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161,641 results

Showing: 7001 - 7050 of 161641 results

  1. pBWB514

    Plasmid
    #209326
    Purpose
    p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.
    Depositor
    Adam Arkin
    Article
    Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/
    Insert
    mRFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tet
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    209326-plasmid-map-sequence-id-414115

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  2. pCambia-dLB-kanR-GFP

    Plasmid
    #223177
    Purpose
    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border
    Depositor
    Andreas Stuermer
    Type
    Empty backbone
    Use
    Ti plasmid for agrobacterium
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    223177-plasmid-map-sequence-id-443110

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  8. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100539-plasmid-map-sequence-id-194476

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  9. pSBbi-pur H-2Kb

    Plasmid
    #111623
    Purpose
    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele
    Depositor
    Jon Yewdell
    Insert
    H2Kb
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111623-plasmid-map-sequence-id-213220

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  10. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid
    #183532
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183532-plasmid-map-sequence-id-362037

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  13. pRK5-myc-Rac1-Q61L

    Plasmid
    #12983
    Depositor
    Gary Bokoch
    Insert
    Rac1 constitutively active (RAC1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q61L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12983-plasmid-map-sequence-id-154551

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  16. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid
    #105921
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs
    Depositor
    Marcella Patrick
    Has Service
    AAV2
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Tags
    Expression
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    105921-plasmid-map-sequence-id-443051

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  20. pVC-FUS-R521C

    Plasmid
    #175067
    Purpose
    expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Use
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    changed Arginin 521 to Cystein; mVenus: C-Termina…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175067-plasmid-map-sequence-id-362347

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  21. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid
    #177328
    Purpose
    Control vector for Flp-dependent DREADD constructs
    Depositor
    Ulrik Gether
    Insert
    mCherry
    Use
    AAV; Flp-frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177328-plasmid-map-sequence-id-349583

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  25. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #161928
    Purpose
    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    Surface marker and Citrine activation reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    161928-plasmid-map-sequence-id-325287

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  27. TFORF2161

    Plasmid
    #141950
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GRHL1 (GRHL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141950-plasmid-map-sequence-id-309802

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  29. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Article
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

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  45. pAAV-Syn-Chronos-tdTomato

    Plasmid
    #62726
    Purpose
    AAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV8
    Insert
    Chronos-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin promoter
    Available since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62726-plasmid-map-sequence-id-195120

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  46. minCMV-1xmNG-dMCP

    Plasmid
    #231091
    Purpose
    Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).
    Depositor
    John Ngo
    Insert
    1xmNG-dMCP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231091-plasmid-map-sequence-id-458492

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  48. pCMV-CreEGFP

    Plasmid
    #165140
    Purpose
    Expression of Cre recombinase fused with EGFP in C-terminal
    Depositor
    Daekee Lee, David Threadgill
    Insert
    Cre recombinase
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    165140-plasmid-map-sequence-id-326583

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  50. pJAT17

    Plasmid
    #204298
    Purpose
    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-EYFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204298-plasmid-map-sequence-id-406176

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Showing: 7001 - 7050 of 161641 results