159,352 results
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pYL322d1/N-Vn
Plasmid#183153PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-FTH1
Plasmid#100749PurposePart of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tagDepositorInsertferritin heavy chain 1 (FTH1 Human)
UseTagsFRB and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-ABE8.20-NG
Plasmid#226866PurposeExpresses dox-inducible ABE8.20-NG for mammalian cells. piggyBac vectorDepositorInsertABE8.20-NG
UseLentiviralTagsExpressionMutationPromoterTet-On-TRE3GAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF3431
Plasmid#144907PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR3C2 (NR3C2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpe
Plasmid#13787PurposeMammalian expression of enhanced form of Flp recombinaseDepositorInsertFlpe
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
Plasmid#61762PurposeDual Color betaGlobin reporter to study splicing kinetics in vivoDepositorInsertb-Globin
UseTags24 X MS2 hairpin cassette in 3'UTR of b-Glob…ExpressionMammalianMutationPromoterDox inducible Tet-ON promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
3XMEF2-luc
Plasmid#32967PurposeReporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoterDepositorInsertMEF2 binding sites (MEF2A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterMEF2Available sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHEE401E
Plasmid#71287PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 enhancer fused to EC1.1 promoter and U6-26p…Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_DUGV_GP
Plasmid#207268PurposeMammalian expression of Dugbe virus GP proteinDepositorInsertDUGV_GP
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR7-DuET
Plasmid#213224PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR7 (ACKR3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2603
Plasmid#143373PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF471 (ZNF471 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-Cetn1
Plasmid#50717PurposeCetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.DepositorInsertCetn1 (Cetn1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_4D5-WT-Highest-CAR_RHL004
Plasmid#164826PurposeLentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CARDepositorInsertAntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV PIG (Puro IRES GFP empty vector)
Plasmid#21654PurposeRetroviral backbone for cloning and gene expression. Select with puromycin or GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pMH-SFB-DYRK1A
Plasmid#101770PurposeMammalian expression of DYRK1A fusion proteinDepositorInsertDYRK1A (DYRK1A Human)
UseTagsS peptide-flag-streptavidin binding peptide (SFB)ExpressionMammalianMutationsiRNA-resistant allelePromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
CALM-pmCherryN1
Plasmid#27691DepositorInsertCALM (Picalm Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGWB502-omega
Plasmid#74845PurposeGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter2xCaMV35S enhancer and omegaAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF0040
Plasmid#143684PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF676 (ZNF676 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2FLAG
Plasmid#16331DepositorInsertFlag tag
UseVertebrate expressionTagsFlagExpressionMutationFlag tag inserted into pCS2+ polylinkerPromoterAvailable sinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-pBAD
Plasmid#54572PurposeLocalization: Protein Expression Vector , Excitation: 399, Emission: 456DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, T7, and mTagBFP2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-Klb-EGFP
Plasmid#45531DepositorInsertbeta Klotho (Klb Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEG302-SunTag-FWAgRNA-4-22aa-TET1cd
Plasmid#115344PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
iRFP-Sec61beta
Plasmid#108125PurposeER markerDepositorInsertiRFP:SEC61B (SEC61B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_hygro-tagBFP-lox5171
Plasmid#162070Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b-CPDSalI
Plasmid#38251DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G2
Plasmid#40175DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm
Plasmid#63121Purposemini-Tn7 vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRU295
Plasmid#167694PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT006
Plasmid#64761PurposeRecombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEvoltac-EcW-TGA-h14
Plasmid#218764Purposeencodes EcWRS-h14 and EcW-tRNA-TGADepositorInsertEcWRS-h14
UseTagsExpressionBacterialMutationS8A, V144G, V146CPromotertacIAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3XFLAG-wtGATA6-3XAU1 neo
Plasmid#72618PurposeExpresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationPromoterTRE TightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV4Neo-murine IL-17RA-HA
Plasmid#46859Purposeexpresses mouse IL-17RADepositorInsertIL-17RA (Il17ra Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-miRFP704nano
Plasmid#206190PurposeNear-infrared fluorescent protein miRFP704nano in mammalian plasmidDepositorInsertmiRFP704nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB578
Plasmid#141095PurposeE. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat arrayDepositorInsertsCas9 and tracrRNA
repeat-spacer-repeat array
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2593
Plasmid#142336PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBHLHE40 (BHLHE40 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_TCF3_HLF
Plasmid#205935PurposeExpress mEGFP-tagged fusion protein, TCF3_HLF from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
AiP15043 - pAAV-AiE1419m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5043)
Plasmid#224189PurposeAiE1419m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neuronsInsertSYFP2-P2A-3XFLAG-10aa-H2B
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)
Plasmid#117168PurposeCRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1
Plasmid#187120Purposemammalian cell expression of FLAG-HUWE1DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX459v3
Plasmid#178799PurposeSpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG26
Plasmid#35127DepositorInserthph
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
1B-GFP1-10
Plasmid#193867PurposeE. coli expression vector for Hisx6 tagged GFP1-10.DepositorInsertsfGFP1-10
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
dSpyCas9-p300-P2A-CASANOVA
Plasmid#113129PurposeCo-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVADepositorInsertdCas9-p300-P2A-CASANOVA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-VSVG
Plasmid#1733PurposeVSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan labDepositorInsertVSV-G Envelope
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
HA-14-3-3 eta
Plasmid#116887PurposeExpresses HA-tagged human 14-3-3 eta in mammalian cellsDepositorInsert14-3-3 eta (YWHAH Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-AHPH-DM
Plasmid#71368PurposeLocation sensor for RhoA-GTPDepositorInsertC terminus of Anillin (ANLN Human)
UseTagsEGFPExpressionMammalianMutationA740D and E758KPromoterCMVAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only
