We narrowed to 11,432 results for: 112

1. pACEBac1-Q22YU3-2xStrep

   Plasmid

   #170315

   Purpose

   Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag II

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Q22YU3 (TTHERM_00122270 Tetrahymena thermophila)
   

   Use

   Tags

   Strep-Tag II

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKP1693

   Plasmid

   #167595

   Purpose

   Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.

   Depositor

   Kathleen Postle

   Article

   Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J

   Insert

   FepA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   His6 tag inserted after residue 393 in the mature…

   Promoter

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACEBac1-Q22YU3 del C3-2xStrep

   Plasmid

   #170316

   Purpose

   Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Q22YU3 C-terminal truncation (Delta-C3)
   

   Use

   Tags

   Strep-Tag II

   Expression

   Insect

   Mutation

   Deletion of C-terminal C3-finger

   Promoter

   polyhedrin

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MPC026: pAAV-CAG-FLEX-NovArch-TS-Citrine_TSx3-ER2

   Plasmid

   #153195

   Purpose

   encodes pAAV NovArch

   Depositor

   Adam Cohen

   Article

   Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

   Insert

   NovArch
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-CVm-Sec22b

   Plasmid

   #168029

   Purpose

   Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   eCFP-Sec22b(A131-H190)-mVenus
   

   Use

   Tags

   Hisx6 and myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3_ReBphP-PCM_IRES_sfGFP

   Plasmid

   #166917

   Purpose

   Express ReBphP-PCM and sfGFP

   Depositor

   Andre Stiel

   Article

   Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

   Inserts

   ReBphP-PCM
   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-CVm-VAMP8

   Plasmid

   #168028

   Purpose

   Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   eCFP-VAMP8(G7-R67)-mVenus
   

   Use

   Tags

   Hisx6 and myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCerulean3-31aa-Venus-pEGFP-C3

   Plasmid

   #166754

   Purpose

   For expression of mCerulean3 linked to Venus by 31 amino acid linker

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   mCerulean3-31aa-Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DT048A

   Plasmid

   #159409

   Purpose

   Constitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.J23101-LuxR-pr.LuxR-PFO
   

   Use

   Synthetic Biology

   Tags

   FLAG/FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   J23101 and pLux promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DT046A

    Plasmid

    #159407

    Purpose

    Constitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tet-LuxR-pr.LuxR-PFO
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tet and pLux promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DT047A

    Plasmid

    #159408

    Purpose

    Constitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.T7-LuxR-pr.LuxR-sfGFP-PFO
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tet and pLux promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

    Plasmid

    #164916

    Purpose

    Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    GCN4(UTR)-ZEM(TF)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1 (yeast)

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ad-HisFlag-USP19ER

    Plasmid

    #155244

    Purpose

    for generating adenoviruses expressing the ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Adenoviral

    Tags

    6XHis-3XFlag

    Expression

    Mutation

    H118R- please see dep. comments

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJM554 EF1α 3xFLAG-NLS-VP64-intN in TUPV2

    Plasmid

    #161556

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-intN under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-intN
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJM555 EF1α 3xFLAG-NLS-DsDed-intN in TUPV2

    Plasmid

    #161557

    Purpose

    Constitutive expression of 3xFLAG-NLS-DsDed-intN under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-intN
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJM571 ZF2x6-C 3xFLAG-NLS-VP64-intN in TUPV2

    Plasmid

    #161558

    Purpose

    Inducible expression of 3xFLAG-NLS-VP64-intN under ZF2x6-C the promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-intN
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    ZF2x6-C

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJM575 ZF6x6-C 3xFLAG-NLS-DsDed-ZF10 in TUPV2

    Plasmid

    #161561

    Purpose

    Inducible expression of 3xFLAG-NLS-DsDed-ZF10 under the ZF6x6-C promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-ZF10
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    ZF6x6-C

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJM577 ZF6x6-C 3xFLAG-NLS-DsDed-intN in TUPV2

    Plasmid

    #161563

    Purpose

    Inducible expression of 3xFLAG-NLS-DsDed-intN under the ZF6x6-C promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-intN
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    ZF6x6-C

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJM595 ZF6x6-C intC-ZF1-ZF2-NLS-HA in TUPV2

    Plasmid

    #161569

    Purpose

    Inducible expression of intC-ZF1-ZF2-NLS-HA under the ZF6x6-C promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    intC-ZF1-ZF2-NLS-HA
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    ZF6x6-C

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJM583 EF1α 3xFLAG-NLS-VP64-ZF6 in TUPV2

    Plasmid

    #161544

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF6 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF6
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJB005 CMV 3xFLAG-NLS-intC-ZF2

    Plasmid

    #161550

    Purpose

    Constitutive expression of 3xFLAG-NLS-intC-ZF2 under the CMV promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-intC-ZF2
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJB002 CMV 3xFLAG-NLS-DsDed-ZF1

    Plasmid

    #161532

    Purpose

    Constitutive expression ofCMV 3xFLAG-NLS-DsDed-ZF1 under the CMV promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-ZF1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJM503 EF1α 3xFLAG-NLS-ZF1 in TUPV2

    Plasmid

    #161534

    Purpose

    Constitutive expression of 3xFLAG-NLS-ZF1 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-ZF1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJM506 EF1α 3xFLAG-NLS-DsDed-ZF1 in TUPV2

    Plasmid

    #161536

    Purpose

    Constitutive expression of 3xFLAG-NLS-DsDed-ZF1 gene under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-ZF1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJM507 EF1α 3xFLAG-NLS-DsDed-ZF10 in TUPV2

    Plasmid

    #161537

    Purpose

    Constitutive expression of 3xFLAG-NLS-DsDed-ZF10 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-ZF10
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJM512 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

    Plasmid

    #161538

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJM520 EF1α 3xFLAG-NLS-VP64-ZF10 in TUPV4

    Plasmid

    #161539

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF10 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF10
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJM574 ZF2x6-C 3xFLAG-NLS-DsDed-ZF1 in TUPV2

    Plasmid

    #161541

    Purpose

    Inducible expression of 3xFLAG-NLS-DsDed-ZF1 under the ZF2x6-C promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsDed-ZF1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    ZF2x6-C

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBbdCas9S(-10AC1)_Psyn-sgRNArodA

    Plasmid

    #149659

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC1), Psyn

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBbdCas9S(-10AC2)_Psyn-sgRNArodA

    Plasmid

    #149660

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC2), Psyn

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBbdCas9S(-10TC)_Psyn-sgRNAmreB

    Plasmid

    #149595

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBbdCas9S(-10TC)_Psyn-sgRNAftsI

    Plasmid

    #149591

    Purpose

    all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA-HisFlag-rUSP19ER-CAmut

    Plasmid

    #155237

    Purpose

    for mammalian overexpression of the mutated ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Cys(545) to Ala

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBbdCas9S(-10TC)_Psyn-sgRNArodA

    Plasmid

    #149657

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBbdCas9S(-10TC)_Psyn-sgRNAflaB

    Plasmid

    #149589

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. YCplac33-NAT_NDT1

    Plasmid

    #140593

    Purpose

    Express NDT1 in S. cerevisiae

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    NDT1 promoter and 3'UTR

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSFDuet1-tglA(C51)HIB

    Plasmid

    #134675

    Purpose

    Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglA(C51)
    TglH
    TglI
    TglB
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    insertion of cysteine 51

    Promoter

    T7

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFloat.assemble(AB).LC_v1

    Plasmid

    #133083

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFloat.assemble(AB).HC_v1

    Plasmid

    #133084

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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40. actc1b-mKate2-rab6bb

    Plasmid

    #109654

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab6bb

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab6bb (rab6bb Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. actc1b-mKate2-rab3ab

    Plasmid

    #109636

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab3ab

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab3ab (rab3ab Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. ORF18(R158A_L159A)-3xFLAG pCDNA4

    Plasmid

    #129814

    Purpose

    Expresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Arginine 158 to Alanine and Leucine 159 t…

    Promoter

    CMV

    Available since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. OphA-delta C6-R72K

    Plasmid

    #127683

    Purpose

    Bacterial expression of His-tagged OphA-delta C6-R72K

    Depositor

    James Naismith

    Article

    Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

    Insert

    OphA-delta C6-R72K
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    delta C6-R72K

    Promoter

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. ORF18(G228A)-3xFLAG pCDNA4

    Plasmid

    #129809

    Purpose

    Expresses KSHV ORF18 (G228A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 228 to Alanine

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. ORF18(L29A)-3xFLAG pCDNA4

    Plasmid

    #129777

    Purpose

    Expresses KSHV ORF18 (L29A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Leucine 29 to Alanine

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. ORF18(L37A)-3xFLAG pCDNA4

    Plasmid

    #129779

    Purpose

    Expresses KSVH ORF18 (L37A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Leucine 37 to Alanine

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. ORF18(K75A)-3xFLAG pCDNA4

    Plasmid

    #129784

    Purpose

    Expresses KSHV ORF18 (K75A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 75 to Alanine

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. ORF18(G65A)-3xFLAG pCDNA4

    Plasmid

    #129781

    Purpose

    Expresses KSHV ORF18 (G65A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 65 to Alanine

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. ORF18(L107A)-3xFLAG pCDNA4

    Plasmid

    #129787

    Purpose

    Expresses KSHV ORF18 (L107A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Leucine 107 to Alanine

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. ORF18(W170)-3xFLAG pCDNA4

    Plasmid

    #129803

    Purpose

    Expresses KSHV ORF18 (W170A) with Cterm 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Tryptophan 170 to Alanine

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge