161,398 results

1. HO-hisG-URA3-hisG-poly-HO

   Plasmid

   #51663

   Purpose

   yeast plasmid for integration of target sequence at HO locus, URA3 selection

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF3198

   Plasmid

   #144674

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   OVOL2 (OVOL2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV Blast DEST (706-1)

   Plasmid

   #17451

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2/7

   Plasmid

   #112863

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap7
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. NET33 pEGFP-N2 (597)

   Plasmid

   #61989

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET33 (SCARA5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_078

   Plasmid

   #158582

   Purpose

   Lentiviral expression of BE4max and customizable gRNA

   Depositor

   John Doench, David Root

   Article

   Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEL066

   Plasmid

   #124897

   Purpose

   For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor

   Depositor

   Yong Zhang

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-GCN5 FL

   Plasmid

   #179552

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CRX-T2A-NeuroD1

   Plasmid

   #194607

   Purpose

   Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

   Depositor

   Haruhisa Inoue

   Article

   Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

   Insert

   CRX-T2A-NeuroD1
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH6HTC_CCT3-Halo

    Plasmid

    #175322

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCT3 (CCT3 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/Puro-CAG-JEDI-1P

    Plasmid

    #202607

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. HTR1F-Tango

    Plasmid

    #66408

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR1F (HTR1F Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. HA GSK3 beta S9A pcDNA3

    Plasmid

    #14754

    Purpose

    Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

    Depositor

    Jim Woodgett

    Article

    Stambolic et al Biochem J. 1994 Nov 1. 303 ( Pt 3)():701

    Insert

    GSK3 beta S9A (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A. Constitutively active mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pKAR2-Br512

    Plasmid

    #161875

    Purpose

    Expresses Br512 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Maranhao et al medRxiv

    Insert

    Modified DNA Polymerase I Large Fragment (Br512)
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Mutation

    Promoter

    T7, tetPA

    Available since

    Nov. 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV-DIO-EF1α-mKate2-biPAC

    Plasmid

    #169127

    Purpose

    AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-biPAC
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    codon optimized to mouse

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA4/TO-GFP-SPOP (WT)

    Plasmid

    #197008

    Purpose

    Mammalian inducible expression vector for GFP-tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    -

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHen2 Avi-tag-linker-p3

    Plasmid

    #119819

    Purpose

    For cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    linker with Avi-tag and p3
    

    Use

    Tags

    Avi-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-scFv-GCN4-mStayGold-GB1-dWPRE

    Plasmid

    #231598

    Purpose

    Express scFv_GCN4-mStayGold for SunTag translation imaging

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    scFv-GCN4-mStayGold
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. PRDX1A-c001

    Plasmid

    #175473

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX1 (PRDX1 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.hSyn.flex.H2B.GFP

    Plasmid

    #170368

    Purpose

    Cre-inducible AAV vector expressing nuclear GFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    flex H2B GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pD649-HAsp-TNFRSF12A-COMP5AP-AviTag-9xHis

    Plasmid

    #157052

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TNFRSF12A (TNFRSF12A Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. PhyB-mCherry-PEX3

    Plasmid

    #51572

    Purpose

    Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    PEX3 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

    Plasmid

    #202606

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P-P2A-mCherry-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAL119-TK

    Plasmid

    #21911

    Depositor

    Maria Castro

    Article

    Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

    Insert

    HSV Type 1 thymidine kinase
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT3TS-spCas9 ABE-Umax-ex2-rest1

    Plasmid

    #222143

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT3TS-spCas9 ABE-Umax-ex1-rest1

    Plasmid

    #222140

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HBV 1.3-mer X-null replicon

    Plasmid

    #65461

    Purpose

    containing 1.3 units of the X-null HBV genome (subtype ayw)

    Depositor

    Wang-Shick Ryu

    Article

    Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

    Insert

    1.3 units of the X-null HBV genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Tet-O-FUW-EGR3-P2A-mCherry

    Plasmid

    #203861

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR3 (EGR3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Inducible Caspex expression

    Plasmid

    #97421

    Purpose

    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

    Depositor

    Samuel Myers, Steven Carr

    Article

    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

    Inserts

    Caspex
    T2A-Gfp
    

    Use

    CRISPR

    Tags

    3xFLAG and V5

    Expression

    Mammalian

    Mutation

    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

    Promoter

    TRE3G

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. dCas9-GCN5 core

    Plasmid

    #179551

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR221_EGFP

    Plasmid

    #25899

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TUBG1_pLX307

    Plasmid

    #98376

    Purpose

    Lentiviral expression of TUBG1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    TUBG1 (TUBG1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV_BiVIPe4_Gq_dTomato

    Plasmid

    #213858

    Purpose

    AAV construct expressing HA-hM3Dq-dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_Gq_dTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    HA-GqDREADD-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Integrin-β6 I domain I270C

    Plasmid

    #159639

    Purpose

    Contains integrin beta 6 residues 108–352 with I270C mutation

    Depositor

    Timothy Springer

    Article

    Dong et al Nature. 2017 Feb 2;542(7639):55-59. doi:

    Insert

    Integrin beta 6 I domain I270C
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    I270C

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ROS lux sensor

    Plasmid

    #199791

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    ROS lux Sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCytERM-mBaoJin-N1

    Plasmid

    #229567

    Purpose

    Expresses P450-mBaoJin fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PIK3CA-SmBiT Fusion Vector

    Plasmid

    #237093

    Purpose

    Express PIK3CA-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. PIK3CA-LgBiT Fusion Vector

    Plasmid

    #237094

    Purpose

    Express PIK3CA-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. SmBiT-MDM2 Control Vector

    Plasmid

    #237037

    Purpose

    Express SmBiT-MDM2 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge