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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM60-S6K1(wt) barcoded

Plasmid

#48801

Purpose

expresses wt S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pXGFPC-5 Pxyl-dcas9 (Sth3)

Plasmid

#133317

Purpose

expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus thermophilus #3)

Use

Unspecified

Promoter

xylose

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMN-285

Plasmid

#195472

Purpose

Lentiviral Integratable Inducible CD19CAR using synZiFTR ZF10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF10 BS - ybTATA - CD19 CAR - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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mOtop3_pGEMHE

Plasmid

#114676

Purpose

This construct was used to express mouse Otop3 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 3 (Otop3 Mouse)

Use

For generating the mrna, which can highly express…

Promoter

T7

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENO1-iRFP-NATr

Plasmid

#129731

Purpose

Integration plasmid to overexpress iRFP in Candida albicans

Depositor

Robert Wheeler

Article

Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

Insert

iRFP-Candida albicans codon-optimized

Expression

Yeast

Promoter

ENO1 from Candida albicans

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_BIRC6

Plasmid

#197974

Purpose

Entry clone for wt BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Other

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRG659_lexO-FseI_LexA-TF_LEU2MX

Plasmid

#154816

Purpose

Inducible expression of FseI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

FseI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCBI-LexA-CIB1-biLINuS

Plasmid

#192605

Purpose

Plasmid for constitutive expression of LexA-CIB1-biLINuS.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCAGGS

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1

Plasmid

#32466

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

Expression

Bacterial

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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CAG_mCh_p85a_mCh

Plasmid

#199630

Purpose

FRET acceptor for IGF1R sensor

Depositor

Ryohei Yasuda

Article

Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

Insert

p85a-SH2 (Pik3r1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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tol2-CAG::ChR2-YFP

Plasmid

#59740

Purpose

Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Channelrhodopsin-2

Tags

YFP

Expression

Mammalian

Mutation

H134R

Promoter

CAG

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tet3 CD Mut

Plasmid

#72222

Purpose

Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 3

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET3 catalytic domain (cata…

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pGL3-Gateway-DSCP

Plasmid

#71506

Purpose

flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pJMP8930

Plasmid

#220905

Purpose

MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET26_MraYaa

Plasmid

#100166

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Chung et al Science. 2013 Aug 30;341(6149):1012-1016

Insert

mraY (phospho-MurNAc-pentapeptide translocase)

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHS_235

Plasmid

#195460

Purpose

Lentiviral Integratable, 4OHT/TMX-inducible expression of synZiFTR: ZF3-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - ZF3 - p65 - ERT2

Use

Lentiviral

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPM1001

Plasmid

#167548

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Inaba serotype

Use

Cosmid

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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p2H12

Plasmid

#221142

Purpose

cdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen

Expression

Bacterial

Promoter

Ptet

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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GST-NDP52

Plasmid

#171422

Purpose

For the purifcation of NDP52 protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

NDP52 (CALCOCO2 Human)

Expression

Bacterial

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pHLP710

Plasmid

#203740

Purpose

Integration of OsTIR1-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Promoter

ACT1 (C. albicans)

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDFDuet-trAtCCD7-OsD27(pYL726 )

Plasmid

#178285

Purpose

Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

OsD27
trAtCCD7

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shACO1

Plasmid

#169884

Purpose

Suppress ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

shRNA against ACO1

Use

Lentiviral and RNAi

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJ823

Plasmid

#39313

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (lin2744 Listeria innocua)

Use

CRISPR

Tags

MBP and thioredoxin-His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only
SG3ΔENV CA M66I

Plasmid

#149687

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residue Met 66 to Iso (CA M66I)

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mOtop2_pGEMHE

Plasmid

#114675

Purpose

This construct was used to express mouse Otop2 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 2 (Otop2 Mouse)

Use

For generating the mrna, which can highly express…

Promoter

T7

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mOtop2_pcDNA3

Plasmid

#114678

Purpose

This construct was used to express mouse Otop2 in HEK-293 cells

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 2 (Otop2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
C1-Lyn-mCitrine-EEVEE-LifeAct

Plasmid

#155226

Purpose

Alternate MPAct using Lyn/LifeAct instead of CaaX/F-tractin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

Plasmid

#133339

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT3-FLAG-KLF3

Plasmid

#49102

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Himeda et al Mol Cell Biol. 2010 Jul;30(14):3430-43.

Insert

Krüppel-Like Factor 3(KLF3)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIS18-BsaI

Plasmid

#128649

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mGold-Tubulin-C-18

Plasmid

#158008

Purpose

Visualization of tubulin in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Tubulin (TUBA1B Human)

Tags

mGold

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUB_smFLAG_H2B_MS2

Plasmid

#81082

Purpose

Translation reporter: Encodes Histone H2B (125 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_H2B_24xMS2

Expression

Mammalian

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-BoNT/E

Plasmid

#168020

Purpose

Expresses BoNT/E-LC(1-411)-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

BoNT/E-LC(1-411)-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSSN6

Plasmid

#104340

Purpose

Express SSN6

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

SSN6 (CYC8 Budding Yeast)

Expression

Yeast

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRG645_lexO-AscI_LexA-TF_LEU2MX

Plasmid

#154813

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-E(4130)A2

Plasmid

#168021

Purpose

Expresses E(4130)A2-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

E(4130)A2-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRG714_lexO-AscI_LexA-TF_URA3MX

Plasmid

#154822

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Sc eRF3∆165N (WT)

Plasmid

#175418

Purpose

Encodes His-MBP-TEV-WT eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

∆165N

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
APT3-PTEN (PTEN high)

Plasmid

#168006

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

PTEN-T3'PAP

Use

Synthetic Biology

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1 BSMT1

Plasmid

#106287

Purpose

Expresses BSMT1 in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

BSMT1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrex-b-NLS-hSpCas9

Plasmid

#62543

Purpose

Expression of Cas9 in T. cruzi

Depositor

Rick Tarleton

Article

Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

Insert

NLS-hSpCas9

Use

CRISPR; Trypanosoma cruzi expression

Tags

3xFLAG and NLS

Promoter

Ribo-HX1

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pECFP-Cdc42Q61L

Plasmid

#105294

Purpose

Constitutively active Cdc42

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Cdc42 (CDC42 Human)

Tags

CFP

Expression

Mammalian

Mutation

Q61L

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
APT3-VAMP4

Plasmid

#168004

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP4(I75-L91)-T3'PAP

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pADH143

Plasmid

#90989

Purpose

NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pADH140

Plasmid

#90988

Purpose

NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Inserts

C. maltosa LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPMA3dPlac-PfliC-GFPLVA

Plasmid

#23342

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pVCERC-5 Pvan-dcas9 (Sth3)

Plasmid

#133319

Purpose

expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus thermophilus #3)

Use

Unspecified

Promoter

vanillate

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 12,598 results for: 112