We narrowed to 18,983 results for: INO

1. pMarsL274G

   Plasmid

   #63177

   Purpose

   This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

   Depositor

   David Tirrell

   Article

   Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

   Insert

   MarsL274G (Mars1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L274G

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-RNF2

   Plasmid

   #126544

   Purpose

   Expresses GFP-tagged RNF2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RING1B (RNF2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdest EGFP Neo

   Plasmid

   #223052

   Purpose

   EGFP gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNTI194

   Plasmid

   #84266

   Purpose

   Expresses Avi-tagged RPL1 and humanized birA

   Depositor

   Nicholas Ingolia

   Article

   Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

   Inserts

   RPL1 (RPL10A Human, (*see below))
   humanized birA
   

   Use

   Tags

   Avi tag, Flag, Gly/Ser linker, and TEV site

   Expression

   Mammalian

   Mutation

   R33A, A34E (*see below)

   Promoter

   CMV and P2A peptide

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-ataxin-3

   Plasmid

   #89975

   Purpose

   Expresses GFP-tagged ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

   Plasmid

   #208963

   Purpose

   Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2Cre

   Plasmid

   #82415

   Purpose

   Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

   Depositor

   David Feldser

   Article

   Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

   Insert

   Cre Recombinase
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Mutated BsmBI cut site

   Promoter

   EFS (P2A)

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX307 MCL1

   Plasmid

   #117726

   Purpose

   Open reading frame vector encoding MCL1

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   MCL1L (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TetO-FUW-NfiA

   Plasmid

   #64901

   Purpose

   Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

   Depositor

   Vania Broccoli

   Article

   Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

   Insert

   Nfia (Nfia Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF5/FRT/DEST-3xHA/mGli2 P1-6A

    Plasmid

    #51251

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 789,805,817,848,923,956 mutated to Ala

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-nEF-ChRmine-mScarlet

    Plasmid

    #137158

    Purpose

    Viral expression of ChRmine-p2a-mScarlet

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    ChRmine-p2a-mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29b-rhMOG

    Plasmid

    #225484

    Purpose

    Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

    Depositor

    John Pak

    Article

    Wu et al J Immunol Methods. 2024 Oct 13:113764. d

    Insert

    Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-29 (signal peptide) and 150…

    Promoter

    T7

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE puro SDF-1 alpha

    Plasmid

    #12270

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    SDF-1 alpha (CXCL12 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pColdI-DDX3_helicase_core

    Plasmid

    #233618

    Purpose

    Expression of His-tagged helicase core region of DDX3X in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    DDX3X helicase core WT (DDX3X Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TetO-FUW-NfiB

    Plasmid

    #64900

    Purpose

    Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    NfiB (Nfib Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-FBXL10

    Plasmid

    #126542

    Purpose

    Expresses GFP-tagged FBXL10 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    JHDM1B (KDM2B Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-MCP-SRSF3-V5-mCherry

    Plasmid

    #235090

    Purpose

    SRSF3 mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-SRSF3-V5-mCherry (SRSF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

    Plasmid

    #208046

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Strep-His-Tev-hCGN

    Plasmid

    #236512

    Purpose

    For expression of Cingulin (human) in insect cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    CGN (CGN Human)
    

    Use

    Tags

    2xStrep-10xHis-Tev

    Expression

    Insect

    Mutation

    WT

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

    Plasmid

    #114112

    Purpose

    fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    NanoLuc; Volvox channelrhodopsin 1; EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMSCV-IRES-GFP/CREB3L1

    Plasmid

    #214767

    Purpose

    Mammalian expression of human CREB3L1

    Depositor

    Norio Komatsu

    Article

    De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

    Insert

    CREB3L1 (CREB3L1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA-hDDB2-WT-V5

    Plasmid

    #167468

    Purpose

    ectopic expression of hDDB2 in mammalian cells

    Depositor

    Steven Artandi

    Article

    Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

    Insert

    DDB2 (DDB2 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBK- Ma PylRS WT

    Plasmid

    #197573

    Purpose

    Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Insert

    M. alvus PylRS WT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    GlnS (constitutive)

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

    Plasmid

    #221551

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-H2B-tdTomato-SRT

    Plasmid

    #203393

    Purpose

    AAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

    Insert

    H2B fused piggyBac tdTomato self-reporting transposon
    

    Use

    AAV

    Tags

    3X SV40 and H2B

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYI2165

    Plasmid

    #228347

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
    

    Use

    Affinity Reagent/ Antibody

    Tags

    AAA linker followed by FLAG-His6 and MF_ secretio…

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FGA-bio-His

    Plasmid

    #52029

    Purpose

    Expresses full-length Fibrinogen alpha chain precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    FGA (FGA Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmCherry-N1-GalT

    Plasmid

    #87327

    Purpose

    To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

    Depositor

    Lei Lu

    Article

    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

    Insert

    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

    Plasmid

    #178528

    Purpose

    Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    rtTA, SspB-mScarlet-I-MYPT (1-169aa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, Tet responsive element

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 8xNFAT-ZsG-muhCD4

    Plasmid

    #162745

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Human CD4 gene with glutamine to tyrosine at posi…

    Promoter

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET41b+_Ufd1-His

    Plasmid

    #117107

    Purpose

    Production of recombinant Ufd1-His in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Ufd1 (UFD1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. STARR-seq luciferase validation vector_ORI_CMV

    Plasmid

    #99312

    Purpose

    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    CMV enhancer
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-mGold2s-Calnexin-N-14

    Plasmid

    #231768

    Purpose

    Visualization of ER in mammalian cells using mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s-Calnexin-N-17 (CANX Human)
    

    Use

    Tags

    mGold2s

    Expression

    Mammalian

    Mutation

    I474N

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. piRFP670-N1-GalT

    Plasmid

    #87325

    Purpose

    To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

    Depositor

    Lei Lu

    Article

    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

    Insert

    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    

    Use

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

    Plasmid

    #214732

    Purpose

    Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

    Depositor

    Félix Leroy

    Article

    de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

    Insert

    anti-Crh shRNA / mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET41b+_Npl4

    Plasmid

    #117108

    Purpose

    Production of recombinant Npl4 in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Npl4 (NPLOC4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAc-C Med12His

    Plasmid

    #49240

    Purpose

    expresses human Med12 with His tag in insect cells

    Depositor

    Dylan Taatjes

    Article

    Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

    Insert

    Med12 (MED12 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. STARR-seq luciferase validation vector_ORI_SV40

    Plasmid

    #99309

    Purpose

    Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    SV40 enhancer
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p3xFLAG-CMV14-GINIP

    Plasmid

    #204845

    Purpose

    Express 3x FLAG tagged-GINIP in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

    Insert

    PHF24 (KIAA1045 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pWAP-HGF

    Plasmid

    #83503

    Purpose

    Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

    Depositor

    Marta Gallego, Lothar Hennighausen

    Article

    Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

    Insert

    HGF (Hgf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    also contains beta-globin sequences from pUC198

    Promoter

    mouse WAP (whey acidic protein)

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GFP-PH-TAPP1

    Plasmid

    #161985

    Purpose

    Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

    Depositor

    David Bryant, Dario Alessi

    Article

    Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

    Insert

    PH-TAPP1 (PLEKHA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAVTRE3_Clover

    Plasmid

    #135179

    Purpose

    AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Inserts

    Clover
    TRE3G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    a variant of GFP

    Promoter

    TRE3G

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. STARR-seq luciferase INF enhancer vector_ORI_MX1

    Plasmid

    #99323

    Purpose

    Luciferase validation vector with MX1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    enhancer; chr21: 42791443 -42793515
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pet16b-Avi-ubiquitin_rbs_BirA

    Plasmid

    #134897

    Purpose

    Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Inserts

    Ubiquitin (UBB Human)
    BirA
    

    Use

    Tags

    10HIS-AviTag-PreScission(3C)protease

    Expression

    Bacterial

    Mutation

    Promoter

    RBS and T7

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pTwist-SARS-CoV Δ18

    Plasmid

    #169465

    Purpose

    Encodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAcBac1-EcTrp-TGA

    Plasmid

    #228780

    Purpose

    aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTrpRS
    EcWtR
    

    Use

    Baculoviral

    Tags

    Expression

    Mammalian

    Mutation

    TGA suppressor

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET30a-His6-ABP-LOXCAT

    Plasmid

    #139971

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. AAV-SYP1-miniSOG-T2A-mCherry

    Plasmid

    #50972

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)
    

    Use

    AAV

    Tags

    T2A-mCherry and miniSOG

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. mEGFP-PI35P2

    Plasmid

    #92419

    Purpose

    Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

    Insert

    MCOLN1 (Mcoln1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Synthetic gene, codon optimized for human.

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLX209-neo-HA-Ub-T2A-Tet3G

    Plasmid

    #202436

    Purpose

    Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    Ubiquitin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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