaMP6F calcium sensor (cytosolic)

   Plasmid

   #163045

   Purpose

   GCaMP6F calcium sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   GCaMP6F
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-EF1aH N90-beta-catenin

   Plasmid

   #86499

   Purpose

   Mammalian expression of Myc tagged N90 beta-catenin

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-90; constitutively acti…

   Promoter

   EF1a

   Available Since

   Feb. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-CMV-EGFP-Hygro

   Plasmid

   #184593

   Purpose

   EGFP

   Depositor

   Robert Sobol

   Article

   Koczor et al Methods Mol Biol. 2023;2609:43-59. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-mRuby2

   Plasmid

   #40260

   Purpose

   RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Insert

   mRuby2
   

   Use

   Tags

   His6 and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Cas9-NLS-6xHis

   Plasmid

   #62933

   Purpose

   Expression of Cas9-NLS-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9-NLS-6xHis
   

   Use

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Ftractin-mCherry

   Plasmid

   #85131

   Purpose

   lentiviral expression of an F-actin live-cell marker (mCherry)

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PGKp-GFP-YAP (5SA)

   Plasmid

   #174174

   Purpose

   Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Five LATS phosphorylation sites (S61, S109, S127,…

   Promoter

   PGK

   Available Since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. ALIX-mNeonGreen

   Plasmid

   #191191

   Purpose

   Full-length ALIX with C-terminal mNeonGreen tag.

   Depositor

   Jin Zhang

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX (PDCD6IP Human, Synthetic)
   

   Use

   Tags

   6xHIS and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-ChrimsonR-GFP

   Plasmid

   #58806

   Purpose

   AAV expression of ChrimsonR-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K176R

   Promoter

   Syn

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINE GFP nanobody

    Plasmid

    #49172

    Purpose

    Bacterial expression of GFP nano body

    Depositor

    Brett Collins

    Article

    Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. d

    Insert

    GFP nanobody
    

    Use

    Tags

    HHHHHH

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW63.YY1.FKBP.knock-in.BFP

    Plasmid

    #104371

    Purpose

    For knocking in FKBP F36V P2A BFP

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    FKBP12(F36V) -P2A-BFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZNRF3-SmBiT, BiBiT-Ready Vector

    Plasmid

    #237083

    Purpose

    Express ZNRF3-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ZNRF3 (ZNRF3 Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pTP1172

    Plasmid

    #104161

    Purpose

    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 3xHA-KillerRed-OMP25

    Plasmid

    #174544

    Purpose

    localizes KillerRed to mitochondria

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    3xHA-KillerRed-OMP25
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458-mcherry

    Plasmid

    #161974

    Purpose

    Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    Cas9-t2a-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1-FUS/TLS-FLAGC

    Plasmid

    #60362

    Purpose

    Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDZ2087

    Plasmid

    #92414

    Purpose

    Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

    Insert

    TEV protease
    

    Use

    Tags

    MBP (self cleaving)

    Expression

    Bacterial

    Mutation

    L56V/S135G/S219V

    Promoter

    tac

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM8031

    Plasmid

    #48037

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211462

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKmK.P2

    Plasmid

    #125035

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    PDC1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

    Plasmid

    #90332

    Purpose

    CRISPR interference

    Depositor

    Dirk Heckl

    Article

    Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

    Inserts

    dCas9
    na
    SFFV
    P2A-BSD
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQE-80L-V4-ELP-EEP13

    Plasmid

    #237223

    Purpose

    Expresses micelle-forming elastin-like polypeptide (ELP)-endosomal escape peptide (EEP) fusion construct in bacteria

    Depositor

    Elliot Chaikof

    Article

    Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

    Insert

    V4-ELP-EEP13
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-HA-ER Y537S

    Plasmid

    #49499

    Purpose

    Expresses HA-tagged ER Y537S mutant in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tyrosine 537 to Serine

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-MAPPER

    Plasmid

    #117721

    Purpose

    MAPPER Selectively Labels ER-PM Junctions

    Depositor

    Jen Liou

    Article

    Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

    Insert

    MAPPER
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAWP78-PVCpnf1-16

    Plasmid

    #198271

    Purpose

    Unmodified pPVC; contains WT PVC structural/accessory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf1-16
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLp_3050sNuc

    Plasmid

    #122030

    Purpose

    Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

    Depositor

    Geir Mathiesen, Vincent Eijsink

    Article

    Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

    Insert

    signal peptide Lp_3050
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    sppA

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKmK.T4

    Plasmid

    #125056

    Purpose

    Storage plasmid for K.marxianus terminator; type 4 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    PDC1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mKikGR-N1

    Plasmid

    #54756

    Purpose

    Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mKikGR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

29. pAAV-FLEX-EGFP-KASH

    Plasmid

    #154373

    Purpose

    Vector for Cre-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKmK.P7

    Plasmid

    #125040

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    TEF1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV BiP-Myc-KDEL-wt

    Plasmid

    #27164

    Purpose

    Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Use

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pV1093

    Plasmid

    #111428

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST

    Plasmid

    #174007

    Purpose

    Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Has Service

    AAV9

    Insert

    jGCaMP8s-P2A-ChrimsonR-ST
    

    Use

    AAV

    Tags

    6xHis and soma targeting motif

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEGFP_TRPM8

    Plasmid

    #64879

    Purpose

    Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

    Insert

    Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

    Plasmid

    #188375

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBZS-M.CviPI

    Plasmid

    #198355

    Purpose

    Express M.CviPI in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    M.CviPI (M.CviPI )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pICE-HA-NLS-I-PpoI

    Plasmid

    #46963

    Purpose

    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    NLS-I-PpoI
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TET

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMIEG3-c-Jun

    Plasmid

    #40348

    Depositor

    Alexander Dent

    Article

    Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

    Insert

    c-Jun (JUN Human)
    

    Use

    Retroviral

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge