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167,150 results

Showing: 6081 - 6120 of 167150 results

  2. pMasterK

    Plasmid
    #165081
    Purpose
    Episomal expression of reprogramming factors.
    Depositor
    Hideyuki Okano
    Article
    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    lin28 (LIN28A Human)
    EGFP
    HSV TK
    KDM4D (KDM4D Callithrix jacchus)
    GLIS1 (GLIS1 Human)
    Use
    Episomal
    Expression
    Mammalian
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    165081-plasmid-map-sequence-id-444338

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  4. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid
    #208043
    Purpose
    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    SUMO2nc (SUMO2 Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    Q90P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208043-plasmid-map-sequence-id-410267

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  8. TFORF0282

    Plasmid
    #141561
    Purpose
    Lentiviral vector for overexpressing the MEF2C transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MEF2C (MEF2C Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141561-plasmid-map-sequence-id-276819

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  22. pCas12f1

    Plasmid
    #171182
    Purpose
    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1
    Depositor
    Kohsuke Honda
    Article
    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124
    Insert
    Cas12f1
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    cas9 promoter from Streptococcus pyogenes
    Available Since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171182-plasmid-map-sequence-id-336407

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  25. pAC-CANTHipi

    Plasmid
    #53301
    Purpose
    Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtO
    Use
    Low copy number bacterial cloning vector
    Tags
    6His
    Promoter
    Trc
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53301-plasmid-map-sequence-id-99820

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  29. tet-pLKO.puro_shHuR CDS

    Plasmid
    #110411
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110411-plasmid-map-sequence-id-213311

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  32. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

    Plasmid
    #202568
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202568-plasmid-map-sequence-id-429225

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  34. pDEST-N2H-F2-empty vector

    Plasmid
    #125552
    Purpose
    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125552-plasmid-map-sequence-id-252313

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  35. pOpen-DpnIR

    Plasmid
    #165529
    Purpose
    Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DpnI
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165529-plasmid-map-sequence-id-339077

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Showing: 6081 - 6120 of 167150 results