We narrowed to 16,886 results for: Por

1. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

   Plasmid

   #12153

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor (RHOA Human)
   

   Use

   Retroviral

   Tags

   6XHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAL149

   Plasmid

   #22275

   Purpose

   Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB(1-908) (PHYB Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   mCherry-Kras4BCT (prenylation sequence)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

   Plasmid

   #177265

   Purpose

   STREAMING-tag cassette

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag cassette
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAVS1-Puro-CAG-GCaMP6s

   Plasmid

   #120896

   Purpose

   Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

   Depositor

   Xiaojun Lian

   Article

   Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

   Insert

   GCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TetO-FUW-Rfx4

   Plasmid

   #61544

   Purpose

   Expresses Rfx4 in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   Rfx4 (Rfx4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pRK5-HA-SLC38A9.1

   Plasmid

   #71869

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-myc-FLNa S2152A

   Plasmid

   #8983

   Depositor

   John Blenis

   Article

   Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

   Insert

   Filamin A S2152A (FLNA Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Ser2152Ala

   Promoter

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EGFP-uTEV1-CB5

   Plasmid

   #171007

   Purpose

   HiLITR protease with CB5 C-terminal tmd targeting information (ER)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-CB5(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJJR82

   Plasmid

   #75027

   Purpose

   EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Article

   Ramalho et al Development. 2020 Jul 22;147(14):dev1880

   Insert

   EGFP^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized EGFP

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MCU-mCherry

    Plasmid

    #232790

    Purpose

    Encodes an mCherry-tagged MCU/Mitochondrial Calcium Uniporter

    Depositor

    Giulia Bertolin

    Article

    Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

    Insert

    Calcium uniporter protein, mitochondrial (MCU )
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV_CAG_V5-LOV-Turbo-NES

    Plasmid

    #199670

    Purpose

    expresses V5-LOV-Turbo-NES in mammalian cells, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    AAV

    Tags

    NES and V5

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TetO-FUW-PBRM1-pgk-puro

    Plasmid

    #85746

    Purpose

    tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

    Depositor

    Emily Dykhuizen

    Article

    Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Hsp70-mplum

    Plasmid

    #45016

    Depositor

    Anna Planas

    Article

    de la Rosa et al Eur J Nucl Med Mol Imaging. 2013 Feb;40(

    Insert

    mPlum
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Hspa1b

    Available Since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-JNK2

    Plasmid

    #86831

    Purpose

    mammalian expression of GFP-JNK2

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    JNK2 (MAPK9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174883

    Purpose

    Targeting vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Pou5f1
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221_SLC6A3

    Plasmid

    #132160

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Sijben et al Sci Rep. 2021 Jan 14;11(1):1312. doi: 10

    Insert

    SLC6A3 (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. CaTCH BC1

    Plasmid

    #154195

    Purpose

    Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    CaTCH BC1
    

    Use

    Lentiviral; Catch barcode labeling

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Use

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.2-DEST-MatryoshCaMP6s

    Plasmid

    #100025

    Purpose

    Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Use

    Tags

    V5 Epitope Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTriEx-RhoA FLARE.sc Biosensor Q63L

    Plasmid

    #12151

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor Q63L (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA Q63L (dominant-positive mutation)

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CaTCH_empty-backbone

    Plasmid

    #157746

    Purpose

    Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral; Catch barcode cloning

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-MyosinVa-Ctail

    Plasmid

    #110169

    Purpose

    Dominant negative effect by competing with MyosinVa C-terminal tail interactions

    Depositor

    Jose Esteban

    Article

    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

    Insert

    Myosin Va (Myo5a Mouse)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    amino acids 1444-1853

    Promoter

    CMV

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-h-mmPtch1-B-HA-TagBFP

    Plasmid

    #120910

    Purpose

    Expresses BFP tagged Ptch1-B

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-ROS1-V5/HIS

    Plasmid

    #205609

    Purpose

    Expression of human ROS1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROS1 receptor tyrosine kinase (ROS1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTDpelB-NTwinStrep

    Plasmid

    #45940

    Purpose

    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis, PelB, and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-hGFAP-Pink Flamindo-WPRE-SV40

    Plasmid

    #178790

    Purpose

    AAV vector to express the red cAMP indicator in astrocytes

    Depositor

    Tetsuya Kitaguchi

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    Pink Flamindo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hGFAP

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. tet_pLKO.1_puro_shLuc

    Plasmid

    #136587

    Purpose

    Expresses an inducible short hairpin targeting firefly luciferase sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEVOL-NBK-1

    Plasmid

    #207639

    Purpose

    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The evolved synthetase NBK-1 contains mutations Y…

    Promoter

    araBAD

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB-TRE3G-SOX17

    Plasmid

    #104541

    Purpose

    PiggyBac vector encoding doxycycline-inducible human SOX17

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    SRY-box 17 (SOX17 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3/hArf5(T31N)-HA

    Plasmid

    #79429

    Purpose

    Expresses C-terminally HA-tagged Arf5(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 31 to Asparagine

    Promoter

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3/hArf5(Q71L)-HA

    Plasmid

    #79428

    Purpose

    Expresses C-terminally HA-tagged Arf5(Q71L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 71 to Leucine

    Promoter

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTriEx-RhoA FLARE.sc Biosensor T19N

    Plasmid

    #12152

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor T19N (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA T19N (dominant-negative mutation)*

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

    Plasmid

    #86194

    Purpose

    beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

    Depositor

    Robert Singer

    Article

    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

    Insert

    beta Globin (HBB, Hbb Human, Mouse)
    

    Use

    Tet on

    Tags

    Expression

    Mammalian

    Mutation

    39TER in beta Globin in MCS II

    Promoter

    Tet on

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid

    #124434

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq) T2A mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Mito-LOV-Turbo-V5

    Plasmid

    #199666

    Purpose

    expresses LOV-Turbo in the mammalian mitochondria, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    COX4I1 mito targeting sequence and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEF-ABEmax

    Plasmid

    #164415

    Purpose

    EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    ABEmax
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGL3-basic-hRARβP-FL-luciferase

    Plasmid

    #135447

    Purpose

    Human full-length promoter of retinoic acid receptor-beta gene in pGL3-Basic-luciferase plasmid vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    Full-length promoter of human retinoic acid receptor-beta gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Human RARβ promoter

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174882

    Purpose

    Targeting vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Nanog
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMKO.1 puro p53 shRNA 2

    Plasmid

    #10672

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pmTurqoise KIF1A shRNA

    Plasmid

    #102849

    Purpose

    Generates shRNA specific to Rat KIF1A along with cell fill pmTurqoise

    Depositor

    Erik Dent

    Article

    McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

    Insert

    KIF1A-shRNA (KIF1A Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1-RNA

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAG-SpCas9-2A-GFP-noITR

    Plasmid

    #118415

    Purpose

    Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTrc99A-Rat FABP1

    Plasmid

    #13577

    Depositor

    Martin Scanlon

    Article

    Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

    Insert

    Rat liver fatty acid binding protein (Fabp1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

43. pCS2 Notch1 ΔEMV-6MT

    Plasmid

    #41737

    Depositor

    Jeffrey Nye, Raphael Kopan

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Extracellular-domain-deleted construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 protein fragment lacking …

    Promoter

    Available Since

    Jan. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRR-ERbeta-5Z

    Plasmid

    #23062

    Depositor

    Charles Miller

    Article

    Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

    Insert

    ERbeta (ESR2 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

45. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA3.1-GNPTAB

    Plasmid

    #78108

    Purpose

    Mammalian expression of human GNPTAB with C terminal myc tag

    Depositor

    Thomas Braulke

    Article

    Franke et al J Biol Chem. 2013 Jan 11;288(2):1238-49.

    Insert

    GNPTAB (GNPTAB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

47. TetO-FUW-VdC9BV

    Plasmid

    #62195

    Purpose

    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

    Depositor

    Kam Leong

    Article

    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

    Insert

    VP64dCas9BFPVP64
    

    Use

    Lentiviral

    Tags

    Two VP64s tagged to dCas9 fused to BFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive) Cas9 (dCas9)

    Promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET-51b(+)-Caprola_13

    Plasmid

    #194674

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_13
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pET24a-VHH-tev-mCherry

    Plasmid

    #117752

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET-51b(+)-cpHaloTag141/145

    Plasmid

    #194660

    Purpose

    Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHaloTag141/145
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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