4,134 results

1. pRK5-HA GST RagB wt

   Plasmid

   #19301

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding B (RRAGB Human)
   

   Use

   Tags

   GST and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. hTLR3 flag

   Plasmid

   #13084

   Depositor

   Ruslan Medzhitov

   Insert

   TLR3 (TLR3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Amino acids 1-17 have been replaced by mammalian …

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPR::rpsL

   Plasmid

   #44505

   Purpose

   A crRNA expression plasmid specific to the rpsL allele.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR::rpsL (rpsL )
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPRIME-TET-GFP-FF3

   Plasmid

   #11662

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCV-Flag-hSox2

   Plasmid

   #20073

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   hSox2 (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-myc-LC3 (Atg8)

   Plasmid

   #24919

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   LC3 (MAP1LC3B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223-KDR

   Plasmid

   #23925

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   KDR (KDR Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   V297I

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MDH1-PGK-GFP_2.0

   Plasmid

   #11375

   Purpose

   Retroviral construct for miRNA expression

   Depositor

   Chang-Zheng Chen

   Article

   Chen et al Science. 2004 Jan 2. 303(5654):83-6.

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-CaMPARI W391F V398L

   Plasmid

   #60787

   Purpose

   Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI W391F V398L
   

   Use

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223-MET

    Plasmid

    #23889

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MET (MET Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-HA-VCPIP1

    Plasmid

    #22592

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    VCPIP1 (VCPIP1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. E[beta]P

    Plasmid

    #24313

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Sites phosphorylated by GSK-3beta have been mutat…

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-HA-USP42

    Plasmid

    #22603

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP42 (USP42 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-HA GST RagB 54L

    Plasmid

    #19302

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding B (RRAGB Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    T54L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

15. PX404 Campylobacter jejuni Cas9

    Plasmid

    #68338

    Purpose

    Campylobacter jejuni Cas9

    Depositor

    Feng Zhang

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 809 pcDNA3 GFP PTEN

    Plasmid

    #10759

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSicoR PGK puro

    Plasmid

    #12084

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pST1374-N-NLS-flag-linker-Cas9-D10A

    Plasmid

    #51130

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    D10A (nickase mutant)

    Promoter

    CMV

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQCXI Neo DsRed-LC3-GFP

    Plasmid

    #31183

    Depositor

    David Sabatini

    Article

    Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

    Insert

    DsRed-LC3-GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-HA-OTUD7B

    Plasmid

    #22550

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUD7B (OTUD7B Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-fSST-RFP

    Plasmid

    #22913

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    fugu somatostatin promoter driving DsRed 2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-rab9 DN

    Plasmid

    #12664

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    changed serine 21 to asparagine

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Flag-HA-CYLD

    Plasmid

    #22544

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    CYLD (CYLD Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCBFRE-luc

    Plasmid

    #26897

    Depositor

    Nicholas Gaiano

    Article

    Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

    Insert

    CBFRE
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pGEMTEZ-TeTxLC

    Plasmid

    #32640

    Depositor

    Joseph Gogos, C. Ron Yu, Richard Axel

    Article

    Yu et al Neuron. 2004 May 27;42(4):553-66.

    Insert

    Tetanus toxin light chain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pQC NLS mCherry IX

    Plasmid

    #37354

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    NLS-mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. aMHC-eGFP-Rex-Neo

    Plasmid

    #21229

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV.ATMi

    Plasmid

    #14542

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    ATM shRNA (ATM Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBiFC-bFOSDelataZipVC155

    Plasmid

    #22014

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bFosDelataZIPVC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of residues 179-193 in bFos(118-211)

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

    Plasmid

    #12153

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge