Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,560 results for: chia

Showing: 5481 - 5520 of 5560 results

  1. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Ryan Mehl, Richard Cooley
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

    Enlarge

  2. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

    Enlarge

  4. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

    Enlarge

  5. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

    Enlarge

  6. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid
    #134924
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134924-plasmid-map-sequence-id-267600

    Enlarge

  7. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid
    #196185
    Purpose
    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Use
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    changed Serine 9 to Alanine
    Promoter
    Tac
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196185-plasmid-map-sequence-id-383261

    Enlarge

  8. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid
    #134923
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134923-plasmid-map-sequence-id-267593

    Enlarge

  9. pPB-tetpA(hBCL2-miR9/9*-124)-iRPT

    Plasmid
    #154881
    Purpose
    Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.
    Depositor
    Hideyuki Okano
    Article
    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001
    Inserts
    iRFP713
    Puromycin resistance gene
    rtTAM2
    BCL2 (BCL2 Human)
    miR9/9*-124
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    154881-plasmid-map-sequence-id-313227

    Enlarge

  10. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid
    #122085
    Purpose
    Express M.EcoGII fused to CENPC with destabilization domain - inducible
    Depositor
    Laure Crabbe
    Article
    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5
    Insert
    DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
    Use
    Retroviral
    Tags
    V5 tag on Nter of CENPC
    Expression
    Bacterial and Mammalian
    Mutation
    L341F, R930L (see comments below)
    Promoter
    Available since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122085-plasmid-map-sequence-id-238127

    Enlarge

  11. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187950
    Purpose
    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187950-plasmid-map-sequence-id-373501

    Enlarge

  13. 3'-ecRESCUE-mCherry

    Plasmid
    #191486
    Purpose
    ecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: TMP-induced ecDHFR DD expression allows reversible control of the expression of dRanCas13b-ADAR2-mCherry
    Depositor
    Xu Ma
    Article
    Wang et al Cell Commun Signal. 2021 Jul 31;19(1):81
    Insert
    dRanCas13b-hADAR2-mCherry-ecDHFR (folA, ADARB1 R. anatipestifer, E. coli, Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    191486-plasmid-map-sequence-id-374351

    Enlarge

  14. pCIBN-LacI-tagRFP-T

    Plasmid
    #103810
    Purpose
    'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-LacI-tagRFP-T (CIB1, lacI E. coli, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    LacI: C19T (silent, L7L), T264C (silent, A88A), C…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103810-plasmid-map-sequence-id-199266

    Enlarge

  15. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid
    #170808
    Purpose
    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
    Depositor
    Kenneth Yamada
    Article
    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.
    Inserts
    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Synthetic, S. aureus, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Synonymous mutation in PYL1 to remove XhoI site (…
    Promoter
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    170808-plasmid-map-sequence-id-334406

    Enlarge

  16. WT MetNIQ in pBAD

    Plasmid
    #118253
    Purpose
    Wild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala)
    Promoter
    None and araBAD Promoter
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    118253-plasmid-map-sequence-id-226828

    Enlarge

  18. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2

    Plasmid
    #217368
    Purpose
    Expresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAV
    Depositor
    Abhishek Chatterjee
    Article
    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2
    Insert
    E. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    C2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83C
    Promoter
    U6
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    217368-plasmid-map-sequence-id-430295

    Enlarge

  19. pOET3-6xHis-hSMSr-ΔSAMD

    Plasmid
    #236225
    Purpose
    Express N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cells
    Depositor
    Chiaki Murakami
    Article
    Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do
    Insert
    SAMD8 (SAMD8 Human)
    Use
    Tags
    Hexa histidine tag (6xHis-tag) and enterokinase s…
    Expression
    Insect
    Mutation
    sterile alpha motif domain at N-terminal (1-231 b…
    Promoter
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236225-plasmid-map-sequence-id-471035

    Enlarge

  21. pPICZ:K2P2.1(mTREK-1)Cryst S112C

    Plasmid
    #224780
    Purpose
    Pichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter
    Depositor
    Dan Minor
    Article
    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1
    Insert
    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    Use
    Tags
    Expression
    Yeast
    Mutation
    K84R / Q85E / T86K / I88L / A89R / Q90A / A92P / …
    Promoter
    AOX1 promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224780-plasmid-map-sequence-id-445194

    Enlarge

  22. pNK845

    Plasmid
    #219746
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219746-plasmid-map-sequence-id-437111

    Enlarge

  23. pAAV-syn-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234437
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234437-plasmid-map-sequence-id-465724

    Enlarge

  24. pAAV-syn-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234438
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234438-plasmid-map-sequence-id-465745

    Enlarge

  25. pAAV-CAG-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234451
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234451-plasmid-map-sequence-id-465732

    Enlarge

  26. pAAV-CAG-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234452
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234452-plasmid-map-sequence-id-465722

    Enlarge

  28. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

    Enlarge

  30. pAAV-CAG-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234450
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234450-plasmid-map-sequence-id-465729

    Enlarge

  31. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234436
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234436-plasmid-map-sequence-id-465719

    Enlarge

  32. pAAV-syn-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234435
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234435-plasmid-map-sequence-id-465726

    Enlarge

  33. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

    Plasmid
    #230043
    Purpose
    Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.
    Depositor
    Sumeet Pal Singh
    Article
    Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:
    Insert
    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
    Use
    Cre/Lox; Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    fabp10a
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    230043-plasmid-map-sequence-id-461059

    Enlarge

  34. pAAV-GFAP-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234445
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    GFAP
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234445-plasmid-map-sequence-id-465739

    Enlarge

  35. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234446
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    GFAP
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234446-plasmid-map-sequence-id-465727

    Enlarge

  38. pAav-TP53-T2A-BirA*

    Plasmid
    #115652
    Purpose
    rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette
    Depositor
    Sven Eyckerman
    Article
    Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102
    Inserts
    TP53 (TP53 Human)
    TP53 (TP53 Human)
    T2A
    BirA*
    Use
    AAV
    Tags
    BirA* and T2A
    Expression
    Mutation
    Homology region 1 and Homology region 2
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    115652-plasmid-map-sequence-id-223051

    Enlarge

  40. pLY54

    Plasmid
    #130923
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130923-plasmid-map-sequence-id-257307

    Enlarge

Showing: 5481 - 5520 of 5560 results