We narrowed to 8,907 results for: Ott

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pLL3.7

Plasmid

#11795

Purpose

3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

Depositor

Luk Parijs

Article

Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

Plasmid

#74309

Purpose

Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-NLRP3(R260W)

Plasmid

#73956

Purpose

Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

Depositor

Christian Stehlik

Article

Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

c-myc

Expression

Mammalian

Mutation

R260W

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-HRE-dUnaG

Plasmid

#124372

Purpose

UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

Depositor

Roland Friedel

Article

Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

Insert

HRE-dUnaG

Use

Lentiviral

Tags

PEST-degron and Myc tag

Promoter

HRE (HIF responsive element 5x + CMV minimal prom…

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1989

Plasmid

#49120

Purpose

AAV plasmid with CAGGS promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV_RTN3E-mScarlet3

Plasmid

#216255

Purpose

mammalian expression vector for RTN3E-mScarlet3

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

reticulon 3 isoform e (RTN3 Human)

Tags

mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2144

Plasmid

#104357

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

Insert

ssAAV-smCBA-EmGFP

Use

AAV

Promoter

smCBA

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Cterminal HA WDR59 pRK5

Plasmid

#46328

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR59 (WDR59 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pNCST-avGFP

Plasmid

#129508

Purpose

Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

avGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBF_HsPepT2

Plasmid

#73944

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT2 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT2 (SLC15A2 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pBF_HsPepT1

Plasmid

#73943

Purpose

Xenopus oocyte expression vector containing the cDNA of human PepT1 with a c terminal flag tag

Depositor

Simon Newstead

Article

Beale et al Structure. 2015 Oct 6;23(10):1889-99. do

Insert

PepT1 (SLC15A1 Human)

Use

Make rna for xenopus oocyte injection

Tags

Flag

Promoter

beta globin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

Plasmid

#113898

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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DYRK1A/pTRE3G-FL-hXIST

Plasmid

#149608

Purpose

Express doxycycline-inducible full length of human XIST cDNA

Depositor

Jeanne Lawrence

Article

Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

Insert

X-inactive specific transcript (XIST Human)

Expression

Mammalian

Promoter

pTRE3G

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-hCas3

Plasmid

#134920

Purpose

Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas3 with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO

Plasmid

#73311

Purpose

Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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p6347 MSCV-CMV-Flag-HA-Brd4-444-722

Plasmid

#31353

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 444-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pGAPTrap TALEN 2

Plasmid

#83369

Purpose

Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN2

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGAPTrap-TALEN1

Plasmid

#83368

Purpose

Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN1

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pAAV_hSyn1_nLightG

Plasmid

#187179

Purpose

Expresses green norepinephrine indicator nLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has Service

AAV9

Insert

nLightG

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-O2S

Plasmid

#21162

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4, Sox2 (SOX2, POU5F1 Human)

Use

Lentiviral; Reprogramming

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAX198

Plasmid

#173042

Purpose

Custom vector for sgRNA library construction

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_hSyn1_nLightR

Plasmid

#187180

Purpose

Expresses red norepinephrine indicator nLightR in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has Service

AAV9

Insert

nLightR

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag WDR24 pRK5

Plasmid

#46334

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Tags

FLag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAPTrap-GFP

Plasmid

#82506

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-mcBEST

Plasmid

#209416

Purpose

Plasmid for multiplexed cytosine base editing in streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

Inserts

Codon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1

Use

CRISPR and Synthetic Biology

Promoter

base editing cassette: PtipA ; sgRNAs: PkasO* an…

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1925

Plasmid

#105869

Purpose

High copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmid

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

iCreERT2

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY108 (lenti-AsCpf1)

Plasmid

#84739

Purpose

Lenti virus delivery of AsCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hAdipoq-GFP

Plasmid

#176215

Purpose

Expresses GFP under the control of distal enhancer and the promoter of human adiponectin gene

Depositor

Yu-Hua Tseng

Article

Shamsi et al Nat Commun. 2020 Mar 17;11(1):1421. doi:

Insert

GFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynapsin1.OxLight1

Plasmid

#169792

Purpose

Fluorescent reporter for orexin neuropeptides

Depositor

Tommaso Patriarchi

Article

Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

Insert

OxLight1

Use

AAV

Promoter

human Synapsin-1

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
6His-MBP-TEV-huAsCpf1

Plasmid

#90095

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pY109 (lenti-LbCpf1)

Plasmid

#84740

Purpose

Lenti virus delivery of LbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
hOT-GFP

Plasmid

#67848

Purpose

mammalian expression of human oxytocin receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

oxytocin receptor (OXTR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE

Plasmid

#67291

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Asc1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCST-AausFP1

Plasmid

#129499

Purpose

Expresses AausFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2158

Plasmid

#70119

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-EmGFP

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Flag Grb10 wt long

Plasmid

#37481

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 (GRB10 Human)

Tags

Flag

Expression

Mammalian

Mutation

isoform a, 594aa

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 8,907 results for: Ott