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Showing: 4961 - 5000 of 167150 results

  3. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  4. TFORF2857

    Plasmid
    #143642
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ID4 (ID4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143642-plasmid-map-sequence-id-396968

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  11. Lamp1_mStayGold

    Plasmid
    #221902
    Purpose
    Expresses mStayGold fused to Lamp1
    Depositor
    Janelia Integrative Imaging
    Insert
    Lamp1_mStayGold
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    221902-plasmid-map-sequence-id-438067

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  14. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140002-plasmid-map-sequence-id-275516

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  19. pCI-Puro_hCOL4A4-Myc-His

    Plasmid
    #198093
    Purpose
    Express hCOL4A4 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A4 (COL4A4 Human)
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198093-plasmid-map-sequence-id-389398

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  23. pGWB508-cTP-mCherry

    Plasmid
    #213862
    Purpose
    Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)
    Depositor
    Yutaka Kodama
    Article
    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.
    Insert
    Plastid transit peptide fused mCherry-UnaG
    Expression
    Bacterial and Plant
    Available Since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213862-plasmid-map-sequence-id-425330

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Showing: 4961 - 5000 of 167150 results