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173,051 results

Showing: 4961 - 5000 of 173051 results

  2. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  4. IDG_SCN2B_OE_1

    Plasmid
    #161624
    Purpose
    Overexpresses SCN2B
    Depositor
    Mike McManus
    Insert
    SCN2B (SCN2B Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    161624-plasmid-map-sequence-id-334519

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  10. dCas9-GCN5 FL

    Plasmid
    #179552
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179552-plasmid-map-sequence-id-354751

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  23. TFORF0528

    Plasmid
    #142643
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PAX6 (PAX6 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    142643-plasmid-map-sequence-id-427304

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  26. mCerulean-Rab7a-7

    Plasmid
    #55389
    Purpose
    Localization: Ras GTPase, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Rab7a (RAB7A Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    55389-plasmid-map-sequence-id-457779

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  36. pAAV asyn S129D

    Plasmid
    #36069
    Depositor
    Patrick Aebischer, Hilal Lashuel
    Insert
    alpha-synuclein S129D (SNCA Human)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    S129D
    Promoter
    CMV
    Available Since
    Sept. 10, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 4961 - 5000 of 173051 results